“…The CHC13 fraction from the hydrolysis was purified by Si gel cc with CHCl3-MeOH (95:5) as eluent. The fractions (1-7) containing glycyr- cal methods have been employed for the determination of glycyrrhizin and glycyrrhetinic acid in plant material and pharmaceutical and food preparations, such as gc (17), hplc (18,19), tic (20), and hptlc (21). In the present study, an hplc method was used, with the solvent system (16) being Me0H2-H20 (80:20), S',0.6 ml/min.…”