Repression of anthocyanin biosynthesis by R3-MYB transcription factors in lily (Lilium spp.)

Sakai, Masaaki; Yamagishi, Masumi; Matsuyama, Kohei

doi:10.1007/s00299-019-02391-4

Cited by 50 publications

(32 citation statements)

References 69 publications

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“…Hence, we further verified that PMPGs involved in initial phenylpropanoid metabolic pathway and LBGs involved in late anthocyanin biosynthesis are much more sensitive than EBGs to low temperature treatment. Based on extensive studies of plants, an increasing number of evidence demonstrated that anthocyanin biosynthesis is usually activated by MBW complex in transcriptional control of LBGs, and loss-of-function mutant in these genes often lead to the colorless phenotype [4,56]. In addition, the mechanism of different key MYB factors controlling anthocyanin biosynthesis may be independent or synergetic to regulate anthocyanin accumulation in a plant, and members of them show a tissue-specific character.…”

Section: Discussionmentioning

confidence: 99%

Low Temperature Promotes Anthocyanin Biosynthesis and Related Gene Expression in the Seedlings of Purple Head Chinese Cabbage (Brassica rapa L.)

Ren

Zhao

et al. 2020

Genes

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To elucidate the effect of low temperature on anthocyanin biosynthesis in purple head Chinese cabbage, we analyzed anthocyanin accumulation and related gene expression in the seedlings of purple head Chinese cabbage, white head parent Chinese cabbage, and its purple male parent under a normal 25 °C temperature and a low 12 °C temperature. Anthocyanin accumulation in purple lines was strongly induced by low temperature, and the total anthocyanin content of seedlings was significantly enhanced. In addition, nearly all phenylpropanoid metabolic pathway genes (PMPGs) were down-regulated, some early biosynthesis genes (EBGs) were up-regulated, and nearly all late biosynthesis genes (LBGs) directly involved in anthocyanin biosynthesis showed higher expression levels in purple lines after low-temperature induction. Interestingly, a R2R3-MYB transcription factor (TF) gene ‘BrMYB2’ and a basic-helix-loop-helix (bHLH) regulatory gene ‘BrTT8’ were highly up-regulated in purple lines after low temperature induction, and two negative regulatory genes ‘BrMYBL2.1’ and ‘BrLBD38.2’ were up-regulated in the white line. BrMYB2 and BrTT8 may play important roles in co-activating the anthocyanin structural genes in purple head Chinese cabbage after low-temperature induction, whereas down-regulation of BrMYB2 and up-regulation of some negative regulators might be responsible for white head phenotype formation. Data presented here provide new understanding into the anthocyanin biosynthesis mechanism during low temperature exposure in Brassica crops.

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Section: Discussionmentioning

confidence: 99%

Low Temperature Promotes Anthocyanin Biosynthesis and Related Gene Expression in the Seedlings of Purple Head Chinese Cabbage (Brassica rapa L.)

Ren

Zhao

et al. 2020

Genes

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“…Other R3‐MYBs derived from subgroup 4 MYBs through N‐terminal truncations have also been reported in the past few years, including IlMYBL1 from Iochroma loxense (Gates et al ., 2018) and LhR3MYB1/2 from hybrid lilies ( Lilium spp. ; Sakai et al ., 2019). These R3‐MYBs contain an EAR motif but not the ‘TLLLFR’ motif, indicating different origins than AtMYBL2, and they appear dispersed on phylogenetic trees in a species‐specific fashion (Gates et al ., 2018), indicating their independent, lineage‐specific origins.…”

Section: Developmental Cuesmentioning

confidence: 99%

Repressors of anthocyanin biosynthesis

2021

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Anthocyanins play a variety of adaptive roles in both vegetative tissues and reproductive organs of plants. The broad functionality of these compounds requires sophisticated regulation of the anthocyanin biosynthesis pathway to allow proper localization, timing, and optimal intensity of pigment deposition. While it is well-established that the committed steps of anthocyanin biosynthesis are activated by a highly conserved MYB-bHLH-WDR (MBW) protein complex in virtually all flowering plants, anthocyanin repression seems to be achieved by a wide variety of protein and small RNA families that function in different tissue types and in response to different developmental, environmental, and hormonal cues. In this review, we survey recent progress in the identification of anthocyanin repressors and the characterization of their molecular mechanisms. We find that these seemingly very different repression modules act through a remarkably similar logic, the so-called "double negative logic". Much of the double-negative regulation of anthocyanin production involves signal-induced degradation or sequestration of the repressors from the MBW protein complex. We discuss the functional and evolutionary advantages of this logic design compared with simple or sequential positive regulation. These advantages provide a plausible explanation to why plants have evolved so many anthocyanin repressors.

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“…In addition, MiMYB1 has been shown to interact with MibHLH2 and MiWDR1 to activate the transcription of the anthocyanin biosynthetic genes MiF3 H, MiDFR, and MiANS in Matthiola incana owers [43]. Moreover, some R2R3-MYB repressors have also been identi ed in plants in recent years, including PtrMYB182 and PtrMYB57 from poplar, PpMYB17-20 from peach, MdHB1, MdMYB16, and MdMYB15L from apple, NtMYB2 from Chinese narcissus, and CmMYB#7 from chrysanthemum [44][45][46][47][48][49][50][51]. In apple, MdHB1 have been supposed to constrain MdMYB10, MdbHLH3, and MdTTG1 to the cytoplasm, and then represses the transcription of MdDFR and MdUFGT indirectly and resulted in reduced anthocyanin biosynthesis [47].…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analyses reveal genes related to pigmentation in the petals of a flower color variation cultivar of Rhododendron obtusum

Sun¹,

He²,

Guo³

et al. 2021

Preprint

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Rhododendron is an important woody ornamental plant and breeding varieties with different colors is vital research goal. In this study, a flower color variation cultivar ‘Yanzhi Mi’ (pink petals) and the wild-type (WT) cultivar ‘Dayuanyangjin’ (white petals with pink stripes) were used as research objects, the pigment and transcriptome of their petals during different flower development (stage S1, S2, S3, S4 and S5) were analyzed and compared. The results showed that the derivatives of cyanidin, peonidin and pelargonidin may be responsible for the pink of mutant petals and S2 stage (buds showing color at the top but with the scales still present) was the key stage of flower color formation of mutant. In total, 412,910 transcripts and 2,780 differentially expressed genes (DEGs) were identified in pairwise comparisons of WT and mutant petals. GO and KEGG enrichment analyses of the DEGs showed that the ‘DNA-binding transcription factor activity’, ‘Flavonoid biosynthesis’ and ‘Phenylpropanoid biosynthesis’ were more active in mutant petals. Early anthocyanin pathway candidate DEGs (CHS3-CHS6, CHI, F3Hs and F3’H) were strongly correlated and up-regulated expression in mutant petals than in WT petals at S2 stage. These genes may be the key structural genes for the pink coloration of mutant petals. In the petals of mutant, two R2R3-MYB unigene (TRINITY_DN59015_c3_g2 and TRINITY_DN49281_c1_g6) were identified as repressors involved in anthocyanin regulation and were significantly down-regulated at S2 stage. This study shed light on the biochemistry and genetic mechanisms underlying the flower coloration in two Rhododendron obtusum cultivars.

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Repression of anthocyanin biosynthesis by R3-MYB transcription factors in lily (Lilium spp.)

Cited by 50 publications

References 69 publications

Low Temperature Promotes Anthocyanin Biosynthesis and Related Gene Expression in the Seedlings of Purple Head Chinese Cabbage (Brassica rapa L.)

Low Temperature Promotes Anthocyanin Biosynthesis and Related Gene Expression in the Seedlings of Purple Head Chinese Cabbage (Brassica rapa L.)

Repressors of anthocyanin biosynthesis

Comparative transcriptome analyses reveal genes related to pigmentation in the petals of a flower color variation cultivar of Rhododendron obtusum

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