Reproducibility of flow mediated skin fluorescence to assess microvascular function

Hellmann, Marcin; Tarnawska, Maria; Dudziak, Maria; Dorniak, Karolina; Roustit, Matthieu; Cracowski, Jean‐Luc

doi:10.1016/j.mvr.2017.05.004

Cited by 25 publications

(18 citation statements)

References 15 publications

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“…As previously demonstrated, the inter-day reproducibility of the FMSF device is excellent [10]. In this study, an assessment was made of inter-observer agreement using the same device in the same subject under identical conditions.…”

Section: Discussionmentioning

confidence: 79%

“…Moreover, it was demonstrated that both ischemic and hyperemic response differentiated between healthy subjects and patients with CAD suggesting microvascular dysfunction in those patients [10]. Therefore, given its relatively low cost and ease of use, FMSF seems a promising tool for the assessment of microvascular endothelial function in CVDs.…”

Section: Introductionmentioning

confidence: 99%

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A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease

et al. 2018

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease

et al. 2018

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“…Since maximum light penetration of 340 nm UVB in the skin is about 0.3 to 0.5 mm, results are determined by fluorescence of NADH within the epidermis [16]. e wavelength of emitted NADH fluorescence (FL) is 460 nm (blue light) [13].…”

Section: Methodsmentioning

confidence: 99%

“…One technique, named flow-mediated skin fluorescence (FMSF), is based on noninvasive, real-time, in vivo measurement of NADH fluorescence, emitted from the skin cells of a forearm in response to the blockage and release of blood flow [12]. A pilot study conducted in patients with coronary artery disease (CAD) demonstrated that FMSF offered feasible and reproducible measurement of NADH which was sensitive to acute changes in the skin blood flow and yielded results associated with established plasma endothelial markers [13].…”

Section: Introductionmentioning

confidence: 99%

Changes of NADH Fluorescence from the Skin of Patients with Systemic Lupus Erythematosus

Bogaczewicz

Tokarska

Woźniacka

2019

BioMed Research International

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Introduction. The blood circulation of the skin is an accessible and representative vascular bed for examining the mechanisms of microcirculatory function. Endothelial function is impaired in systemic lupus erythematosus (SLE), which implies disorders in cell metabolism dependent on blood circulation; however, noninvasive monitoring of metabolism at the tissue and cell level is absent in daily clinical practice. Objective. The aim of the study was to examine changes of NADH fluorescence from the epidermis of a forearm measured with the flow mediated skin fluorescence (FMSF) technique in patients with SLE and to investigate whether they are associated with clinical manifestation of the disease. Materials and Methods. The study enrolled 36 patients with SLE and 34 healthy individuals. Changes of NADH fluorescence were measured using FMSF on the forearm in response to blocking and releasing of blood flow. The results were represented as ischemic (IR max and IR auc) and hyperemic response maximum and area under the curve (HR max and HR auc). Results. IR max, IR auc, HR max, and HR auc were all lower in patients with SLE (p<0.05) compared with controls. All four parameters were negatively correlated (p<0.05) with patient age. No difference was found in NADH fluorescence between SLE patients with malar rash, discoid rash, photosensitivity, oral ulcers, nonerosive arthritis, renal disorder, hematologic disorder, or immunologic disorder and those without. No correlation was revealed between the SLEDAI score and NADH fluorescence. Conclusion. Changes of NADH fluorescence indicate the reduction in NADH restoration, observed especially during reperfusion, and suggest the occurrence of disorders in the microcirculation of the skin and/or at the mitochondrial level. Such changes of NADH during reperfusion in patients with SLE could be associated with their possible lower sensitivity to hypoxia and possibly with endothelial dysfunction.

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“…However, some authors [36] suggest that the decrease in NADH fluorescence intensity during reperfusion not only shows the change in mitochondrial function, but also in microcirculatory and endothelial functions related to the efficiency of blood supply to the skin. Both the skin blood vessels' thermoregulatory [37][38][39] and endothelial [40] functions improved after training.…”

Section: Exercise Responsementioning

confidence: 99%

The Effect of a 7-Week Training Period on Changes in Skin NADH Fluorescence in Highly Trained Athletes

Bugaj¹,

Kusy²,

Kantanista

et al. 2020

Applied Sciences

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The study aimed to evaluate the changes of nicotinamide adenine dinucleotide (NADH) fluorescence in the reduced form in the superficial skin layer, resulting from a 7-week training period in highly trained competitive athletes (n = 41). The newly, non-invasive flow mediated skin fluorescence (FMSF) method was implemented to indirectly evaluate the mitochondrial activity by NADH fluorescence. The FMSF measurements were taken before and after an exercise treadmill test until exhaustion. We found that athletes showed higher post-training values in basal NADH fluorescence (pre-exercise: 41% increase; post-exercise: 49% increase). Maximum NADH fluorescence was also higher after training both pre- (42% increase) and post-exercise (47% increase). Similar changes have been revealed before and after exercise for minimal NADH fluorescence (before exercise: 39% increase; after exercise: 47% increase). In conclusion, physical training results in an increase in the skin NADH fluorescence levels at rest and after exercise in athletes.

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Reproducibility of flow mediated skin fluorescence to assess microvascular function

Cited by 25 publications

References 15 publications

A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease

A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease

Changes of NADH Fluorescence from the Skin of Patients with Systemic Lupus Erythematosus

The Effect of a 7-Week Training Period on Changes in Skin NADH Fluorescence in Highly Trained Athletes

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