Repression of Seed Maturation Genes by a Trihelix Transcriptional Repressor in<i>Arabidopsis</i>Seedlings

Gao, Ming‐Jun; Lydiate, Derek J.; Li, Xiang; Lui, Helen; Gjetvaj, Branimir; Hegedus, Dwayne D.; Rozwadowski, Kevin

doi:10.1105/tpc.108.061309

Cited by 134 publications

(158 citation statements)

References 81 publications

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“…An equal amount of protein (10 µg) was loaded in each lane and subjected to SDS-PAGE followed by either Coomassie brilliant blue staining (left) or immunoblot analysis with monoclonal anti-12S cruciferin (CRC) antibody (right) as described previously. 1 …”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

“…ASIL1 expression is dynamically regulated during seed development and relatively high levels of ASIL1 transcript accumulated during early-and mid-embryogenesis. 1 Recently, ASIL1 was found to be involved in the temporal regulation of embryo maturation in Arabidopsis in a partially redundant manner. 2 To examine whether ASIL1 is involved in the regulation of seed filling, we compared changes in the expression of seed-specific genes in wild-type and asil1-1 mutant plants 1 by real-time RT-PCR analysis with RNA isolated from siliques corresponding to five different stages of silique development: early-embryogenesis I (1-3 DPA), early-embryogenesis II (4-6 DPA), mid-embryogenesis (7-9 DPA), late-embryogenesis I (10-12 DPA) and late-embryogenesis II (13- RESEARCH PAPER mature and completely dry after 15 DPA.…”

Section: Asil1 Is Required For Proper Timing Of Seed Filling In Arabimentioning

confidence: 99%

“…Consistent with this, asil1 seedlings accumulate 2S albumin and oil comprised of fatty acids similar to that of seed-derived lipid. 1 Flowering plants require proper timing for control of their development. This requires the correct timing of cell division and a change in the balance of expression of numerous genes.…”

Section: Asil1 Is Required For Proper Timing Of Seed Filling In Arabimentioning

confidence: 99%

“…Total RNA was isolated from siliques of wild type (Col-0) and asil1-1 corresponding to five developmental stages as indicated (DPA) and subjected to real-time RT-PCR analysis with ACT2 mRNA as an internal reference and gene-specific primers as previously described. 1 The mean and SD from qRT-PCR analysis were determined from three biological replicates of either Col-0 or asil1-1.…”

Section: Asil1 Is Required For Proper Timing Of Seed Filling In Arabimentioning

confidence: 99%

“…Mutation of ASIL1 derepressed expression of a subset of embryonic genes resulting in accumulation of 2S albumin and embryo-specific lipids in leaves. 1 It was recently reported that mutation of ASIL1 led to early embryo development in Arabidopsis. 2 In this study, we demonstrated that ASIL1 acts as a temporal regulator of seed filling.…”

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ASIL1 is required for proper timing of seed filling in Arabidopsis

Gao

Lui

et al. 2011

Plant Signaling & Behavior

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Seeds offer plants a unique opportunity to suspend their life cycles in a desiccated state. This enables them to endure adverse environmental conditions and then resume growth using endogenous storage reserves when more favorable conditions develop. Seed formation is an intricate genetically programmed process that is correlated with changes in metabolite levels and is regulated by a complex signaling network mediated mainly by hormone and sugar levels. 3,4 Seed development can be divided into two stages, embryo morphogenesis and maturation. The hallmarks of maturation include storage compound accumulation, acquisition of desiccation tolerance, growth arrest and entry into a dormancy period that is broken upon germination. 5 In Arabidopsis, three members of the B3 family of transcription factors, LEAFY COTYLEDON (LEC) 2, ABSCISIC ACID-INSENTITIVE 3 (ABI3) and FUSCA 3 (FUS3) as well as a fourth regulator, a HAP3 subunit of the CCAAT-box binding transcription factor (CBF) LEC1, are master regulators of seed maturation processes. A redundant gene regulatory network linking these regulators was elucidated by examining the expression of ABI3, FUS3 and LEC2 in abi3, fus3, lec1 and lec2 single, double and triple mutants. 6 In combination with ABA, GA, auxin and sugar signaling, this regulatory network governs most seed-specific maturation traits in a partially redundant manner. 6 The regulatory networks controlling the seed maturation program in flowering plants are repressed prior to germination so that seed storage reserves do not accumulate during vegetativeIn flowering plants, seed development and seed filling are intricate genetically programmed processes that correlate with changes in metabolite levels and that are spatially and temporally regulated by a complex signaling network mediated mainly by sugars and hormones. ASIL1, a member of the plant-specific trihelix family of DNA-binding transcription factors, was isolated based on its interaction with the GT-element of the Arabidopsis thaliana 2S albumin At2S3 promoter. Mutation of ASIL1 derepressed expression of a subset of embryonic genes resulting in accumulation of 2S albumin and embryo-specific lipids in leaves. 1 It was recently reported that mutation of ASIL1 led to early embryo development in Arabidopsis. 2 In this study, we demonstrated that ASIL1 acts as a temporal regulator of seed filling. In developing siliques, mutation of ASIL1 led to earlier expression of master regulatory genes LEC2, FUS3 and ABI3 as well as genes for seed storage reserves. Moreover, the 12S globulin accumulated to a much higher level in the developing seeds of asil1-1 compared to wild type. To our knowledge, this is the first evidence that ASIL1 not only functions as a negative regulator of embryonic traits in seedlings but also contributes to the maintenance of precise temporal control of seed filling. Thus, ASIL1 represents a novel component of the regulatory framework controlling embryonic gene expression in Arabidopsis.

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Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

Section: Asil1 Is Required For Proper Timing Of Seed Filling In Arabimentioning

confidence: 99%

Section: Asil1 Is Required For Proper Timing Of Seed Filling In Arabimentioning

confidence: 99%

Section: Asil1 Is Required For Proper Timing Of Seed Filling In Arabimentioning

confidence: 99%

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ASIL1 is required for proper timing of seed filling in Arabidopsis

Gao

Lui

et al. 2011

Plant Signaling & Behavior

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REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins

et al. 2018

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The nucleoplasm and nucleolus are the two main territories of the nucleus. While specific functions are associated with each of these territories (such as mRNA synthesis in the nucleoplasm and ribosomal rRNA synthesis in the nucleolus), some proteins are known to be located in both. Here, we investigated the molecular function of REBELOTE ( RBL ), an Arabidopsis thaliana protein previously characterized as a regulator of floral meristem termination. We show that RBL displays a dual localization, in the nucleolus and nucleoplasm. Moreover, we used direct and global approaches to demonstrate that RBL interacts with nucleic acid‐binding proteins. It binds to the NOC proteins SWA 2, At NOC 2 and At NOC 3 in both the nucleolus and nucleoplasm, and also to OBE 1 and VFP 3/ ENAP 1. Taking into account the identities of these RBL interactors, we hypothesize that RBL acts both in ribosomal biogenesis and in the regulation of gene expression.

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A reevaluation of the role of the ASIL trihelix transcription factors as repressors of the seed maturation program

et al. 2021

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Developmental transitions are typically tightly controlled at the transcriptional level. Two of these transitions involve the induction of the embryo maturation program midway through seed development and its repression during the vegetative phase of plant growth. Very little is known about the factors responsible for this regulation during early embryogenesis, and only a couple of transcription factors have been characterized as repressors during the postgerminative phase. Arabidopsis 6b‐INTERACTING PROTEIN‐LIKE1 (ASIL1), a trihelix transcription factor, has been proposed to repress maturation both embryonically and postembryonically. Preliminary data also suggested that its closest paralog, ASIL2, might play a role as well. We used a transcriptomic approach, coupled with phenotypical observations, to test the hypothesis that ASIL1 and ASIL2 redundantly turn off maturation during both phases of growth. Our results indicate that, contrary to what was previously published, neither of the ASIL genes plays a role in the regulation of maturation, at any point during plant development. Analyses of gene ontology (GO)‐enriched terms and published transcriptomic datasets suggest that these genes might be involved in responses during the vegetative phase to certain biotic and abiotic stresses.

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Repression of Seed Maturation Genes by a Trihelix Transcriptional Repressor inArabidopsisSeedlings

Cited by 134 publications

References 81 publications

ASIL1 is required for proper timing of seed filling in Arabidopsis

ASIL1 is required for proper timing of seed filling in Arabidopsis

REBELOTE, a regulator of floral determinacy in Arabidopsis thaliana, interacts with both nucleolar and nucleoplasmic proteins

A reevaluation of the role of the ASIL trihelix transcription factors as repressors of the seed maturation program

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