We determined measurement properties of the Sedentary Time and Activity Reporting Questionnaire (STAR-Q), which was designed to estimate past-month activity energy expenditure (AEE). STAR-Q validity and reliability were assessed in 102 adults in Alberta, Canada (2009, who completed 14-day doubly labeled water (DLW) protocols, 7-day activity diaries on day 15, and the STAR-Q on day 14 and again at 3 and 6 months. Three-month reliability was substantial for total energy expenditure (TEE) and AEE (intraclass correlation coefficients of 0.84 and 0.73, respectively), while 6-month reliability was moderate. STAR-Q-derived TEE and AEE were moderately correlated with DLW estimates (Spearman's ρ s of 0.53 and 0.40, respectively; P < 0.001), and on average, the STAR-Q overestimated TEE and AEE (median differences were 367 kcal/day and 293 kcal/day, respectively). Body mass index-, age-, sex-, and season-adjusted concordance correlation coefficients (CCCs) were 0.24 (95% confidence interval (CI): 0.07, 0.36) and 0.21 (95% CI: 0.11, 0.32) for STAR-Q-derived versus DLW-derived TEE and AEE, respectively. Agreement between the diaries and STAR-Q (metabolic equivalent-hours/day) was strongest for occupational sedentary time (adjusted CCC = 0.76, 95% CI: 0.64, 0.85) and overall strenuous activity (adjusted CCC = 0.64, 95% CI: 0.49, 0.76). The STAR-Q demonstrated substantial validity for estimating occupational sedentary time and strenuous activity and fair validity for ranking individuals by AEE. motor activity; physical activity; reproducibility of results; sedentary lifestyle; questionnaires; validation studies Abbreviations: AEE, activity energy expenditure; CCC, concordance correlation coefficient; DLW, doubly labeled water; ICC, intraclass correlation coefficient; MET, metabolic equivalent of task; STAR-Q, Sedentary Time and Activity Reporting Questionnaire; TEE, total energy expenditure.As the need for comprehensive assessments of daily physical activity has grown, the limitations of existing methods have become apparent (1, 2). Many physical activity questionnaires have been developed and validated, but most are not well suited for the study of new behavioral parameters of interest, such as activity energy expenditure (AEE). In a review of physical activity questionnaires that were validated against doubly labeled water (DLW)-the gold standard for estimating total energy expenditure (TEE) in free-living individuals-we reported that overall, questionnaire performance in estimating AEE was poor when compared with DLW (3). However, none of the questionnaires were originally designed to estimate AEE.While objective measures continue to evolve in precision and accuracy, they are limited as "stand-alone" measures when contextual information pertaining to habitual behavior is desired (4). It is generally agreed that, for at least the foreseeable future, there will continue to be a need for logistically feasible, cost-effective tools with known validity that can assess a broad range of habitual behaviors in large populations (4, ...