Replication of rat coronavirus in a rat cell line, LBC

Hirano, Norio; Ono, Koji; Sada, Y.; Inoue, Akira; Murakami, Tasuku; Takamaru, H

doi:10.1007/bf01314238

Cited by 17 publications

(12 citation statements)

References 4 publications

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“…Reproducibility and accuracy of the plaque test were good. The infectivity titers were in the same range as described for other coronaviruses (Robb and Bond, 1979b;Schmidt et al, 1979;Vautherot, 1981;Hirano et al, 1985).…”

Section: Discussionmentioning

confidence: 94%

Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)

Hofmann

Wyler

1989

Veterinary Microbiology

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The porcine epidemic coronavirus (PEDV), tentatively classified as a coronavirus, was adapted to Vero cells and a plaque test developed for infectivity titration, allowing us to test the biological and biophysical properties of the virus. Growth kinetics showed peak titers of 10(5.5) plaque-forming units ml-1 15 h after infection. Filtration experiments and electron microscopy revealed a particle diameter between 100 and 200 nm. The buoyant density of the virus was 1.18. The particle lost its infectivity on treatment with lipid solvents. Virus replication could not be inhibited by 5-iodo-2'-deoxyuridine. PEDV was moderately stable at 50 degrees C, but heat sensitivity was not altered by divalent cations. At 4 degrees C, the virus was stable between pH 5.0 and 9.0, but at 37 degrees C stability was restricted to the pH range 6.5-7.5. Viral infectivity was not impaired by ultrasonication or by multiple freezing and thawing. PEDV was not neutralized by transmissible gastroenteritis virus antiserum. On the basis of the tests carried out, PEDV is a pleomorphic, enveloped RNA virus with a particle diameter of approximately 150 nm and a buoyant density of 1.18. Infectivity depends on the presence of trypsin, and infected cells show a tendency to fuse and to form syncytia. All of these properties, as well as its physicochemical characteristics, allow PEDV to be classified as a coronavirus.

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Section: Discussionmentioning

confidence: 94%

Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)

Hofmann

Wyler

1989

Veterinary Microbiology

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“…We have had very poor success producing the primary rat kidney cells, mentioned by Parker et al (1970), that are only susceptible to virus infections when used within one week after initial preparation of the cells from rat kidneys. The LBC cell line that supports the replication of rat coronavirus could not be obtained from those who developed it (Hirano et al, 1985). It is assumed that the in vivo antiviral activity against rat coronavirus is correlated with decreases in virus titres, as was shown for EMC virus.…”

Section: Discussionmentioning

confidence: 99%

Intranasal Treatment of Picornavirus and Coronavirus Respiratory Infections in Rodents Using 7-Thia-8-Oxoguanosine

Smee¹,

Alaghamandan²,

Bartlett³

et al. 1990

Antivir Chem Chemother

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SummarySince common cold viruses are responsive to interferon, we developed two animal models to test the efficacy of the interferon inducer 7-thia-8-oxoguano'" sine: (i) an intranasal coronavirus infection in suckling rats; and (ii) an intranasal encephalomyocarditis (EMC) virus infection in adult mice. Concentrations of 0.3 and 1% 7-thia-8-oxoguanosine delivered intranasally to rats 24 and 18 hours before virus inoculation were highly protective against the otherwise lethal coronavirus infection. A 1% concentration of drug administered 4 and 8 hours after virus challenge increased mean survival times of rats but did not increase numbers of survivors. Intranasal treatment of an EMC infection produced moderate improvements in mean survival times and survival. Titrations of EMC virus indicated >300-fold reductions in nasal titres in drug-treated relative to placebo control animals on days 2-4 following virus challenge. The distribution of r 4 C]-7-thia-8-oxoguanosine was determined shortly after intranasal delivery to mice and rats. Approximately 50% of total doses were deposited in the inner noses and mouths of both species. Most of the rest was found on/in the outer noses, stomachs, tracheas, oesophagi, and lungs. By analogy, the infecting viruses were deposited on/in the same organs and tissues of each species. The results suggest that containment of the viruses primarily occurred in the nasopharyngeal area prior to their spread to the lungs (rat coronavirus) or the brain (EMC), where fatal pathologies were manifest. Intranasal application of an interferon-inducing nucleoside analogue represents a new approach for the study of treatment of the common cold.

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“…The LBC cells, established from a spontaneous mammary tumor developed in a Lewis rat (3), were grown at 37 ° C in Eagle's minimum essential medium (MEM) containing 10 per cent fetal calf serum (FCS) and kanamycin (0.06 mg/ml). The FCS concentration was reduced to 5 per cent for maintaining the cells and harvesting the virus.…”

mentioning

confidence: 99%

Replication of sialodacryoadenitis virus of rat in LBC cell culture

Hirano

Takamaru

Ono

et al. 1986

Archives of Virology

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Replication of rat coronavirus in a rat cell line, LBC

Abstract: Rat coronavirus readily propagated and induced marked cytopathic effect in a rat cell line, LBC cell culture, which provided a sensitive, practical assay system for viral infectivity and neutralizing antibody, and a satisfactory source of the virus.

Cited by 17 publications

References 4 publications

Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)

Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)

Intranasal Treatment of Picornavirus and Coronavirus Respiratory Infections in Rodents Using 7-Thia-8-Oxoguanosine

Replication of sialodacryoadenitis virus of rat in LBC cell culture

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