Replication of Oral BK Virus in Human Salivary Gland Cells

Burger-Calderon, Raquel; Madden, Victoria J.; Hallett, Ryan A.; Gingerich, Aaron D.; Nickeleit, Volker; Webster‐Cyriaque, Jennifer

doi:10.1128/jvi.02777-13

Cited by 38 publications

(49 citation statements)

References 64 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although BKPyV is considered a nephrotropic virus, viral proteins and nucleic acids have been detected in several other tissues and organs including bladder, blood, bone, brain and central nervous system (CNS), male and female genitals, heart, lung, liver, skin, salivary glands and spleen (Burger-Calderon et al, 2014;Rekvig & Moens, 2002). Brain and CNS, peripheral blood cells, kidney, lungs, lymphoid organs, tonsils and gastrointestinal tract have been shown to harbour JCPyV (Boothpur & Brennan, 2010;Dörries et al, 2003;Ravichandran & Major, 2006).…”

Section: Introductionmentioning

confidence: 99%

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Moens

Ghelue

Ludvigsen

et al. 2015

Journal of General Virology

View full text Add to dashboard Cite

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines Little is known about cell tropism of the novel HPyVs, and cell cultures allowing virus propagation are lacking. Because viral tropism partially depends on the interaction of cellular transcription factors with the viral promoter, we monitored the promoter activity of all known HPyVs. Therefore, we compared the relative early and late promoter activity of the BK polyomavirus (BKPyV) (WW strain) with the corresponding activities of the other HPyVs in 10 different cell lines derived from brain, colon, kidney, liver, lung, the oral cavity and skin. Our results show that the BKPyV, MCPyV, TSPyV and HPyV12 early promoters displayed the strongest activity in most cell lines tested, while the remaining HPyV had relative low early promoter activity. HPyV12 showed the highest late promoter activity of all HPyVs in most cell lines, but also the BKPyV, MCPyV and TSPyV late promoters belonged to the stronger ones among HPyVs. The HPyVs with weak early promoter activity had in general also weak late promoter activity, except for HPyV10 whose late promoter was relatively strong in six of the 10 cell lines. A 20 bp deletion in the promoter of an HPyV12 variant significantly affected both early and late promoter activity in most cell lines. In conclusion, our findings suggest which cell lines may be suitable for virus propagation and may give an indication of the cell tropism of the HPyVs.

show abstract

Section: Introductionmentioning

confidence: 99%

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Moens

Ghelue

Ludvigsen

et al. 2015

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Some authors suggest that BK virus may be a potential co-factor for HPV in the development of cervical neoplasia (Fraase et al, 2011), especially together with the HPV genotype 16 (Comar et al, 2011). Burger-Calderon et al (2014) suggests a connection between BKPyV and the oral compartment. BKV DNA was detected in tonsilar biopsy specimens and nasopharyngeal aspirates.…”

mentioning

confidence: 99%

Prevalence of Polyoma BK Virus (BKPyV) , Epstein-Barr Virus (EBV) and Human Papilloma Virus (HPV) in Oropharyngeal Cancer

Polz-Gruszka¹,

Morshed²,

Jarzynski³

et al. 2015

Pol J Microbiol

View full text Add to dashboard Cite

A b s t r a c tThe aim of this study was to analyze the prevalence of BK virus, Human Papillomavirus and Epstein-Barr virus in oropharyngeal cancer, and to test our hypothesis that BKV/HPV/EBV co-infection plays a role in oropharyngeal squamous cell carcinoma. The correlation between viral infection, OSCC, anatomic location, pre-treatment staging, evidence of metastases to lymph nodes, and grading was also investigated. The examination samples were collected from 62 patients from paraffin tissue blocks. Males (90.3%) with, smoking (83.9%) and alcohol abuse (67.7%) problems prevailed in the studied group. G2 histological type was recognized in 80.6% cases. T4 (77.4%) and N2 (56.5%) traits occurred in the majority of patients. No cases of metastasis were observed (M0 100%). HPV -24.2%, EBV -27.4% and BKV 17.7% were detected in the studied samples. We observed co-infection EBV/BKV in 8% of cases, HPV/BKV in 4.8%, and HPV/EBV in 9% cases. Only in two cases co-infection of all three viruses was found.

show abstract

“…For example, BK-viremia is not specific to kidney injury and may originate from extra-renal anatomic sites, such as the bladder (during hemorrhagic cystitis) or even salivary glands. 23, 26 Post-kidney transplantation, only a subgroup of patients with BK-viremia will develop definitive, biopsy-proven disease (PVN) while most viremic episodes appear to be self-limiting, without urinary PV-Haufen shedding and without any clinical significance. In cases of PVN the level of BK-viremia by PCR only imperfectly reflects the severity of intra-renal disease.…”

Section: Discussionmentioning

confidence: 99%

The Noninvasive Urinary Polyomavirus Haufen Test Predicts BK Virus Nephropathy in Children After Hematopoietic Cell Transplantation

et al. 2016

Self Cite

View full text Add to dashboard Cite

Background After hematopoietic cell transplantation (HCT), polyoma-BK virus is associated with hemorrhagic cystitis and also with polyomavirus nephropathy (PVN). However, the true burden of post-HCT PVN is unknown because kidney biopsies are avoided due to their bleeding risk. The novel, non-invasive urinary PV-Haufen test detects PVN in kidney transplant recipients with >95% positive/negative predictive values. We hypothesized that the detection of PV-Haufen in voided urine samples–a positive PV-Haufen test–was also clinically significant after HCT. Methods We examined 21 suitable urine samples from 14 patients (median age 15 years, 71.4% male) who were selected from repositories for having varying degrees of BK-viremia (range 0-1.0×108 copies/mL), hemorrhagic cystitis (present/absent) and data on kidney function. Urine samples were obtained a median of 88 days post-HCT. Results PV-Haufen were detected in 5/14 patient (35.7%) and 7/21 (33.3%) urine samples, with histologic confirmation of PVN in one autopsy specimen. After a median of 285 days post-HCT, patients with PV-Haufen had an increased risk of dialysis-dependent renal failure (p<0.05). All three dialysis-dependent patients had PV-Haufen and died. The presence of urinary PV-Haufen was not significantly correlated with hemorrhagic cystitis. From the 16 urines collected during BK-viremia, 43.8% were PV-Haufen positive and 56.2% negative. PV-Haufen were not present in the five urines from patients without concomitant BK-viremia. Conclusions In this proof-of-concept study, a positive PV-Haufen test was only seen in some patients with BK-viremia, and was not associated with hemorrhagic cystitis. The detection of PV-Haufen suggests underlying PVN with an increased risk of kidney failure and dialysis.

show abstract

Replication of Oral BK Virus in Human Salivary Gland Cells

Cited by 38 publications

References 64 publications

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Early and late promoters of BK polyomavirus, Merkel cell polyomavirus, Trichodysplasia spinulosa-associated polyomavirus and human polyomavirus 12 are among the strongest of all known human polyomaviruses in 10 different cell lines

Prevalence of Polyoma BK Virus (BKPyV) , Epstein-Barr Virus (EBV) and Human Papilloma Virus (HPV) in Oropharyngeal Cancer

The Noninvasive Urinary Polyomavirus Haufen Test Predicts BK Virus Nephropathy in Children After Hematopoietic Cell Transplantation

Contact Info

Product

Resources

About