Report of the Second Equine Leucocyte Antigen Workshop, Squaw Valley, California, July 1995

Lunn, D.P.; Holmes, Mark A.; Antczak, Douglas F.; Agerwal, N; Baker, John; Bendali-Ahcene, S.; Blanchard-Channell, M.; Byrne, Katherine; Cannizzo, Karen; Davis, William C.; Hamilton, Mary Jo; Hannant, D.; Kondo, Takashi; Kydd, Julia H.; Monier, M.C; Moore, Peter F.; O’Neil, Terri E.; Schram, Brian R.; Sheoran, Abhineet S.; Stott, Jeffrey L; Sugiura, Takeo; Vagnoni, Karen E.

doi:10.1016/s0165-2427(97)00160-8

Cited by 124 publications

(41 citation statements)

References 51 publications

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“…37) and in the past 6 years have also been studied using mAbs (38,39). In these studies, five IgG subclasses of the horse were described, designated IgGa, IgGb, IgGc, IgG(T), and IgG(B), with the last initially named equine aggregating Ig.…”

Section: Discussionmentioning

confidence: 99%

The Complete Map of the Ig Heavy Chain Constant Gene Region Reveals Evidence for Seven IgG Isotypes and for IgD in the Horse

Wagner

Miller

Lear

et al. 2004

The Journal of Immunology

100

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This report contains the first map of the complete Ig H chain constant (IGHC) gene region of the horse (Equus caballus), represented by 34 overlapping clones from a new bacterial artificial chromosome library. The different bacterial artificial chromosome inserts containing IGHC genes were identified and arranged by hybridization using overgo probes specific for individual equine IGHC genes. The analysis of these IGHC clones identified two previously undetected IGHC genes of the horse. The newly found IGHG7 gene, which has a high homology to the equine IGHG4 gene, is located between the IGHG3 and IGHG4 genes. The high degree of conservation shared between the nucleotide sequences of the IGHG7 and IGHG4 genes is unusual for the IGHG genes of the horse and suggests that these two genes duplicated most recently during evolution of the equine IGHG genes. Second, we present the genomic nucleotide sequence of the equine IGHD gene, which is located downstream of the IGHM gene. Both the IGHG7 and IGHD genes were found to be expressed at the mRNA level. The order of the 11 IGHC genes in the IGH-locus of the horse was determined to be 5′-M-D-G1-G2-G3-G7-G4-G6-G5-E-A-3′, confirming previous studies using λ phage clones, with the exception that the IGHG5 gene was found to be the most downstream-located IGHG gene. Fluorescence in situ hybridization was used to localize the IGHC region to Equus caballus (ECA) 24qter, the horse chromosome corresponding to human chromosome 14, where the human IGH locus is found.

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Section: Discussionmentioning

confidence: 99%

The Complete Map of the Ig Heavy Chain Constant Gene Region Reveals Evidence for Seven IgG Isotypes and for IgD in the Horse

Wagner

Miller

Lear

et al. 2004

The Journal of Immunology

100

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“…The proteins were either stained in the gel using Coomassie Brilliant Blue or blotted to PVDF membranes (Bio-Rad, Hercules, CA, USA). The membranes were incubated with the following monoclonal antibodies to equine IgG: anti-IgGa (CVS45), anti-IgGb (CVS39), anti-IgGc (CVS52), and anti-IgG(T) (CVS40) [16,26]. The CVS antibodies were kindly provided by Dr P. Lunn (Colorado State University, Fort Collins, CO, USA).…”

Section: Sds-page and Western Blottingmentioning

confidence: 99%

IgE and IgG antibodies in skin allergy of the horse

et al. 2006

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-In horses, allergies have been characterized by clinical signs and/or intradermal (i.d.) allergen testing. Our aim was to find the first direct evidence that immunoglobulin E (IgE) mediates equine allergy. In addition, we tested the hypothesis that immediate skin reactions in horses can also be mediated by IgG. Anti-IgE affinity columns were used to purify IgE from serum of one healthy horse and three horses affected with summer eczema, an allergic dermatitis which is believed to be induced by Culicoides midges. A modified Prausnitz-Küstner experiment was performed in four clinical healthy horses by i.d. injection of the purified serum IgE antibodies. The following day, Culicoides allergen was injected at the same sites. Skin reactions were not observed in response to allergen alone, and in two horses after stimulation at any previous IgE injection site. However, the other two horses showed an immediate skin reaction at the previous injection sites of IgE obtained from allergic horses. In addition, purified monoclonal antibodies to various equine immunoglobulin isotypes were injected i.d. into six healthy horses. Immediate skin reactions were observed in response to anti-IgE (6/6 horses) and anti-IgG(T) injections (5/6 horses). The specificities of both antibodies for IgE and IgG(T), respectively, were confirmed by enzyme linked immunosorbent assays. The results provide the first direct evidence that IgE mediates classical Type-I allergy in horses and plays a major role in the pathogenesis of summer eczema. The data also suggest that IgG(T) can bind to skin mast cells and might contribute to clinical allergy.horse / allergy / summer eczema / IgE / IgG

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“…IgGa and IgGb antibodies are cytophilic and capable of both complement activation and opsonization, while IgG(T) and IgGc are noncytophilic and may block the protective effects of cytophilic antibodies by competitive binding to antigen (15,17). In this study, the kinetics of the most prevalent IgG isotypes in horse serum, the IgGa, IgGb, and IgG(T) antibodies (22), were characterized during acute and chronic B. equi infection; the potential use of these isotypes as immunological markers for infection control and vaccine development is discussed.…”

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confidence: 99%

Development of Specific Immunoglobulin Ga (IgGa) and IgGb Antibodies Correlates with Control of Parasitemia in Babesia equi Infection

Cunha

McGuire

Kappmeyer

et al. 2006

Clin Vaccine Immunol

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Report of the Second Equine Leucocyte Antigen Workshop, Squaw Valley, California, July 1995

Cited by 124 publications

References 51 publications

The Complete Map of the Ig Heavy Chain Constant Gene Region Reveals Evidence for Seven IgG Isotypes and for IgD in the Horse

The Complete Map of the Ig Heavy Chain Constant Gene Region Reveals Evidence for Seven IgG Isotypes and for IgD in the Horse

IgE and IgG antibodies in skin allergy of the horse

Development of Specific Immunoglobulin Ga (IgGa) and IgGb Antibodies Correlates with Control of Parasitemia in Babesia equi Infection

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