The estrus cycle is the initial stage of increasing the milk production of PE goats. Estrus detection in PE goats is generally done manually by looking for signs of sound, restlessness, and aggression. The use of voice recording applications to detect estrus has been carried out, one of which is ASM. However, to increase the accuracy of detection based on ASM audio-visual, it is necessary to confirm the cytological status of bovine vaginal cells. This study aimed to detect estrus using audio-visual and vaginal cytology of goats. Eight female PE goats were observed on small breeders for their reproductive cycle, behavior, vocalization with ASM, and cell cytology sampling through vaginal mucus. They are processing data using Spearman rank correlation (SD). The results show aggressive behavior, frequent voices, and signs of clear discharge, and the tail is often moved. ASM detected the sound of goats in estrus at 430.0 Hz – 494.3 Hz and confirmed the superficial cell status, which predominated in PE goats. It can be concluded that the audio of goats in the 472 Hz range recorded using the ASM application can be used as a reference that goats are in estrus and ready to be mated.