Reproducibility, accuracy and concordance of Accutrend® Plus for measuring circulating lipid concentration in adults

Abstract

IntroductionThe determination of lipid biomarkers by capillary sampling may be useful in the screening, diagnosis and/or personal management of hyperlipidemia and cardiovascular risk. It remains unclear whether the use of the Accutrend® Plus system is appropriate. This study aimed to assess its reproducibility, accuracy and concordance for blood lipid profiling in adults.Materials and methods:Fasting capillary total cholesterol (TC) and triglyceride (TG) concentration on Accutrend® Plus were compared with their venous analogues obtained by a laboratory reference method in sixty-one adults (27 men and 34 women, aged 33.0 years). Supplementary capillary sampling was performed at two consecutive days taking into account macro-nutrient intake.Results:The day-to-day reproducibility of the Accutrend® Plus system proved to be high for TC (ICC = 0.85, P < 0.001), but moderate for TG (ICC = 0.68, P < 0.001). Strong correlations (r ≥ 0.80, P < 0.001) with the reference method were found for TC and TG. Mean difference (limits of agreement) were: 0.26 mmol/L (−0.95, 1.47) for TC, and −0.16 mmol/L (−1.29, 0.98) for TG. The concordance for subject classification according to the National Cholesterol Education Program (NCEP) guidelines was significant (P < 0.001), with substantial agreement for TC (κw = 0.67), and moderate agreement for TG (κw = 0.50).Conclusions:Day-to-day reproducibility of the Accutrend® Plus device for TC and TG is not optimal and lacks accuracy when compared to the reference laboratory method. The concordance between both methods for classifying subjects according to the NCEP is inadequate. Accutrend® Plus device should not be interchangeably used as a substitution for the standard laboratory methods in the diagnosis of hyperlipidemia.

Determination of the Relationship between Healthy Lifestyle Behaviors, Physical Fitness, and Risk Factors of Coronary Heart Diseases in University Students

“…The intra assay precision of Accutrend ® Plus, as determined by the manufacturer, was 3.7% and 3.4% for TC and TG respectively. The inter-assay precision for Accutrend ® Plus determined with control solution by the manufacturer was lower than 5.0% for TC and 2.4% for TG [20].…”

Design and challenges of a randomized controlled trial for reducing risk factors of metabolic syndrome in Mexican women through water intake

“…Evidence shows that the concordance to classify subjects according to their triglyceride levels of Accutrend is low compared with the standard laboratory methods. 31 We thus lowered the SSB selection cutoff to > 250 kcal/day in October 2009. Literature suggests that adults consuming > 250 kcal/day of SSBs can realistically reduce consumption anywhere from 100 to 150 kcal/day.…”

Validity of a Portable Glucose, Total Cholesterol, and Triglycerides Multi-Analyzer in Adults

“…In their study, Mendez-González et al (2009) found that the error rate for the Accutrend Plus device exceeded NCEP recommendations for total cholesterol but fell below it for triglycerides. Scafoglieri et al (2012) found that the concordance between methods for at-risk subject classification was not adequate according to the NCEP recommendations. The differences between our findings in the present study and the findings of Mendez-González et al and Scafoglieri et al may be at least partially explained by methodological differences, including sample size, criteria for categorization of blood variables, and statistical analysis procedures.…”