Replication of poliovirus and measles virus in cultures of human lymphoblastoid and of burkitt lymphoma cell lines

Fagræus, Astrid; Böttiger, M; Heller, L.; Norrby, Erling

doi:10.1007/bf01317338

Cited by 13 publications

(8 citation statements)

References 18 publications

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“…These observations confirm what was previously shown for nonrecombinant MV-infected Vero cells (2) and contrast with the results of two studies (16,17) which observed severe actin disruption during MV infection of a human lung cell line. We have confirmed that MVeGFP infection of Vero cells causes no disruption of the actin cytoskeleton (data not shown).…”

Section: Measles Virus (Mv) Is a Morbillivirus Which Belongs To Thecontrasting

confidence: 57%

“…One group has reported a striking decrease in the overall number of actin bundles in human fibroblasts infected with MV. They also show a similar disruption upon infection with other Paramyxoviridae (16,17). Contrary to this, a second group has not been able to demonstrate alterations to the actin cytoskeleton in MV-infected Vero cells (2).…”

Section: Measles Virus (Mv) Is a Morbillivirus Which Belongs To Thementioning

confidence: 79%

“…The diffuse nature of EGFP autofluorescence makes MVeGFP an ideal candidate for assessing the effects of virus replication on the cytoskeletons of MVeGFP-infected cells. As tubulin has been shown to stimulate MV RNA synthesis in vitro (36) and the fate of actin within MV-infected cells remains unclear (2,16,17), we decided to use MVeGFP to investigate the effects of MV infection on the cytoskeleton. Confocal scanning laser microscopy (CSLM) was used to gain maximal resolution in dually labeled specimens.…”

Section: Measles Virus (Mv) Is a Morbillivirus Which Belongs To Thementioning

confidence: 99%

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Measles Virus-Induced Disruption of the Glial-Fibrillary-Acidic Protein Cytoskeleton in an Astrocytoma Cell Line (U-251)

Duprex

McQuaid

Rima

2000

J Virol

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A recombinant measles virus which expresses enhanced green fluorescent protein (MVeGFP) has been used to infect two astrocytoma cell lines (GCCM and U-251) to study the effect of virus infection on the cytoskeleton. Indirect immunocytochemistry was used to demonstrate the cellular localization of the cytoskeletal components. Enhanced green fluorescent protein autofluorescence was used to identify measles virus-infected cells. No alteration of the actin, tubulin, or vimentin components of the cytoskeleton was observed in either cell type, whereas a disruption of the glial-fibrillary-acidic protein filament (GFAP) network was noted in MVeGFPinfected U-251 cells. The relative amounts of GFAP present in infected and uninfected U-251 cells were quantified by image analysis of data sets obtained by confocal microscopy by using vimentin, another intermediate filament on which MVeGFP has no effect, as a control.

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Section: Measles Virus (Mv) Is a Morbillivirus Which Belongs To Thecontrasting

confidence: 57%

Section: Measles Virus (Mv) Is a Morbillivirus Which Belongs To Thementioning

confidence: 79%

Section: Measles Virus (Mv) Is a Morbillivirus Which Belongs To Thementioning

confidence: 99%

See 1 more Smart Citation

Measles Virus-Induced Disruption of the Glial-Fibrillary-Acidic Protein Cytoskeleton in an Astrocytoma Cell Line (U-251)

Duprex

McQuaid

Rima

2000

J Virol

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“…Several cell lines derived from hematopoetic tissues, BL lines included, have been reported to be resistant to poliovirus infection (30,31). Northern blot analyses for hPVR mRNA in RNAs of 12 BL cell lines were negative (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…It is known that certain cell lines of the hematopoetic lineage cannot be infected with poliovirus (30,31). Most likely, these cells do not express hPVR polypeptides.…”

Section: Cloning Of the 5ј-region Of The Hpvrmentioning

confidence: 99%

The Promoters for Human and Monkey Poliovirus Receptors

Solecki

Schwarz

Wimmer

et al. 1997

Journal of Biological Chemistry

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The cellular receptors for poliovirus (PVR) are glycoproteins belonging to the immunoglobulin superfamily. Functional receptors for poliovirus are only expressed by primates; known rodent homologues lack the ability to bind virus due to amino acid differences. Human poliovirus infections are targeted to the gastrointestinal tract and, rarely, to motor neurons in the central nervous system. Available evidence suggests that poliovirus uses only one cellular receptor, implying that the tissue tropism of poliovirus is likely to be related to the expression of the human PVR (hPVR). However, low levels of expression of hPVR-specific mRNAs can be detected in many human tissues other than the apparent target cells. The nonpathogenic function of hPVR is unknown. For a study of the transcriptional control of hPVR expression, we have isolated and characterized the promoter of the hPVR gene. Deletion analysis defined an approximately 280 base pair minimal promoter fragment that: 1) lacks TATA-and CAAT-like elements, 2) is distinguished by a high GC content, and 3) promotes transcription at multiple start sites. The pattern of activity caused by transfection of serial 5 -and 3 -promoter deletions is almost identical in HEp2, HeLa, COS-1, and mouse L929 cells, indicating a similar transcriptional regulation of the hPVR promoter in these cell lines. However, on transfection of Raji cells, a Burkitt's lymphoma cell line harboring a transcriptionally inactive hPVR gene, all promoter reporter constructs tested exerted only residual activity. These results suggest that the cis-element(s) governing cell type-specific hPVR expression resides in the minimal promoter region. We also report the sequences of the promoters of two monkey homologues to hPVR (AGM␣1 and AGM␣2).

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Cellular tropism of human enterovirus D species serotypes EV‐94, EV‐70, and EV‐68 in vitro: Implications for pathogenesis

Smura¹,

Ylipaasto²,

Klemola³

et al. 2010

Journal of Medical Virology

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Enterovirus 94 (EV‐94) is an enterovirus serotype described recently which, together with EV‐68 and EV‐70, forms human enterovirus D species. This study investigates the seroprevalences of these three serotypes and their abilities to infect, replicate, and damage cell types considered to be essential for enterovirus‐induced diseases. The cell types studied included human leukocyte cell lines, primary endothelial cells, and pancreatic islets. High prevalence of neutralizing antibodies against EV‐68 and EV‐94 was found in the Finnish population. The virus strains studied had wide leukocyte tropism. EV‐94 and EV‐68 were able to produce infectious progeny in leukocyte cell lines with monocytic, granulocytic, T‐cell, or B‐cell characteristics. EV‐94 and EV‐70 were capable of infecting primary human umbilical vein endothelial cells, whereas EV‐68 had only marginal progeny production and did not induce cytopathic effects in these cells. Intriguingly, EV‐94 was able to damage pancreatic islet β‐cells, to infect, replicate, and cause necrosis in human pancreatic islets, and to induce proinflammatory and chemoattractive cytokine expression in endothelial cells. These results suggest that HEV‐D viruses may be more prevalent than has been thought previously, and they provide in vitro evidence that EV‐94 may be a potent pathogen and should be considered a potentially diabetogenic enterovirus type. J. Med. Virol. 82:1940–1949, 2010. © 2010 Wiley‐Liss, Inc.

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Replication of poliovirus and measles virus in cultures of human lymphoblastoid and of burkitt lymphoma cell lines

Cited by 13 publications

References 18 publications

Measles Virus-Induced Disruption of the Glial-Fibrillary-Acidic Protein Cytoskeleton in an Astrocytoma Cell Line (U-251)

Measles Virus-Induced Disruption of the Glial-Fibrillary-Acidic Protein Cytoskeleton in an Astrocytoma Cell Line (U-251)

The Promoters for Human and Monkey Poliovirus Receptors

Cellular tropism of human enterovirus D species serotypes EV‐94, EV‐70, and EV‐68 in vitro: Implications for pathogenesis

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