Reproductive behaviors promote ecological and phenotypic sexual differentiation in the critically endangered Lehmann’s poison frog

Abstract

Territoriality and parental care are complex reproductive behaviors found in many taxa from insects to mammals. Parental care can be carried out by the female, the male, or both, depending on the species. Territoriality, in contrast, is predominantly displayed by males. Different selective pressures imposed on individuals from the sex performing territorial or parental care behaviors may also lead to sexual differentiation in other life-history traits. Due to their territorial behavior and their diversity of parental care behaviors, Neotropical poison frogs are an excellent study system to investigate whether behavioral traits can influence sexual differentiation in intrinsic or extrinsic traits of individuals. Here, we evaluate whether territorial and parental care behaviors mediate sexual differentiation in ecological (habitat use) and phenotypic (coloration, morphology) traits in the critically endangered Lehmann’s poison frog (Oophaga lehmanni), a species in which males defend territories while females provide parental care. We found sex differences in habitat use and morphological traits, but not in coloration. Males use trunks and green leaves as perches more frequently and are found on higher substrates, than females. We found no sex differences in body size, but females have longer arms than males, which is probably associated with their parental duties (climbing trees to feed the tadpoles). Altogether, our results provide evidence that selection pressures act differently on male and female traits, and that territoriality and parental care may promote the evolution of sexual differentiation in dendrobatids. Long-term wildlife observations are essential to identify important life-history traits and to evaluate hypotheses about the behavioral ecology and conservation of this and other vertebrate species.