Replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in HuH7 hepatocarcinoma cell line

Abstract

Detection of viral antigens and isolation methods has long been used for the diagnosis of respiratory virus infections. The objective was to determine the ability of HuH7 cells to support the replication of prototype and wild strains of respiratory viruses. The cell culture-adapted strains of influenza viruses A and B, parainfluenza viruses 1-4, respiratory syncytial viruses A and B, both strains of the human metapneumoviruses, numerous rhinoviruses, most of the adenoviruses, coronaviruses 229E and OC43, and a number of enteroviruses (poliovirus type 3, coxsackie virus B1, echovirus type 30) replicate in HuH7. The kinetic study of the replication of influenza A and B viruses showed that there were infected cells in HuH7 and MDCK lines as early as 24 hr post-infection. However, the replication of influenza A and B viruses was more rapid and intense on MDCK cells than on HuH7 cells. During the three winters of 1999-2000, 2000-2001, and 2001-2002, of the 1,226 (23.3%) direct fluorescent assay-positive nasal aspirates from children admitted to hospital, 788 were positive for respiratory syncytial virus, 228 for influenza virus, 133 for parainfluenza virus, and 77 for adenovirus. Of the 4,032 direct fluorescent assay-negative nasal aspirates, 571 virus isolates were identified by using HuH7 cell culture; 272 rhinoviruses, 100 influenza viruses A and B, 85 enteroviruses, 40 adenoviruses, 35 coronaviruses, 31 parainfluenza viruses, and 10 respiratory syncytial viruses. Interestingly, 100/328 (30.5%) influenza viruses A and B, 40/189 (21.1%) adenoviruses, and 31/164 (19%) parainfluenza viruses type 1-3, not detected by direct fluorescent assay, were identified by isolation in HuH7 cell culture.

Human Cytokinome Analysis for Interferon Response

“…It is a wellestablished human hepatocyte-derived liver cell line model that responds to all types of IFNs and to many cytokines and chemokines (21)(22)(23)(24)(25) and is susceptible to several viruses, including liver and respiratory viruses (7,31). It is important to note that despite the choice of Huh-7 liver cells based on their broad response to a wide variety of IFNs and cytokines, our study is limited by the repertoire of responsive receptors in the liver cells.…”

Norwalk Virus RNA Is Infectious in Mammalian Cells

“…However, our results are consistent with data published by Chang et al (7), which show that Huh-7 cells can harbor a NV RNA replicon, suggesting that these cells are highly susceptible to NV replication. Not only have Huh-7 cells been very successful in allowing replication of other fastidious gastrointestinal viruses such as HCV (29,42,45,46) and hepatitis A virus (28), but they have also been shown to support growth of many respiratory RNA viruses (11).…”

Diagnostic moléculaire des infections respiratoires à entérovirus : apport de la PCR et du génotypage pour une meilleure approche de la circulation des souches en Basse-Normandie au cours de l’année 2008

“…respiratoires dont les EV et RV, aucun ECP caracté ristique de virus n'est observable sur cette ligné e cellulaire [19].…”