Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles

Zhou, Lixiao; Yu, Ziying; Xia, Yinyin; Cheng, Shuqun; Gao, Jieying; Sun, Wei; Jiang, Xuejun; Zhang, Jun; Mao, Lejiao; Qin, Xia; Zou, Zhen; Qiu, Jingfu; Chen, Chengzhi

doi:10.1016/j.envint.2022.107220

Cited by 44 publications

(26 citation statements)

References 76 publications

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“…Therefore, environmental MPs degrade to smaller NPs after aging may pose even more significant health risks to humans, and its hazards should be paid more attention to. Due to the widespread concern about male reproductive health, the male reproductive toxicity of NPs in mammals has also been investigated 29,37,38 . However, the mechanism of male reproductive system damage caused by NPs is still unclear.…”

Section: Discussionmentioning

confidence: 99%

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Integrated fecal microbiome and metabolome analysis explore the link between polystyrene nanoplastics exposure and male reproductive toxicity in mice

Liu

Han

et al. 2023

Environmental Toxicology

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Microplastics (MPs) and nanoplastics (NPs) are novel environmental pollutants that are ubiquitous in the environment and everyday life. NPs can easily enter the tissues and have more significant potential health risks due to their smaller diameter. Previous studies have shown that NPs can induce male reproductive toxicity, but the detailed mechanisms remain uncertain. In this study, intragastric administration treated mice with polystyrene NPs (PS‐NPs, 50, and 90 nm) at 3 and 15 mg/mL/day doses for 30 days. Then, the fresh fecal samples were collected from those mice that the exposure doses of 50 nm PS‐NPs at 3 mg/mL/day and 90 nm at 15 mg/mL/day for subsequent investigations of 16S rRNA and metabolomics according to significant toxicological effects (Sperm number, viability, abnormality, and testosterone level). The conjoint analysis findings indicated that PS‐NPs disrupted the homeostasis of the gut microbiota, metabolism, and male reproduction, suggesting that abnormal gut microbiota‐metabolite pathways may be important in PS‐NPs‐induced male reproductive toxicity. Meanwhile, the common differential metabolites such as 4‐deoxy‐Erythronic acid, 8‐iso‐15‐keto‐PGE2, apo‐10′‐violaxanthin, beta‐D‐glucosamine, isokobusone, oleamide, oxoadipic acid, sphingosine induced by 50 and 90 nm PS‐NPs might be used as biomarkers to explore PS‐NPs‐induced male reproductive toxicity. In addition, this study systematically demonstrated that nano‐scale PS‐NPs induced male reproductive toxicity via the crosstalk of gut microbiota and metabolites. It also provided valuable insights into the toxicity of PS‐NPs, which was conducive to reproductive health risk assessment for public health prevention and treatment.

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Section: Discussionmentioning

confidence: 99%

“…Due to the widespread concern about male reproductive health, the male reproductive toxicity of NPs in mammals has also been investigated. 29,37,38 However, the mechanism of male reproductive system damage caused by NPs is still unclear. The gut microbiota is important in modulating metabolism and maintaining the host's health.…”

Section: Discussionmentioning

confidence: 99%

Integrated fecal microbiome and metabolome analysis explore the link between polystyrene nanoplastics exposure and male reproductive toxicity in mice

Liu

Han

et al. 2023

Environmental Toxicology

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“…The same idea applies to the study of MPs and NPs in rodent reproductive organs. The accumulation of MPs or NPs in mouse reproductive organs has only been reported in studies with particle diameters less than 10 μm, whereas it has not been reported in studies with particle diameters greater than this criterion (Jin et al, 2021; Liang et al, 2021; Wei et al, 2022; Zhou et al, 2022). For MPs with particle diameters ranging from 1 to 10 μm, the accumulation in mouse reproductive organs has been reported as controversial.…”

Section: Accumulation Of Mps and Nps In The Reproductive Tissues And ...mentioning

confidence: 99%

“…According to the comments of Lenz et al (2016), the concentration of MPs in water in the natural environment ranges from 1 pg/L to 15 μg/L, and since the daily water intake of common mouse strains ranges from 5.7 ± 0.2 ml/30 g body weight to 11.4 ± 0.5 ml/30 g body weight (Bachmanov et al, 2002), it can be deduced that the daily intake of MPs in mice in the natural environment is less than 0.171 ± 0.075 μg/30 g body weight. It indicates that in some animal model‐based experiments, the daily MP intake of mice (usually ranging from 6 μg/30 g body weight to 1,000 μg/30 g body weight) is far greater than the natural level (Jin et al, 2021; Liang et al, 2021; Wei et al, 2022; Zhou et al, 2022).…”

Section: Accumulation Of Mps and Nps In The Reproductive Tissues And ...mentioning

confidence: 99%

“…These studies exposed rodents to MPs or NPs through the digestive tract by gavage administration or direct drinking of drinking water containing MPs or NPs. Abnormalities included the absence of acrosome (Jin et al, 2021), small‐headed (Jin et al, 2021; Zhou et al, 2022), acephalia (Amereh et al, 2020; Hou, Wang, et al, 2021; Jin et al, 2021), double‐headed (Amereh et al, 2020; Zhou et al, 2022), cervical folding (Jin et al, 2021; Zhou et al, 2022), tailless (Amereh et al, 2020; Jin et al, 2021), double‐tailed (Amereh et al, 2020; Hou, Wang, et al, 2021), tail‐curling (Amereh et al, 2020), and cervical swelling (Hou, Wang, et al, 2021). Poor fluidity (Amereh et al, 2020; Liu, Hou, Zhang, et al, 2022), decreased mobility (Amereh et al, 2020; Ijaz et al, 2021; Jin et al, 2022; Li, Wang, et al, 2021; Zhou et al, 2022), and lower quantity (Amereh et al, 2020; Hou, Wang, et al, 2021; Ijaz et al, 2021; Li, Wang, et al, 2021; Liu, Hou, Zhang, et al, 2022; Wei et al, 2022; Xie et al, 2020; Zhou et al, 2022) suggested that the sperm motility of mice or rats may be reduced after exposure to MPs or NPs.…”

Section: Fertility Toxicity Of Mps and Nps To Rodent And The Mechanismsmentioning

confidence: 99%

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The reproductive and transgenerational toxicity of microplastics and nanoplastics: A threat to mammalian fertility in both sexes

Yin

2023

J of Applied Toxicology

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Microplastics (MPs) and nanoplastics (NPs) are extensively distributed in the environment. However, a comprehensive review and in‐depth discussion on the effects of MPs and NPs to reproductive capacity and transgenerational toxicity on mammals, especially on humans, is lacked. It is suggested that microplastics and nanoplastics could accumulate in mammalian reproductive organs and exert toxic effects on the reproductive system for both sexes. For males, the damage of microplastics consists of abnormal testicular and sperm structure, decreased sperm vitality, and endocrine disruption, which were caused by oxidative stress, inflammation, apoptosis of testicular cells, autophagy, abnormal cytoskeleton, and abnormal hypothalamic‐pituitary‐testicular axis. For females, the damage of microplastics includes abnormal ovary and uterus structure and endocrine disruption, which were caused by oxidative stress, inflammation, granulosa cell apoptosis, hypothalamic‐pituitary‐ovary axis abnormalities, and tissue fibrosis. For transgenerational toxicity, premature mortality existed in the rodent offspring after maternal exposure to microplastics. Among the surviving offspring, metabolic disorders, reproductive dysfunction, immune, neurodevelopmental, and cognitive disorders were detected, and these events directly correlated with transgenerational translocation of MPs and NPs. Studies on human‐derived cells or organoids demonstrated that transgenerational toxicity studies for both sexes are yet in the phase of exploring suitable experimental models, and more detailed research on the threat of MPs and NPs to human fertility is still urgently needed. Further studies will help assess the MPs and NPs threat to public fertility and reproductive health risks.

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Oral exposure to polystyrene nanoplastics altered the hypothalamic–pituitary–testicular axis role in hormonal regulation, inducing reproductive toxicity in albino rats

Ebrahim,

El‐Dakdoky,

El‐Shafiey

et al. 2024

Birth Defects Research

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BackgroundNanoplastics can be considered a novel contaminant for the environment because of their extensive applications in modern society, which represents a possible threat to humans. Nevertheless, the negative effect of polystyrene nanoplastics (PS‐NPs) on male reproduction, fertility, and progeny outcomes is not well known. Thus, the aim of the present work was to calculate the median lethal dose (LD50) and investigate the consequences of exposure to PS‐NPs (25 nm) on male reproductive toxicity.MethodsThis investigation first determined the LD50 of PS‐NPs in male Wistar rats, and then in a formal study, 24 rats were distributed into three groups (n = 8): the control group; the low‐dose group (3 mg/kg bw); and the high‐dose group (10 mg/kg bw) of PS‐NPs administered orally for 60 days. On the 50th day of administration, the fertility test was conducted.ResultsThe LD50 was determined to be 2500 mg/kg. PS‐NP administration induced significant alternations, mainly indicating mortality in the high‐dose group, a significant elevation in body weight gain, declined sperm quality parameters, altered reproductive hormonal levels, thyroid endocrine disruption, an alternation of the normal histo‐architecture and the histo‐morphometric analysis of the testes, and impaired male fertility.ConclusionAltogether, the current findings provide novel perspectives on PS‐NP general toxicity with specific reference to male reproductive toxicity.

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Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles

Cited by 44 publications

References 76 publications

Integrated fecal microbiome and metabolome analysis explore the link between polystyrene nanoplastics exposure and male reproductive toxicity in mice

Integrated fecal microbiome and metabolome analysis explore the link between polystyrene nanoplastics exposure and male reproductive toxicity in mice

The reproductive and transgenerational toxicity of microplastics and nanoplastics: A threat to mammalian fertility in both sexes

Oral exposure to polystyrene nanoplastics altered the hypothalamic–pituitary–testicular axis role in hormonal regulation, inducing reproductive toxicity in albino rats

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