Report on the Forensic Conference at Manchester Metropolitan University

Walters, Philippa

doi:10.53841/bpstalk.2007.1.57.41

Cited by 1 publication

(1 citation statement)

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“…Aging is a complex phenomenon marked by a gradual decline in physiological function and behavioral capacity. This natural process emerges from the gradual accumulation of molecular and cellular damage during an individual’s lifetime [5, 6]. The accumulation of damage might ultimately surpass the body’s capacity to repair and eliminate impaired materials, resulting in an imbalance that hampers the optimal functioning of different body systems.…”

Section: Introductionmentioning

confidence: 99%

Preservation of an Aging-Associated Mitochondrial Signature in Advanced Human Neuronal Models

Varghese,

Szabo,

Cader

et al. 2024

Preprint

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This study investigated whether induced pluripotent stem cell-derived neurons (iPSCsNs) and directly converted neurons (iNs) generated from the same cells of origin (human fibroblasts) represent aging-related characteristics on mitochondrial levels. There is still uncertainty regarding the potential for rejuvenation or preservation of an aging-associated donor signature in aged iPSCsNs upon transition through pluripotent states, while direct conversion retains the aging-associated mitochondrial impairments. Surprisingly, both aged neuronal models exhibited age-associated donor phenotypes, including decreased ATP, mitochondrial membrane potential, mitochondrial respiration, NAD+/NADH ratio, and increased radical levels and mitochondrial mass. Besides, a fragmented mitochondrial network was observed in both aged neuronal models. However, unlike aged iNs, aged iPSCsNs did not show a metabolic shift towards anaerobic glycolysis to compensate for the energy deficit. Moreover, the mRNA expression profile significantly differed between aged iPSCsNs and aged iNs. Our study indicates that aged iPSCsNs may experience rejuvenation in certain parameters, such as transcriptomics and the aging-associated glycolytic shift. Nevertheless, aged iPSCsNs can be a valuable tool for studying neuronal aging of mitochondrial parameters in vitro alongside aged iNs.

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Section: Introductionmentioning

confidence: 99%