Reply to W. Mohr's comment on the paper Intercellular network in articular cartilage of pigs with experimentally induced arthritis

R, Denecke

doi:10.1007/bf00541374

Rheumatol Int

1986

DOI: 10.1007/bf00541374

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Reply to W. Mohr's comment on the paper Intercellular network in articular cartilage of pigs with experimentally induced arthritis

Denecke R¹

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1995

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The biological reality of the interlacunar network in the embryonic, cartilaginous, skeleton: A thiazine dye/absolute ethanol/LR White resin protocol for visualizing the network with minimal tissue shrinkage

Lawton

Oswald

McClure

1995

Journal of Microscopy

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Summary Third toe phalanges of chicks aged 8–13 days in ovo and 7‐day post‐natal rat femoral growth plate were examined to determine whether the interlacunar network (IN), a structure with no lipoprotein membrane component or cytoplasmic organelles, is a genuine component of young growth cartilage. In chick phalanges dehydrated by 70% (v/v) ethanol and LR White resin, variable metachromatic staining of the interlacunar network by toluidine blue and red staining by picro‐Sirius red indicate the presence of glycosaminoglycans and collagen. The network in phalanges dehydrated by 80% (v/v) ethanol appears little different; however, the network is much less widely detectable in phalanges dehydrated by 90% (v/v) ethanol and, after dehydration by absolute ethanol, is almost completely undetectable. In contrast, when the young cartilage is permeated by a thiazine dye such as toluidine blue, using a solution of dye in the aldehyde fixative, the network is widely detectable, following dehydration by absolute ethanol, both in chick phalanges and in rat growth plate. Comparison of projected areas shows that the extent to which whole chick feet are found to have shrunk, by the time that they are photographed under LR White resin, is determined principally by the extent of dehydration, by 70% (v/v) or absolute ethanol; post‐shrinkage areas are 33% or 35% of areas measured in buffer for 70% (v/v) ethanol/LR White resin and 71% or 75% for absolute ethanol/LR White resin (the higher value in each is for the toluidine blue treatment). The network is thus present in radically shrunk tissue, but, significantly, is also fully represented in tissue shrunk by only a conventional margin and is therefore not produced as an artefact by exceptional tissue shrinkage as has been suggested.

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The biological reality of the interlacunar network in the embryonic, cartilaginous, skeleton: A thiazine dye/absolute ethanol/LR White resin protocol for visualizing the network with minimal tissue shrinkage

Lawton

Oswald

McClure

1995

Journal of Microscopy

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Reply to W. Mohr's comment on the paper Intercellular network in articular cartilage of pigs with experimentally induced arthritis

Cited by 1 publication

References 1 publication

The biological reality of the interlacunar network in the embryonic, cartilaginous, skeleton: A thiazine dye/absolute ethanol/LR White resin protocol for visualizing the network with minimal tissue shrinkage

The biological reality of the interlacunar network in the embryonic, cartilaginous, skeleton: A thiazine dye/absolute ethanol/LR White resin protocol for visualizing the network with minimal tissue shrinkage

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