Replication of two coleopteran nodaviruses in the coleopteran cell line DSIR-HA-1179 fromHeteronychus aratorand the dipteran cell line fromDrosophila melanogasterline 1

Abstract

Two nodaviruses, both isolated from New Zealand scarab beetles, replicated in DSIR-HA-I 179 (HA) cells derived from the black beetle, Heteronychus arator (Coleoptera: Scarabaeidae). Black beetle virus and Flock House virus, isolated from the grass grub (Costelytra zealandica) produced a characteristic cytopathic effect in cells.

Iridovirus infection of cell cultures from the Diaprepes root weevil, Diaprepes abbreviatus

“…A major use of insect cell cultures is for the examination of virus/cell interactions in an environment wherein insect colonies are not necessary (Crawford, et al ., 1984, Funk et al ., 2001, Gopinadhan et al ., 1990, Hunter and Polston 2001, Kimura 1984). There are other potential benefits to the development of new insect cell cultures.…”

“…Iridoviruses have been reported to infect the Boll weevil, Anthonomus grandis, Boheman (McLaughlin et al ., 1972) and have been identified from other scarab beetles such as the Black beetle, Heteronychus arator Say (Carey et al ., 1978, Longworth et al ., 1979) and Sericesthis pruinosa (Dalman) (Day and Mercer 1964). Nodaviruses have also been isolated from scarab beetles in New Zealand (Crawford et al ., 1984) and a nuclear polyhedrosis virus has reportedly been isolated from the Boll weevil (Ryel and Cline 1970). As more insect viruses are discovered the possibility of developing an efficient, viral based, management system against the Diaprepes root weevil, comes closer to being realized.…”

Iridovirus infection of cell cultures from the Diaprepes root weevil, Diaprepes abbreviatus

“…Iridoviruses have been reported to infect the Boll weevil, Anthonomus grandis, Boheman (McLaughlin et al, 1972) and have been identified from other scarab beetles such as the Black beetle, Heteronychus arator Say (Carey et al, 1978, Longworth et al, 1979 and Sericesthis pruinosa (Dalman) (Day and Mercer 1964). Nodaviruses have also been isolated from scarab beetles in New Zealand (Crawford et al, 1984) and a nuclear polyhedrosis virus has reportedly been isolated from the Boll weevil (Ryel and Cline 1970). As more insect viruses are discovered the possibility of developing an efficient, viral based, management system against the Diaprepes root weevil, comes closer to being realized.…”