Reproduction of Sedum drymarioides, an Endangered Rare Species, by Micropropagation.

Kitamura, Yoshihisa; Kubo, Kouji; Rahman, Laiq ur; Ikenaga, Toshihiko

doi:10.5511/plantbiotechnology.19.303

Cited by 7 publications

(16 citation statements)

References 14 publications

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“…This might be due to specific hormonal action and its concentration during the in vitro culture of C. orbiculata. This was similar with some previous studies (Kitamura et al 2002;Solís et al 2013;Zhao et al 2013). Combinations of 10 lM BA and 2.0 lM NAA or 10 lM TDZ and 0.5 lM NAA were found to be more effective for shoot regeneration in this study, compared to other treatments tested.…”

Section: Discussionsupporting

confidence: 94%

“…In this study, shoots rooted in the same treatments, indicating that plantlet production in C. orbiculata is a synchronous phenomenon. Similar findings have been reported for other plant species (Kitamura et al 2002;Kordi et al 2013). Shoot regeneration failed in combinations of BA or TDZ with 2,4-D or picloram, however, combinations of BA and GA 3 promoted shoot regeneration in C. orbiculata are in accordance with the reports on other plant species (Hepaksoy and Aksoy 2006;Zhao et al 2013).…”

Section: Discussionsupporting

confidence: 86%

“…The present study indicated that significant adventitious shoot production in C. orbiculata is dependent on the type and combination of PGRs. Similar phenomena have also been reported for other plant species (Kitamura et al 2002;Kordi et al 2013;Solís et al 2013).…”

Section: Discussionsupporting

confidence: 69%

“…Synergistic effects in adventitious shoot proliferation is dependent on cytokinincytokinin or cytokinin-auxin or cytokinin-gibberellic acid in C. orbiculata in vitro. Similar effects were also observed in several other species of Crassulaceae (Frello et al 2002;Kitamura et al 2002;Solís et al 2013;Zhao et al 2013). In the current study, leaf explants promoted different morphogenic callus to develop shoot buds except certain individual and combined treatments.…”

Section: Discussionsupporting

confidence: 68%

“…The results indicate that the combination of BA and NAA or TDZ and NAA were important for significantly more shoot production and formation of broad leaves and roots in C. orbiculata. The combined effect of BA or TDZ and NAA on adventitious shoot regeneration has been reported in other Crassulaceae species (Kitamura et al 2002;Kordi et al 2013;Solís et al 2013). In this study, shoots rooted in the same treatments, indicating that plantlet production in C. orbiculata is a synchronous phenomenon.…”

Section: Discussionmentioning

confidence: 90%

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In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

Kumari

Baskaran

Staden

2015

Plant Cell Tiss Organ Cult

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An effective in vitro propagation system via organogenesis was developed for Cotyledon orbiculata, a valuable medicinal plant. Plant regeneration and morphogenesis in vitro of C. orbiculata was initiated from leaf explants using various concentrations and combinations of plant growth regulators (PGRs). The highest number of regenerated shoots (20.0 ± 1.64) and shoot length (2.36 ± 0.27 cm) per explant was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 5 lM thidiazuron and 2.0 lM benzyladenine. The regenerated shoots were elongated (6.47 ± 1.02 cm) when transferred to half-strength MS medium. Elongated shoots rooted (39.60 ± 1.27 per shoot) best in a half-strength MS medium containing 10 lM indole-3-butyric acid. Plantlets were successfully acclimatized (100 %) in a vermiculitesoil mixture (1:1 v/v) in the greenhouse. Rooting was also achieved ex vitro during acclimatization in a vermiculitesoil mixture (1:1 v/v) in the greenhouse with good plant growth. Different tissue types from in vitro culture (callus, shoots and plantlets) and leaves from ex vitro plants exhibited antibacterial activity [minimum inhibitory concentration (MIC) from 0.098 to 0.78 mg ml -1 ] against both Gram-positive and Gram-negative bacteria. In vitro and ex vitro developed plant tissues showed antibacterial activity (MIC of 0.195-0.78 mg ml -1 ) specifically against Klebsiella pneumoniae. Despite this, generally the leaf of ex vitro plants gave good activity (MIC of 0.195-0.78 mg ml -1 ) against both Gram-positive and Gram-negative bacteria. The present study indicated that tissue-culture-derived calli, shoots and plantlets, and ex vitro acclimatized leaves influenced therapeutic potential by the PGRs treatment used in the tissue culture system. The in vitro regenerated plants and its tissues of C. orbiculata using this protocol can be utilized as therapeutic agents for traditional medicine and for ex situ conservation.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 90%

See 3 more Smart Citations

In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

Kumari

Baskaran

Staden

2015

Plant Cell Tiss Organ Cult

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In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Background: As a highly valued medicinal plant, Salvia plebeia R. Brown belongs to the Lamiaceae family that has been subjected to over exploitation in its natural habitat for phytochemical and pharmacological studies. Alternative collection methods need to be developed for the large-scale propagation of Salvia plebeian.Results: Here, e cient and simple, direct organogenesis (from shoot tips and cotyledonary nodes explants) and Globular bodies (GBs) induction (from hypocotyl explants) systems were developed for the in vitro propagation of Salvia plebeia. The highest and number of regenerated shoots (7.0±0.82) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L -1 indole-3-acetic acid (IAA) and 1.0 mg L -1 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L -1 IAA and 1.0 mg L -1 thidiazuron (TDZ) yielded the maximum number of shoots (37.5±1.34) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GBs induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L -1 ) and IAA (0.1 mg L -1 ), and produced GBs that were morphologically similar to globular embryos and successfully germinated on hormone-free MS medium. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate.Conclusions: Taken together, this protocol can be e ciently used for mass propagation, germplasm preservation and likely also for gene transfer of Salvia plebeia.

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In Vitro Propagation via Organogenesis and Formation of Globular Bodies of Salvia Plebeia: A Valuable Medicinal Plant

Wu¹,

Yang²,

Sun³

et al. 2020

Preprint

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Background: As a highly valued medicinal plant, Salvia plebeia R. Brown belongs to the Lamiaceae family that has been subjected to over exploitation in its natural habitat for phytochemical and pharmacological studies. Alternative collection methods need to be developed for the large-scale propagation of Salvia plebeian. Results: Here, efficient and simple, direct organogenesis (from shoot tips and cotyledonary nodes explants) and Globular bodies (GBs) induction (from hypocotyl explants) systems were developed for the in vitro propagation of Salvia plebeia. The highest and number of regenerated shoots (7.0±0.82) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L-1 indole-3-acetic acid (IAA) and 1.0 mg L-1 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L-1 IAA and 1.0 mg L-1 thidiazuron (TDZ) yielded the maximum number of shoots (37.5±1.34) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GBs induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L-1) and IAA (0.1 mg L-1), and produced GBs that were morphologically similar to globular embryos and successfully germinated on hormone-free MS medium. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate. Conclusions: Taken together, this protocol can be efficiently used for mass propagation, germplasm preservation and likely also for gene transfer of Salvia plebeia.

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Reproduction of Sedum drymarioides, an Endangered Rare Species, by Micropropagation.

Cited by 7 publications

References 14 publications

In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

In Vitro Propagation via Organogenesis and Formation of Globular Bodies of Salvia Plebeia: A Valuable Medicinal Plant

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