Repression of the c-Jun trans-Activation Function by the Adenovirus Type 12 E1A 52R Protein Correlates with the Inhibition of Phosphorylation of the c-Jun Activation Domain

Peroxisome Proliferator-activated Receptor α Negatively Regulates the Vascular Inflammatory Gene Response by Negative Cross-talk with Transcription Factors NF-κB and AP-1

“…Although so far most coactivators have been shown to interact with the nuclear receptor LBD, the N-terminal part of the thyroid receptor has also been shown to mediate coactivator interaction (56). Finally, and as already stated above, PPAR␣ is also able to interact with the DNA-binding domain of p65, as well as with the JNK-responsive part of c-Jun, whereas both proteins have already been described to associate with CBP (40,57,58). Hence, the various mutual interactions between the different transcription factors involved and/or CBP as well as their relative abundance may therefore be the critical parameters to determine the actual state of activation and/or repression.…”

E1A12S-mediated Activation of the Adenovirus Type 12 E2 Promoter Depends on the Histone Acetyltransferase Activity of p300/CBP

“…Combined GST Pull-down and HAT Assays-GST fusion proteins were expressed in Escherichia coli BL21 bacteria and purified as described (34). His 6 -E1A 12S was purified from bacterial extracts according to the manufacturer's protocol (Qiagen).…”

A cis-acting element 7 bp upstream of the ESF-1-binding motif is involved in E1A 13S autoregulation of the adenovirus 12 TS2 promoter.

“…For each transient transfection 20 µg of Qiagen-purified plasmid DNA and for co-transfection 10 µg of the pRc\RSV-13S expression vector (Invitrogen) containing the 13S cDNA of Ad12 E1A, a generous gift of D. Brockmann (Brockmann et al, 1995), were used. Cells (1n5i10( cells\ml ; 4i10' cells per experiment) were harvested by centrifugation at 200 g for 10 min at 4 mC and IIB B. M. Pu$ tzer and others B. M. Pu$ tzer and others resuspended in cytomix ( Van den Hoff et al, 1992) ; 225 µl of cells and 25 µl of DNA were mixed and pipetted carefully into the bottom of a new 0n4 cm cuvette (Bio-Rad).…”

“…It codes for two predominant proteins, 266R and 235R, translated from the 13S and 12S mRNA. Both proteins fulfil multiple regulatory functions, including transcriptional activation or inhibition of viral (Berk et al, 1979) and cellular genes (Brockmann et al, 1995 ;De Groot et al, 1991). Moreover, they are essential for the immortalization of primary rodent cells (Lynch & Trainer, 1989) and for complete cell transformation in co-operation cells.…”