Reproduction and Sex Control in Turbot

Taboada, Xoana; Robledo, Diego; Bouza, Carmen; Piferrer, Francesc; Viñas, Ana; Martı́nez, Paulino

doi:10.1002/9781119127291.ch28

Cited by 5 publications

(5 citation statements)

References 73 publications

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“…Our data strongly suggest a complex genetic architecture of turbot SD, supporting previous information Taboada et al, 2019). Despite the major SD region drives GD in nearly 80% of the fish, and close to 90% of the families show a significant association between sex and markers in that region, other genetic and environmental factors interact with the main locus to fate the sex of each fish.…”

Section: Turbot Sd As a Complex Traitsupporting

confidence: 89%

“…confirmed that SD of turbot mainly relies on a small region at the proximal end of LG5 and follows a ZW/ZZ pattern of inheritance (Haffray et al, 2009;Martínez et al, 2009;Taboada et al, 2019). However, unlike previous reports, here we gathered enough evidences to suggest sox2 as the major SDg in this species: i) it is located in the narrow window established after segregation analysis and surrounded by the two most significant SNPs identified by GWAS; and ii) it is the only gene among the transcripts expressed along GD in the delimited SD region that showed a differential expression between males and females within a short five days window (50-55 dpf), just before the first signals of germ cell proliferation (up-regulation of gsdf1 and vasa at 60-65 dpf; Robledo et al, 2015).…”

Section: The Main Sex Determining Gene (Sdg) Of Turbotmentioning

confidence: 59%

“…For this study, we went beyond the important information previously gathered on sex determination (SD) and gonad differentiation (GD) in turbot by combining genomics and classical genetics approaches, as well as using functional and statistical association methodologies. A large number of families and individuals, as well as a careful selection of biological material was used to ensure the genetic constitution of fishes considering the complex architecture of SD reported in turbot (Taboada et al, 2019). We got a comprehensive picture of the complex mechanism underlying SD in this species and framed this information within teleost SD evolution.…”

Section: Discussionmentioning

confidence: 99%

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Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)

Martı́nez

Robledo

Taboada

et al. 2019

Preprint

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A major challenge in evolutionary biology is to find an explanation for the variation in sex-determining (SD) systems across taxa and to understand the mechanisms driving sex chromosome differentiation. We studied the turbot, holding a ZW/ZZ SD system and no sex chromosome heteromorphism, by combining classical genetics and genomics approaches to disentangle the genetic architecture of this trait. RAD-Seq was used to genotype 18,214 SNPs on 1,135 fish from 36 families and a genome wide association study (GWAS) identified a ~ 6 Mb region on LG5 associated with sex (P < 0.05). The most significant associated markers were located close to sox2, dnajc19 and fxr1 genes. A segregation analysis enabled narrowing down the associated region and evidenced recombination suppression in a region overlapping the candidate genes. A Nanopore/Illumina assembly of the SD region using ZZ and WW individuals identified a single SNP fully associated with Z and W chromosomes. RNA-seq from 5-90 day-old fish detected the expression along the gonad differentiation period of a short non-coding splicing variant (ncRNA) included in a vertebrate-conserved long non-coding RNA overlapping sox2. qPCR showed that sox2 was the only differentially expressed gene between males and females at 50-55 days post fertilization, just prior the beginning of gonad differentiation. More refined information on the involvement of secondary genetic and environmental factors and their interactions on SD was gathered after the analysis of a broad sample of families. Our results confirm the complex nature of SD in turbot and support sox2 as its main driver.

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Section: Turbot Sd As a Complex Traitsupporting

confidence: 89%

Section: The Main Sex Determining Gene (Sdg) Of Turbotmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)

Martı́nez

Robledo

Taboada

et al. 2019

Preprint

Self Cite

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“…We provide a co-dominant marker-based method that can also be used for other research in sturgeons, such as gynogenesis. Gynogenesis plays a crucial role in the study of sex determination in fish and in the production of sex-controlled individuals, such as in turbot [18]. As gynogenesis technology artificially induces the multiplication of haploid gametes into polyploid individuals, inactivated sperm from closely related species is often used to fertilize fish eggs.…”

Section: Discussionmentioning

confidence: 99%

InDel DNA Markers Potentially Unique to Kaluga Sturgeon Huso dauricus Based on Whole-Genome Resequencing Data

et al. 2023

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Sturgeons are ancient and endangered species whose populations have been greatly reduced over the past few centuries due to habitat destruction and overfishing for the production of expensive caviar. All sturgeon species are listed in CITES Appendix II to limit their trade; therefore, accurate species identification is crucial. In this study, we performed whole-genome resequencing of five sturgeon species, including Kaluga sturgeon (H. dauricus), Siberian sturgeon (A. baerii), Sterlet sturgeon (A. ruthenus), Russian sturgeon (A. gueldenstaedtii), and Amur sturgeon (A. schrenckii), to obtain species-specific InDel-based nucleotide sequences for Kaluga sturgeon. Through whole-genome screening within these five sturgeon species, we developed methods for the rapid identification of Kaluga sturgeon germplasm. Using dominant/co-dominant molecular markers designed for Huso dauricus, purebred or hybrid samples can be identified through a PCR reaction. These markers allow for the precise identification of Kaluga sturgeon lineages from at least seven sturgeon species (H. dauricus, A. schrenckii, A. ruthenus, A. baerii, A. gueldenstaedtii, A. stellatus, and H. huso) and their hybrids. This development is expected to have a positive effect on both the sturgeon trade and the conservation of sturgeon germplasm resources.

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“…However, turbot cannot naturally spawn eggs in captivity, i.e., they need to be hand-stripped from ripe fish and artificially fertilized [37]. Numerous studies of turbot reproduction have focused on sex control [38], environmental impact (salinity temperature and photoperiod) [39][40][41], exogenous drug stimulation [42,43] and HPG axisrelated genes functions during the reproductive cycle [37,[44][45][46][47]. These studies have laid a solid foundation for further exploring the reproductive physiology and endocrinology of turbot.…”

Section: Introductionmentioning

confidence: 99%

Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro

Zhang,

Li,

Zhang

et al. 2023

Antioxidants

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High-quality eggs are essential for the sustainability of commercial aquaculture production. Melatonin is a potent candidate for regulating the growth and maturation of oocytes. Therefore, research on the effect of melatonin on marine fish oocytes in vitro has been conducted. The present study successfully established a culture system of turbot (Scophthalmus maximus) oocytes in vitro and investigated the effect of melatonin on oocyte meiotic maturation, antioxidant capacity, and the expression of apoptosis-related genes. The cultures showed that turbot Scophthalmus maximus late-vitellogenic denuded oocytes, with diameters of 0.5–0.7 mm, had a low spontaneous maturation rate and exhibited a sensitive response to 17α, 20β-dihydroxyprogesterone (DHP) treatment in vitro. Melatonin increased by four times the rate of oocyte germinal vesicle breakdown (GVBD) in a concentration- and time-dependent manner. The mRNA of melatonin receptor 1 (mtnr1) was significantly upregulated in the oocyte and follicle after treatment with melatonin (4.3 × 10−9 M) for 24 h in vitro, whereas melatonin receptor 2 (mtnr2) and melatonin receptor 3 (mtnr3) remained unchanged. In addition, melatonin significantly increased the activities of catalase, glutathione peroxidase, and superoxide dismutase, as well as the levels of glutathione, while decreasing the levels of malondialdehyde and reactive oxygen species (ROS) levels in turbot oocytes and follicles cultures in vitro. p53, caspase3, and bax mRNAs were significantly downregulated in oocytes and follicles, whereas bcl2 mRNAs were significantly upregulated. In conclusion, the use of turbot late-vitellogenesis oocytes (0.5–0.7 mm) is suitable for establishing a culture system in vitro. Melatonin promotes oocyte meiotic maturation and antioxidative capacity and inhibits apoptosis via the p53-bax-bcl2 and caspase-dependent pathways, which have important potential to improve the maturation and quality of oocytes.

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Reproduction and Sex Control in Turbot

Cited by 5 publications

References 73 publications

Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)

Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)

InDel DNA Markers Potentially Unique to Kaluga Sturgeon Huso dauricus Based on Whole-Genome Resequencing Data

Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro

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