Reproducibility and validity of an FFQ to assess usual intake of methyl-group donors

Pauwels, Sara; Doperé, Ine; Huybrechts, Inge; Godderis, Lode; Koppen, Gudrun; Vansant, Greet

doi:10.1017/s1368980014003140

Cited by 17 publications

(19 citation statements)

References 33 publications

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“…Databases of neighboring countries or the USDA database for choline and betaine 33 content were used in the validation of the food frequency questionnaire (FFQ). 34,35 For folate, the Dutch NEVO food composition database was used 36 and the German BLS Nutrient database for methionine. 37 The USDA database was also used for the nutrient content of folate and methionine if not found in NEVO and BSL databases respectively.…”

Section: Discussionmentioning

confidence: 99%

“…A food-frequency questionnaire (FFQ) was developed and validated to assess maternal intake of dietary methyl-group donors (methionine, folate, betaine, and choline) before and during each trimester of pregnancy. 34,35 Twentyfour FFQs were obtained before pregnancy, 96 FFQs at 11-13 weeks, 89 FFQs at 18-22 weeks, and 83 at 30-34 weeks of pregnancy. To assess the intake of methyl-group donors through supplement use, questions were asked about the use of nutritional supplements (frequency, brand/type, dosage) before and during each trimester of pregnancy.…”

Section: Maternal and Neonatal Measurementsmentioning

confidence: 99%

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Dietary and supplemental maternal methyl-group donor intake and cord blood DNA methylation

et al. 2016

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Maternal nutrition is critically involved in the development and health of the fetus. We evaluated maternal methyl-group donor intake through diet (methionine, betaine, choline, folate) and supplementation (folic acid) before and during pregnancy in relation to global DNA methylation and hydroxymethylation and gene specific (IGF2 DMR, DNMT1, LEP, RXRA) cord blood methylation. A total of 115 mother-infant pairs were enrolled in the MAternal Nutrition and Offspring's Epigenome (MANOE) study. The intake of methylgroup donors was assessed using a food-frequency questionnaire. LC-MS/MS and pyrosequencing were used to measure global and gene specific methylation, respectively. Dietary intake of methyl-groups before and during pregnancy was associated with changes in LEP, DNMT1, and RXRA cord blood methylation. Statistically significant higher cord blood LEP methylation was observed when mothers started folic acid supplementation more than 6 months before conception compared with 3-6 months before conception (34.6 § 6.3% vs. 30.1 § 3.6%, P D 0.011, LEP CpG1) or no folic acid used before conception (16.2 § 4.4% vs. 13.9 § 3%, P D 0.036 for LEP CpG3 and 24.5 § 3.5% vs. 22.2 § 3.5%, P D 0.045 for LEP mean CpG). Taking folic acid supplements during the entire pregnancy resulted in statistically significantly higher cord blood RXRA methylation as compared with stopping supplementation in the second trimester (12.3 § 1.9% vs. 11.1 § 2%, P D 0.008 for RXRA mean CpG). To conclude, long-term folic acid use before and during pregnancy was associated with higher LEP and RXRA cord blood methylation, respectively. To date, pregnant women are advised to take a folic acid supplement of 400 mg/day from 4 weeks before until 12 weeks of pregnancy. Our results suggest significant epigenetic modifications when taking a folic acid supplement beyond the current advice.

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Section: Discussionmentioning

confidence: 99%

Section: Maternal and Neonatal Measurementsmentioning

confidence: 99%

Dietary and supplemental maternal methyl-group donor intake and cord blood DNA methylation

et al. 2016

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“…A food frequency questionnaire (FFQ) was used to assess the intake of folate and choline, with 74 women completing the FFQ. 24 The detailed procedure has been described elsewhere. 25 Shortly, portion size was multiplied by the frequency of consumption and the amount of folate in 100 g of consumed products.…”

Section: Methodsmentioning

confidence: 99%

Associations between folate and choline intake, homocysteine metabolism, and genetic polymorphism of MTHFR, BHMT and PEMT in healthy pregnant Polish women

Chmurzyńska

Seremak‐Mrozikiewicz

Malinowska

et al. 2019

Nutrition & Dietetics

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Aim: Physiological homocysteine (Hcy) concentrations depend on several factors, both dietary (including folate and choline intake) and biological (such as polymorphism of the genes involved in Hcy metabolism). This study aimed to thus test the associations between genes functionally linked with Hcy metabolism (MTHFR, BHMT and PEMT), folate and choline intakes, and total Hcy (tHcy) concentrations of healthy pregnant women. Methods: One hundred and three healthy Polish women aged 18-44 years, in the third trimester of pregnancy, were enrolled. Results: Mean blood tHcy and glutathione (GSH) concentrations were 8.08 AE 3.25 μM and 4.84 AE 1.21 μM, respectively. Concentrations of tHcy were found to be lower in the women who were taking folic acid supplements than in those who did not take these supplements (7.42 AE 1.78 μM vs 9.28 AE 4.42 μM, P < 0.05). There were no associations found between the examined parameters and BHMT (rs7356530), MTHFR (rs1801133) and PEMT (rs12325817) alone. However, blood tHcy concentrations differed in the PEMT genotype subgroups when choline and folate intakes were considered: respectively, 25% and 20% lower levels were observed in the C allele carriers who met their needs of choline or folate than in those who did not take enough these nutrients (P < 0.05 for both associations).Conclusions: This study suggests that choline and folate intakes might interact with MTHFR, BHMT and PEMT polymorphisms to determine tHcy and GSH blood concentrations in healthy pregnant women.

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“…Another limitation is the fact that the Belgian food composition database NUBEL [22] does not contain information about the four methyl-group donors under study. Databases of neighboring countries or the USDA database for choline and betaine [25] content were used in the validation of the FFQ [20,21]. For folate, the Dutch NEVO food composition database was used [23] and the German BLS Nutrient database for methionine [24].…”

Section: Discussionmentioning

confidence: 99%

“…To assess maternal intake of dietary methyl-group donors (methionine, folate, betaine, and choline) before and during each trimester of pregnancy of women enrolled in the MANOE study a food-frequency questionnaire (FFQ) was developed and validated. We validated the FFQ in two validation studies (one using a food diary as reference method and one with additional biomarkers of the one-carbon metabolism) in women of reproductive age [20,21]. Ideally, food consumption data are linked to food composition data collected in the same region/country.…”

Section: Methodsmentioning

confidence: 99%

Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy

et al. 2016

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It is still unclear to which extent methyl-group intake during pregnancy can affect maternal global DNA (hydroxyl)methylation. Pregnancy methylation profiling and its link with methyl-group intake in a healthy population could enhance our understanding of the development of pregnancy related disorders. One hundred forty-eight women were enrolled in the MANOE (MAternal Nutrition and Offspring’s Epigenome) study. Thiry-four women were enrolled before pregnancy and 116 during the first trimester of pregnancy. Global DNA (hydroxy)methylation in blood using LC-MS/MS and dietary methyl-group intake (methionine, folate, betaine, and choline) using a food-frequency questionnaire were estimated pre-pregnancy, during each trimester, and at delivery. Global DNA (hydroxy)methylation levels were highest pre-pregnancy and at weeks 18–22 of pregnancy. We observed a positive relation between folic acid and global DNA methylation (p = 0.04) and hydroxymethylation (p = 0.04). A high intake of methionine pre-pregnancy and in the first trimester showed lower (hydroxy)methylation percentage in weeks 11–13 and weeks 18–22, respectively. Choline and betaine intake in the first weeks was negatively associated with hydroxymethylation. Women with a high intake of these three methyl groups in the second and third trimester showed higher hyrdoxymethylation/methylation levels in the third trimester. To conclude, a time trend in DNA (hydroxy)methylation was found and women with higher methyl-group intake showed higher methylation in the third trimester, and not in earlier phases of pregnancy.

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Reproducibility and validity of an FFQ to assess usual intake of methyl-group donors

Cited by 17 publications

References 33 publications

Dietary and supplemental maternal methyl-group donor intake and cord blood DNA methylation

Dietary and supplemental maternal methyl-group donor intake and cord blood DNA methylation

Associations between folate and choline intake, homocysteine metabolism, and genetic polymorphism of MTHFR, BHMT and PEMT in healthy pregnant Polish women

Maternal Methyl-Group Donor Intake and Global DNA (Hydroxy)Methylation before and during Pregnancy

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