Reporting effective extraction methodology and chemical characterization of bioactive components of under explored Platycladus orientalis (L.) Franco from semi-arid climate

Abstract

The bioactive ingredients of many essential oils are complex and difficult to reproduce synthetically. In present study, the influence of extraction method on relative extraction of bioactive compounds of essential oil of Platycladus orientalis (L.) Franco (Thuja orientalis L. is a synonym of it) leaves gathered from semi-arid climate was studied. A higher yield of essential oil was obtained by hydrodistillation (0.1%) as compared to steam distillation (0.07%) under newly optimized conditions. Initial analysis of these oils by TLC showed the presence of different polarity groups ranging from non-polar terpene hydrocarbons to polar terpenoid alcohols. GC-MS analysis revealed that major component of hydrodistilled essential oil of Platycladus orientalis (L.) Franco leaves was α-pinene (17.83%) which is a bioactive monoterpene whereas steam distilled essential oil contained α-cedrol (12.44%) as a major component. The results obtained in the present study emphasize that suitable extraction technique should be used to obtain component of interest. Also, both extraction techniques should be used to study complete chemical composition of essential oil from a particular plant.

Extraction Yield, Chemical Composition, Preliminary Toxicity of Bignonia nocturna (Bignoniaceae) Essential Oil and in Silico Evaluation of the Interaction

“…In general, extraction by HD was more efficient than that by SD. HD was also found to be a more efficient process for extracting essential oils from Platycladus orientalis , [24] and Origanum majorana [25] . However, for Rosmarinus officinalis , [26] and Thymus vulgaris , [27] HD was not the most efficient method.…”