Repressor Mutant Forms of the
            <i>Azospirillum brasilense</i>
            NtrC Protein

Huergo, Luciano F.; Assumpção, Marcelo Constantino; Souza, Emanuel M.; Steffens, Maria; Yates, M. G.; Chubatsu, Leda S.; Pedrosa, Fábio O.

doi:10.1128/aem.70.10.6320-6323.2004

Cited by 2 publications

(1 citation statement)

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“…23 The results in the wild-type strain showed that GlnB is poorly expressed under high N, and its accumulation is induced upon N-limitation (Figure S2). These results are in agreement with previous analysis of the regulation of glnB transcription by NtrC, 16,20,33 therefore confirming that the conditions used reflect nitrogen sufficiency and limitation.…”

Section: Western Blot Analysis Of Glnb and Glnz Abundance In A Brasil...supporting

confidence: 92%

Proteomic and Metabolomic Analysis of Azospirillum brasilense ntrC Mutant under High and Low Nitrogen Conditions

et al. 2019

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Azospirillum brasilense is a diazotrophic microorganism capable of associating with roots of important grasses and cereals, promoting plant growth and increasing crop yields. Nitrogen levels and the Ntr regulatory system control the nitrogen metabolism in A. brasilense. This system comprises the nitrogen regulatory proteins GlnD, which is capable of adding uridylyl groups to the PII proteins, GlnB (PII-1) and GlnZ (PII-2), under limiting nitrogen levels. Under such conditions, the histidine kinase NtrB (nitrogen regulatory protein B) cannot interact with GlnB and phosphorylate NtrC (nitrogen regulatory protein C). The phosphorylated form of NtrC acts as a transcriptional activator of genes involved in the metabolism of alternative nitrogen sources. Considering the key role of NtrC in nitrogen metabolism in A. brasilense, in this work we evaluated the proteomic and metabolomic profiles of the wild-type FP2 strain and its mutant ntrC grown under high and low nitrogen. Analysis of the integrated data identifies novel NtrC targets, including proteins involved in the response against oxidative stress (i.e., glutathione S-transferase and hydroperoxide resistance protein), underlining the importance of NtrC to bacterial survival under oxidative stress conditions.

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