Reply to Zhang &amp; Gilbert (2017): Comment on ‘Measurement and relevance of maximum metabolic rate in fishes by Norin &amp; Clark (2016)’

Norin, Tommy

doi:10.1111/jfb.13353

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…repeatable; Pearson's r and Spearman's ρ ≥0.77; P <0.05; Zhang et al, 2020 ), whereas neither was significantly correlated with Ṁ O 2 ,recovery . In contrast, a study on juvenile barramundi ( Lates calcarifer ) showed that Ṁ O 2 ,chase was not as high as Ṁ O 2 ,recovery measured immediately post-chase ( Norin and Clark, 2016 , 2017 ). Clearly, more validation is required, but using a modified ‘chase respirometer’ might offer researchers a tool that is higher throughput, lower cost and more portable than traditional swim tunnel respirometry, while also providing estimates of peak Ṁ O 2 that may be similar to Ṁ O 2 ,swim in mean and repeatability.…”

Section: Improving Methods To Measure Peak ṁ Omentioning

confidence: 82%

Estimating maximum oxygen uptake of fishes during swimming and following exhaustive chase – different results, biological bases and applications

Rees,

Reemeyer,

Binning

et al. 2024

Journal of Experimental Biology

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The maximum rate at which animals take up oxygen from their environment (ṀO2,max) is a crucial aspect of their physiology and ecology. In fishes, ṀO2,max is commonly quantified by measuring oxygen uptake either during incremental swimming tests or during recovery from an exhaustive chase. In this Commentary, we compile recent studies that apply both techniques to the same fish and show that the two methods typically yield different mean estimates of ṀO2,max for a group of individuals. Furthermore, within a group of fish, estimates of ṀO2,max determined during swimming are poorly correlated with estimates determined during recovery from chasing (i.e. an individual's ṀO2,max is not repeatable across methods). One explanation for the lack of agreement is that these methods measure different physiological states, each with their own behavioural, anatomical and biochemical determinants. We propose that these methods are not directly interchangeable but, rather, each is suited to address different questions in fish biology. We suggest that researchers select the method that reflects the biological contexts of their study, and we advocate for the use of accurate terminology that acknowledges the technique used to elevate ṀO2 (e.g. peak ṀO2,swim or peak ṀO2,recovery). If the study's objective is to estimate the ‘true’ ṀO2,max of an individual or species, we recommend that pilot studies compare methods, preferably using repeated-measures designs. We hope that these recommendations contribute new insights into the causes and consequences of variation in ṀO2,max within and among fish species.

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Section: Improving Methods To Measure Peak ṁ Omentioning

confidence: 82%

Estimating maximum oxygen uptake of fishes during swimming and following exhaustive chase – different results, biological bases and applications

Rees,

Reemeyer,

Binning

et al. 2024

Journal of Experimental Biology

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“…However, if one of the ‘calcSMR’ methods is selected to determine SMR (Chabot et al , 2016b), a user should first extract all slopes to be able to plot linear models, and then apply a ‘calcSMR’ method (see Case Study 2 and File S2). Note that an exponential decrease of oxygen content over long measurement periods might be caused by chamber leaking or poor water circulation in a respirometry system even with high r 2 value (Svendsen et al , 2016b; Norin and Clark, 2017). Would this be the case, those data should be omitted from further analyses.…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, users can define a threshold r 2 value (default value of 0.95), the parameter used to detect mechanical problems in a respirometry system (e.g. chamber leaking and poor water flow) or spontaneous animal activity during SMR measurements (but see Clark et al , 2013; Norin and Clark, 2017; Zhang and Gilbert, 2017).…”

Section: Methodsmentioning

confidence: 99%

FishResp: R package and GUI application for analysis of aquatic respirometry data

Morozov

McCairns

Merilä

2019

Conservation Physiology

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FishResp: R Package and GUI Application for Analysis of Aquatic Respirometry Data

Morozov

McCairns

Merilä

2018

Preprint

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32Intermittent-flow respirometry is widely used to measure oxygen uptake rates and subsequently 33 estimate aerobic metabolic rates of aquatic animals. However, the lack of a standard quality-control 34 software to detect technical problems represents a potential impediment to comparisons across studies. 35Here, we introduce 'FishResp', a versatile R package and its graphical implementation for quality-36 control and filtering of raw respirometry data. Our goal is to provide a straightforward, cross-platform 37and free software to help improve the quality and comparability of metabolic rate estimates for 38 reducing methodological fragmentation in the field of aquatic respirometry. FishResp accepts data from 39 various respirometry systems, allows users to detect potential mechanical problems which can occur 40 during oxygen uptake measurements (e.g. chamber leaking, poor water circulation), and offers six 41 options to correct raw data for microbial oxygen consumption. The software performs filtering of raw 42 data based on user criteria, and produces accurate and unbiased estimates of absolute and mass-specific 43 metabolic rates. Using data from three-spined sticklebacks (Gasterosteus aculeatus) and Trinidadian 44 guppies (Poecilia reticulata), we demonstrate the virtues of FishResp, highlighting the importance of 45 detecting mechanical problems and correcting measurements for background respiration. 46

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Reply to Zhang & Gilbert (2017): Comment on ‘Measurement and relevance of maximum metabolic rate in fishes by Norin & Clark (2016)’

Cited by 4 publications

References 19 publications

Estimating maximum oxygen uptake of fishes during swimming and following exhaustive chase – different results, biological bases and applications

Estimating maximum oxygen uptake of fishes during swimming and following exhaustive chase – different results, biological bases and applications

FishResp: R package and GUI application for analysis of aquatic respirometry data

FishResp: R Package and GUI Application for Analysis of Aquatic Respirometry Data

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