The present research aimed to analyze the relation of Homophobia with anxiety symptoms; wherein the specific objectives were to characterize the profile of the participants; analyze anxiety in people with and without interest in homoaffective relationships; compare the level of anxiety in victims and non-victims of homophobia. For this, a descriptive field research, almost experimental design and quantitative in nature was carried out. Regarding data collection, a sociodemographic form was used; as well as a self-identification questionnaire as a victim of homophobia; and, finally, the Anx-iety Inventory of the “Mind Over Mood”. A total of 241 individuals participated in this research, mostly cisgender women. According to the results, it was identified that people who have an interest on homoaffective relationships have a significantly higher level of anxiety than those who do not (t (239) = 3,143; p < 0,05); it was also found that people who have already suffered homophobia, have a significantly higher level of anxiety than those who have not (t (239) = 2,641; p < 0,05). In conclusion, the study is conducive to mental health professionals, especially the psychologist, to look at the process of investigation and care of the LGB patient (lesbian, gay, bisexual, respective-ly), understanding homophobia as the problem and not the human sexuality variable.