Reproduction of Adenoviral Gizzard Erosion by the Horizontal Transmission of Fowl Adenovirus Serotype 1

Ono, Masaaki; Okuda, Yo; Shibata, Isao; Sato, Shizuo; Okada, Kōsuke

doi:10.1292/jvms.69.1005

Cited by 33 publications

(19 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fact that reinforces this hypothesis is that within the same farm no FAdV infections were detected in poultry houses with progeny originating from a breeder flock other than the FAdVsuspected breeder flock. This fact additionally confirms that proper biosecurity measures are effective in preventing horizontal transmission of FAdV-1 causing gizzard erosion (Ono et al, 2007). The vertical transmission of FAdV was discussed elsewhere (McConnell Adair and Fitzgerald, 2008).…”

Section: Discussionsupporting

confidence: 68%

Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions

et al. 2011

View full text Add to dashboard Cite

Broiler chickens with clinical signs of uneven growth, depression, and dull feathers were submitted to our laboratory and, at necropsy, lesions in proventriculus, gizzard, and intestines were detected. Fowl adenovirus serotype 1 (FAdV-1) was isolated from digestive tissues. The virus, assigned as FAdV-PL/G068/08, showed 99.5% nucleotide homology and 99.2% amino acid homology in hexon gene with chicken embryo lethal orphan (CELO) strain classified as the European reference of FAdV-1. One-day-old and 21-d-old SPF chickens were inoculated with FAdV-PL/068/08 by both nasal and ocular routes and then observed daily and examined by necropsy at 6, 10, and 14 d postinoculation. Experimental infection with isolated virus was fatal for younger chickens and major lesions occurred in the gizzards. No clinical or pathological changes were observed in chickens infected at 21 d of age, but the presence of intranuclear inclusion bodies in gizzard epithelial cells was detected. Molecular characterization was based on the long and short fibers genes sequencing and comparison of obtained sequences with other FAdV-1 strains. The homology between FAdV-PL/G068/08 and other sequences available in GenBank was between 98.9 and 99.8% (short fiber region) and 99.0 and 99.7% (long fiber region) at nucleotide level and between 98.4 and 100% (short fiber region) and 99.3 and 99.9% (long fiber region) at amino acid level. No correlation between identified amino acid changes in short and long fiber proteins and pathogenicity of studied FAdV-1 strains was observed. Although short and long proteins were indicated as factors influencing virus pathogenicity, the role of identified sequence differences in infectivity determination remain unclear.

show abstract

Section: Discussionsupporting

confidence: 68%

Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Various factors, such as the age of the bird, differences in challenge dose used for the experimental infection, and differences in the route of infection, can make the interpretation of onset and severity of clinical signs and lesions difficult (Ono et al, 2007). In these experiments, the route of infection caused significant differences in the presentation of the lesions.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of gizzard erosion associated with fowl adenovirus infection in Korea

Lim

Kim

et al. 2012

Poultry Science

View full text Add to dashboard Cite

The pathogenicity of a fowl adenovirus serotype-1 (FAdV-1, K181 strain) isolated from a case of gizzard erosion in layer chickens was investigated in specific-pathogen-free (SPF) chicks. One-week-old SPF chicks were inoculated orally or intramuscularly with the isolate of FAdV-1 and euthanized for necropsy at 7, 14, and 21 d postinoculation. Although there were no clinical signs after inoculation, gizzard erosions were observed grossly and the virus was recovered from the gizzards in the inoculated chickens. Histologically, in the chickens that were infected orally, the lesions found in the gizzard consisted of severe degeneration and necrosis of glandular epitheliums and eosinophilic inclusion bodies. These results indicate that the Korean FAdV-1 isolate could induce gizzard lesions in chickens. Moreover, the present investigation reproduced an outbreak of gizzard erosion caused by FAdV-1 infection and, for the first time, described the isolation of FAdV-1 from chickens in Korea. These findings provide important information on the epidemiology and pathogenesis of FAdV-1 infection in chickens.

show abstract

“…In previous studies the use of techniques such as microtiter virus neutralization tests (11,16,33,(38)(39)(40), PCR alone (49,50,59) or in combination with either sequencing (2,14,15,30,32,48) or restriction fragment polymorphism (RFLP) (29,44,54), and a combination of these techniques (7,9,10,20,37,41,42,57) have been used to identify FAdV serotypes. Although each of these techniques is readily available to most laboratories, they have limitations, including the lengthy processes involved, need for extensive interpretation, and potential for indeterminate results, for example, the cross-neutralization of serum samples (7,10,16,27).…”

Section: Discussionmentioning

confidence: 99%

Classification of Fowl Adenovirus Serotypes by Use of High-Resolution Melting-Curve Analysis of the Hexon Gene Region

et al. 2009

View full text Add to dashboard Cite

Identification of fowl adenovirus (FAdV) serotypes is of importance in epidemiological studies of disease outbreaks and the adoption of vaccination strategies. In this study, real-time PCR and subsequent highresolution melting (HRM)-curve analysis of three regions of the hexon gene were developed and assessed for their potential in differentiating 12 FAdV reference serotypes. The results were compared to previously described PCR and restriction enzyme analyses of the hexon gene. Both HRM-curve analysis of a 191-bp region of the hexon gene and restriction enzyme analysis failed to distinguish a number of serotypes used in this study. In addition, PCR of the region spanning nucleotides (nt) 144 to 1040 failed to amplify FAdV-5 in sufficient quantities for further analysis. However, HRM-curve analysis of the region spanning nt 301 to 890 proved a sensitive and specific method of differentiating all 12 serotypes. All melt curves were highly reproducible, and replicates of each serotype were correctly genotyped with a mean confidence value of more than 99% using normalized HRM curves. Sequencing analysis revealed that each profile was related to a unique sequence, with some sequences sharing greater than 94% identity. Melting-curve profiles were found to be related mainly to GC composition and distribution throughout the amplicons, regardless of sequence identity. The results presented in this study show that the closed-tube method of PCR and HRM-curve analysis provides an accurate, rapid, and robust genotyping technique for the identification of FAdV serotypes and can be used as a model for developing genotyping techniques for other pathogens.Fowl adenoviruses (FAdVs), belonging to the Aviadenovirus genus of the family Adenoviridae (19), have been grouped into five species based on their molecular structure and further divided into 12 serotypes, based largely on cross-neutralization assays (18). There are several strains in each serotype. FAdVs are endemic worldwide and known to cause inclusion body hepatitis, quail bronchitis, hydropericardium syndrome (18, 28), gizzard erosion, and pancreatic necrosis (33,34,43,44,56).Diagnosis of FAdV infections can be made from the observation of gross and histopathological changes in the liver and the use of electron microscopy (3, 11) and various serological tests, such as enzyme-linked immunosorbent assay (3,18,45), agar gel immunodiffusion, counterimmunoelectrophoresis, indirect hemagglutination, immunofluorescence (3), and Southern hybridization (10, 12). Identification of the serotype(s) involved is very useful for epidemiological tracing and is of critical importance where vaccination is to be used for the control of the disease (3, 20). Typing of the virus conventionally requires isolation in cell culture, followed by a virus neutralization assay (16); however, the implementation of this method is lengthy and labor intensive, and cross-reactivity between serotypes can sometimes render results inconclusive. Tests using PCR together with DNA sequencing (22, 59) and/or rest...

show abstract

Reproduction of Adenoviral Gizzard Erosion by the Horizontal Transmission of Fowl Adenovirus Serotype 1

Cited by 33 publications

References 16 publications

Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions

Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions

Outbreak of gizzard erosion associated with fowl adenovirus infection in Korea

Classification of Fowl Adenovirus Serotypes by Use of High-Resolution Melting-Curve Analysis of the Hexon Gene Region

Contact Info

Product

Resources

About