Replication profile of <i>Saccharomyces cerevisiae</i> chromosome VI

Friedman, Katherine L.; Brewer, Bonita J.; Fangman, Walton L.

doi:10.1046/j.1365-2443.1997.1520350.x

Cited by 153 publications

(178 citation statements)

References 38 publications

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“…Figure 1A and Supplemental Figure S4). At 2 h of meiosis, both Spo11-FLAG and Mre11-FLAG began to be localized in concert to an early replicating region of chromosome VI (Friedman et al, 1997;Yamashita et al, 1997;Raghuraman et al, 2001;Mori and Shirahige, 2007), including the very early mitotic and meiotic replication origins autonomously replicating sequences (ARS)606 and ARS607 (Figure 1, B and C). As premeiotic DNA replication further proceeded, they gradually localized to the chromosomal arm regions harboring comparatively late-replicating origins (Pearson r of signal log 2 ratio between Spo11-FLAG and Mre11-FLAG is 0.85 at 2 h and 0.72 at 3 h; Figure 1, B and C).…”

Section: Localization Of Spo11 To Chromosomal Arms During Premeiotic mentioning

confidence: 99%

Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosomes

Kugou

Fukuda

Yamada

et al. 2009

MBoC

109

143

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Spo11-mediated DNA double-strand breaks (DSBs) that initiate meiotic recombination are temporally and spatially controlled. The meiotic cohesin Rec8 has been implicated in regulating DSB formation, but little is known about the features of their interplay. To elucidate this point, we investigated the genome-wide localization of Spo11 in budding yeast during early meiosis by chromatin immunoprecipitation using high-density tiling arrays. We found that Spo11 is dynamically localized to meiotic chromosomes. Spo11 initially accumulated around centromeres and thereafter localized to arm regions as premeiotic S phase proceeded. During this stage, a substantial proportion of Spo11 bound to Rec8 binding sites. Eventually, some of Spo11 further bound to both DSB and Rec8 sites. We also showed that such a change in a distribution of Spo11 is affected by hydroxyurea treatment. Interestingly, deletion of REC8 influences the localization of Spo11 to centromeres and in some of the intervals of the chromosomal arms. Thus, we observed a lack of DSB formation in a region-specific manner. These observations suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation.

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Section: Localization Of Spo11 To Chromosomal Arms During Premeiotic mentioning

confidence: 99%

Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosomes

Kugou

Fukuda

Yamada

et al. 2009

MBoC

109

143

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“…Importantly, the ability of 2D gel assays to discriminate between active (bubble arc) and passive replication (Y arc) revealed that most of the yeast origins are intrinsically inefficient, as they fire in only a fraction of the cell cycles. Later on, extensive analyses of chromosomes III and VI by 2D gel electrophoresis confirmed that the budding yeast genome contains an excess of replication origins (Friedman et al 1997;Yamashita et al 1997). As for other eukaryotes, S. cerevisiae origins vary both in their timing and efficiency of initiation.…”

Section: Dna Replication Programs: the Yeast Paradigmmentioning

confidence: 99%

Defining replication origin efficiency using DNA fiber assays

2009

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The timely duplication of eukaryotic genomes depends on the coordinated activation of thousands of replication origins distributed along the chromosomes. Origin activation follows a temporal program that is imposed by the chromosomal context and is under the control of S-phase checkpoints. Although the general mechanisms regulating DNA replication are now well-understood at the level of individual origins, little is known on the coordination of thousands of initiation events at a genome-wide level. Recent studies using DNA combing and other single-molecule assays have shown that eukaryotic genomes contain a large excess of replication origins. Most of these origins remain "dormant" in normal growth conditions but are activated when fork progression is impeded. In this review, we discuss how DNA fiber technologies have changed our view of eukaryotic replication programs and how origin redundancy contributes to the maintenance of genome integrity in eukaryotic cells.

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“…Each origin of DNA replication is strictly controlled to fire only once in the cell cycle in the fixed sequential order (1,2). This temporal program for origin firing is utilized for the maintenance of genome integrity through DNA replication checkpoint mechanism (3,4).…”

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confidence: 99%

“…It contains ten ARSs, ARS601-609, of which nine ARSs are active as replication origins that comprise five active replicons in mitotic S-phase (1,2,15). Thus, three origins (ARS605, 606, and 607) are used in high frequency, four (ARS601/2, 603, 603.5, and 609) are in intermediate frequency, and two (ARS604 and 608) are in low frequency, less than 5% of cell cycles.…”

mentioning

confidence: 99%

“…Thus, three origins (ARS605, 606, and 607) are used in high frequency, four (ARS601/2, 603, 603.5, and 609) are in intermediate frequency, and two (ARS604 and 608) are in low frequency, less than 5% of cell cycles. Replication of each arm of chromosome starts from one major origin (ARS605 and 607 on the left and the right arm, respectively) and extends sequentially in both directions (1,2,6). During mitotic growth, all of these replication origins are bound by ORC (16,17).…”

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confidence: 99%

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Perturbation of the Activity of Replication Origin by Meiosis-specific Transcription

Mori

Shirahige

2007

Journal of Biological Chemistry

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We have determined the activity of all ARSs on the Saccharomyces cerevisiae chromosome VI as chromosomal replication origins in premeiotic S-phase by neutral/neutral two-dimensional gel electrophoresis. The comparison of origin activity of each origin in mitotic and premeiotic S-phase showed that one of the most efficient origins in mitotic S-phase, ARS605, was completely inhibited in premeiotic S-phase. ARS605 is located within the open reading frame of MSH4 gene that is transcribed specifically during an early stage of meiosis. Systematic analysis of relationships between MSH4 transcription and ARS605 origin activity revealed that transcription of MSH4 inhibited the ARS605 origin activity by removing origin recognition complex from ARS605. Deletion of UME6, a transcription factor responsible for repressing MSH4 during mitotic S-phase, resulted in inactivation of ARS605 in mitosis. Our finding is the first demonstration that the transcriptional regulation on the replication origin activity is related to changes in cell physiology. These results may provide insights into changes in replication origin activity in embryonic cell cycle during early developmental stages.Eukaryotic chromosomes consist of multiple replication units. Each origin of DNA replication is strictly controlled to fire only once in the cell cycle in the fixed sequential order (1, 2). This temporal program for origin firing is utilized for the maintenance of genome integrity through DNA replication checkpoint mechanism (3, 4). It was shown that a checkpoint effector kinase Rad53 repressed firing of late origins when replication was perturbed by hydroxy urea or methyl methane sulfonate. Furthermore, recent genome-wide studies of eukaryotic chromosomal DNA replication using Saccharomyces cerevisiae have provided us with a kinetic map of progression of DNA replication along the chromosome (5, 6). However, these studies were restricted to DNA replication during mitotic cell cycle and little is known about how these multiple replication origins behave under different cell cycle, i.e. meiotic cell cycle.The decision to enter the meiotic cell cycle is made in G 1 phase and this affects the way in which the G 1 /S transition is controlled. In budding yeast (S. cerevisiae), poor nutrient conditions are the cue to embark on the meiotic cell cycle, which culminates in the production of spores (7). The replication of chromosome is the first detectable cytological event in meiosis. The coordinated synthesis of genomic DNA requires multiple levels of regulation and a large number of gene products. Genetic analysis in S. cerevisiae indicates that the replicative machinery used to synthesize DNA in vegetative cells is also required for the duplication of chromosome in meiosis (8 -10).In S. cerevisiae, mitotic S-phase and premeiotic S-phase appear to be differently regulated. For example, premeiotic S-phase is 1.5-2 times longer than mitotic S-phase (11). However, replication kinetics as measured by neutral/neutral twodimensional gel electrophoresis of 100-kb s...

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Replication profile of Saccharomyces cerevisiae chromosome VI

Cited by 153 publications

References 38 publications

Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosomes

Rec8 Guides Canonical Spo11 Distribution along Yeast Meiotic Chromosomes

Defining replication origin efficiency using DNA fiber assays

Perturbation of the Activity of Replication Origin by Meiosis-specific Transcription

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