Reproducibility of Vessel Density Measurement With Optical Coherence Tomography Angiography in Eyes With and Without Retinopathy

Abstract

Purpose To determine the intra-visit and inter-visit reproducibility of optical coherence tomography angiography (OCTA) measurements of macular vessel density in eyes with and without retinal diseases. Methods Fifteen healthy volunteers and 22 patients with retinal diseases underwent repeated OCTA (Angiovue Imaging System, Optovue Inc.) scans after pupil dilation on two separate visit days. For each visit day, the eyes were scanned twice. Vessel density defined as the proportion of vessel area with flowing blood over the total measurement area was calculated using Angiovue software. Intra-visit and inter-visit reproducibility were summarized as coefficients of variation (CV) and intra-class correlation coefficients (ICC) calculated from variance component models. Results The CVs representing the intra-visit reproducibility of the superficial macular vessel density measurements for different quadrants on 3 × 3 mm scans varied from 2.1–4.9% and 3.4–6.8% for healthy and diseased eyes respectively, and for the inter-visit it was 2.9–5.1% and 4.0–6.8% respectively. The CVs were lower in healthy eyes than in diseased eyes, lower for intra-visit than for inter-visit, lower on 3 × 3 mm scans than on 6 × 6 mm scans, and lower for paracentral subfields than for central subfield. Conclusion The evidence presented here demonstrates good reproducibility of OCTA for measurement of superficial macula vessel density in both healthy eyes and eyes with diabetic retinopathy without diabetic macular edema.

Macular Vessel Density Measured With Optical Coherence Tomography Angiography and Its Associations in a Large Population-Based Study

“…Mean macular vessel density of the 1631 participants with a mean age of 50 (range, 18-92) years was 47.3% and 55.1% for the superficial and deep retinal layers, respectively. You et al 6 reported a mean global macular superficial vessel density of 53.8% in 15 healthy subjects with a mean age of 49 (range, 37-54) years, when measured and analyzed with the AngioVue system. In the study by Wang et al, 11 105 healthy participants with a mean age of 36 (range, 17-64) years were scanned with the AngioVue system.…”

“…[3][4][5] Previous studies have demonstrated high intravisit repeatability and intervisit reproducibility for OCTA measurements of parapapillary and macular perfusion. [6][7][8][9][10] Using OCTA, macular vessel density in healthy volunteers recently was quantified and its associations with age, sex, and refractive errors were assessed. [11][12][13][14] Studies also showed the clinical applicability of OCTA for diagnosis and management of glaucoma, and retinal and choroidal diseases.…”

Macular vascular circulation and retinal oxygen saturation changes for idiopathic macular epiretinal membrane after vitrectomy

“…; You et al. ). The blood flow fluctuations are converted to decorrelation signals recognizable and identifiable from the static region, thus quantifying the measurement of the retinal and choroidal blood flow at the en face view (Jia et al.…”

Macular Perfusion Parameters in Different Angiocube Sizes: Does The Size Matter in Quantitative Optical Coherence Tomography Angiography?

“…Many studies have reported that these parameters have high intraand interoperator reproducibility both in eyes with and without retinal diseases, for the same scan type and for the same machine. [7][8][9][10][11][12][13] In a study by Dong and coworkers, 14 a good agreement between 3 3 3-and 6 3 6-mm angiocubes was found for choriocapillary vessel density and for FAZ area at all retina vasculatures; conversely, reliability for retinal vessel density was weak. However, scan interchangeability has been only tested for small angiocubes and it remains uncertain for wider size scans (e.g., 12 3 12 mm).…”