2015
DOI: 10.1186/s13148-015-0057-5
|View full text |Cite
|
Sign up to set email alerts
|

Replicative senescence is associated with nuclear reorganization and with DNA methylation at specific transcription factor binding sites

Abstract: BackgroundPrimary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation (DNAm) at specific sites in the genome. The mechanism that drives senescence-associated DNAm changes remains unknown - it may involve stochastic DNAm drift due to imperfect maintenance of epigenetic marks or it is… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

4
53
0

Year Published

2015
2015
2023
2023

Publication Types

Select...
6
3

Relationship

0
9

Authors

Journals

citations
Cited by 57 publications
(57 citation statements)
references
References 71 publications
4
53
0
Order By: Relevance
“…Thus, the only significant effect on telomere length which could be ascribed to STZ in ADIPO-P2 cells was observed 10 d after treatment. Furthermore, the dramatic changes detected in telomere length in control cells could be ascribed to the loss of proliferative potential during in vitro cell culture, after a limited number of cell divisions [33]. The reduction in telomere length during the first passages could have a definitive role in the loss of chromosomal integrity among the in vitro cell culture expansion.…”
Section: Telomere Length In Stz-treated and Untreated Cellsmentioning
confidence: 98%
See 1 more Smart Citation
“…Thus, the only significant effect on telomere length which could be ascribed to STZ in ADIPO-P2 cells was observed 10 d after treatment. Furthermore, the dramatic changes detected in telomere length in control cells could be ascribed to the loss of proliferative potential during in vitro cell culture, after a limited number of cell divisions [33]. The reduction in telomere length during the first passages could have a definitive role in the loss of chromosomal integrity among the in vitro cell culture expansion.…”
Section: Telomere Length In Stz-treated and Untreated Cellsmentioning
confidence: 98%
“…The reduction in telomere length during the first passages could have a definitive role in the loss of chromosomal integrity among the in vitro cell culture expansion. Thus, cells cultured in vitro in the long term for several passages may present changes in the telomere length, a phenomenon usually observed in in vitro cultured cells [33]. Therefore, the comparison between control and exposed cells was analyzed independently at each time point investigated.…”
Section: Telomere Length In Stz-treated and Untreated Cellsmentioning
confidence: 99%
“…Both genetic and environmental factors have been proposed to contribute to age-associated changes in DNA methylation (Fraga et al, 2005;Gronniger et al, 2010;Hannum et al, 2013;Horvath, 2013). As we mentioned above, the mechanisms underlying these effects may be distinct for sites increasing vs. ones decreasing in methylation with aging, and they may include the upregulation and binding of senescence-associated transcription factors (Hanzelmann et al, 2015) and the downregulation of DNMT1 (Li et al, 2010). Furthermore, it is currently unclear whether age-associated changes in the methylome are merely an epiphenomenon or play a causal role in the development of aging-related disease.…”
Section: Dna Methylation and Aging-related Phenotypesmentioning
confidence: 92%
“…Notably, the HDF model excels in many of the important experimental considerations listed above: (1) they are relatively easy to obtain from ambulatory skin biopsies (Vangipuram et al, 2013, Wang et al, 2004), (2) their maintenance does not require significant equipment, chemical or human resources; (3) they can be easily propagated for long periods of time without genetic manipulation or external growth factors and stored in liquid nitrogen for years (Auburger et al, 2012;); and (4) HDF cultures consist of mitotic and post-mitotic, uniform fibroblast cells (Bayreuther et al, 1991) and retain genetic stability for 15–20 passages (Hänzelmann et al, 2015), making a single biopsy sufficient for hundreds of experiments. All these considerations make HDF cultures an appealing, cost-effective experimental model.…”
Section: Patient Biomaterials Models Advantages and Limitationsmentioning
confidence: 99%