Reproduction of Poxviruses

Moss, Bernard

doi:10.1007/978-1-4684-2703-5_5

Cited by 61 publications

(79 citation statements)

References 306 publications

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“…1) was indistinguishable from that of ectromelia virus in infected mouse livers (Leduc & Bernhard, 1962) or that of other poxviruses (Moss, 1974). In contrast, the response of the cells was significantly different to that normally seen in a wide range of poxvirus-infected tissue culture cells, but remarkably similar to that observed in ectromelia virus-infected mouse livers.…”

Section: Discussionmentioning

confidence: 65%

“…At this time, virus antigen was demonstrable by immunofluorescence in over 95 ~ of cells; many cells also showed focal concentrations of antigen corresponding in size and shape to the eosinophilic inclusions. Ultrastructural examination showed that inclusion bodies were the site of virus replication and can therefore be regarded as 'B'-type inclusions (Moss, 1974) (Fig. 1 and 2).…”

Section: Microscopical Observationsmentioning

confidence: 99%

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Ectromelia Virus-induced Changes in Primary Cultures of Mouse Hepatocytes

Lees

Stephen

1985

Journal of General Virology

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SUMMARYMouse hepatocytes were isolated by collagenase perfusion, maintained in nonproliferating monolayer culture and shown to retain liver cell function as judged by gluconeogenesis for 15 to 18 h. Such cells could be infected with and support the replication of a virulent strain of ectromelia virus. Virus antigen and characteristic cytoplasmic 'B'-type poxvirus inclusion bodies were demonstrated by immunofluorescence in virtually all cells. By electron microscopy it was shown that 'B'-type inclusions were the site of virus replication, and that the biogenesis of ectromelia virus and ultrastructural changes in hepatocytes were similar to those observed in infected mouse livers. Early cell rounding effects, a normal characteristic of poxvirus infections in tissue culture cells, were not seen in ectromelia-infected hepatocytes, although late degenerative changes did occur. Pulse-labelling of hepatocyte cultures with [35S]methionine showed that ectromelia virus inhibited the rise in protein synthesis seen in controls and imposed a gradual decline in host protein synthesis to an extent and at a rate significantly different from that in mouse L929 cells. Gluconeogenesis was inhibited by ectromelia virus infection of hepatocytes.

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Section: Discussionmentioning

confidence: 65%

Section: Microscopical Observationsmentioning

confidence: 99%

Ectromelia Virus-induced Changes in Primary Cultures of Mouse Hepatocytes

Lees

Stephen

1985

Journal of General Virology

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“…Poxviruses have a number of enzymes needed for transcription of early genes in infected cells (Moss, 1990). Among these enzymes is NPH I, which is capable of hydrolysing ATP and dATP.…”

Section: Nucleotide Triphosphate Phosphohydrolase I (Nph I) Genementioning

confidence: 99%

Recent advances in the molecular biology of entomopoxviruses

Arif

1995

Journal of General Virology

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“…These include DNA-dependent RNA polymerase, poly(A) polymerase, guanyl-and methyltransferases, topoisomerase, endoribonuclease and phosphohydrolases (Moss, 1974;Dales & Pogo, 1981). These unique properties of vaccinia virus suggest that the regulatory sequences of the viral genome might have evolved in a different way to those of the host cell.…”

Section: (Accepted 21 March 1984)mentioning

confidence: 99%

“…Since vaccinia virus replicates in the cytoplasm of infected ceils and carries within its particles all of the enzymes required for transcription (Moss, 1974;Dales & Pogo, 1981), it was of interest to determine whether the viral sequences now introduced into the nucleus could also be expressed. To that end, total RNA was extracted from transformants, fractionated by oligo(dT)-cellulose chromatography and both poly(A)-and poly(A) + RNA fractions were analysed for the presence of viral sequences by Northern blot hybridization with appropriate DNA restriction fragments that had been purified from recombinant clones.…”

Section: (Accepted 21 March 1984)mentioning

confidence: 99%

Expression of Cloned Vaccinia Virus DNA Sequences Introduced into Animal Cells

Boni

Esteban

Pellicer

1984

Journal of General Virology

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SUMMARYIndividual cloned HindlII fragments of vaccinia virus DNA were introduced into cells by DNA-mediated gene transfer. The presence of individual fragments in the different transformants was confirmed by Southern blot hybridization analysis. Several of the transformants were found to express viral sequences at various levels. The sizes of the transcripts containing vaccinia virus sequences were highly heterogeneous, with no discrete species of RNA. Positive clones contained vaccinia virus sequences in both the poly(A) ÷ and poly(A)-RNA fractions, although the prevalence of these sequences was variable in the two fractions. The S1 nuclease map of the 5' end of the transcripts from transformants containing the HindlII-J fragment revealed a unique 5' end, similar to RNA from virus-infected cells• In contrast, analysis of the 3' end of RNAs from these transformants showed a high degree of heterogeneity, which might explain the heterogeneity found in Northern blot patterns. In this report, it is shown for the first time that in cells transformed with vaccinia virus DNA there is proper initiation for, at least, the viral thymidine kinase gene.

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Reproduction of Poxviruses

Cited by 61 publications

References 306 publications

Ectromelia Virus-induced Changes in Primary Cultures of Mouse Hepatocytes

Ectromelia Virus-induced Changes in Primary Cultures of Mouse Hepatocytes

Recent advances in the molecular biology of entomopoxviruses

Expression of Cloned Vaccinia Virus DNA Sequences Introduced into Animal Cells

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