Reproductive behaviour, cutaneous morphology, and skin secretion analysis in the anuran Dermatonotus muelleri

Antoniazzi, Marta Maria; Mailho‐Fontana, Pedro Luiz; Nomura, Fausto; Azevedo, Heloisa Bastianon; Pimenta, Daniel Carvalho; Sciani, Juliana Mozer; Carvalho, Fernando Rogério; Rossa-Feres, Denise C.; Jared, Carlos

doi:10.1016/j.isci.2022.104073

Cited by 4 publications

(4 citation statements)

References 54 publications

(100 reference statements)

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“…The presence and function of these glands is influenced by the varying environmental pressures faced by species as well as evolutionary mechanisms which serve to aid species survival and reproduction. For example, frogs in the genus Dermatonutus produce specialized intraspecific chemicals that are excreted from breeding glands, similar to pheromones produced by the mental gland of plethodontid salamanders during the breeding season [ 86 , 87 ]. Likewise, salamander skin has evolved crucial evolutionary mechanisms for purposes such as predator defense and mate attraction; Plethodon shermani has specialized granular glands on the dorsal side of their tail that excrete a toxic, sticky protein that deters predators, while the glands on the ventral side of the tail produce pheromones used for scent marking and courtship-related behaviors [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

“…The biochemical phenotypes inherent to each species, that present as different spectral profiles, are likely because amphibian skin is a complex mucosal organ that performs various critical physiological functions, including respiration, water uptake, ion transport, and innate immunity, whose composition will differ between species [86][87][88]. Amphibian skin serves as a crucial immune organ constituting a sophisticated network of microbiological, physical, immunological, and chemical barriers to pathogen insult, as a result of having a wide range of secretory glands such as mucosal, granular, and adhesive glands [87]. The presence and function of these glands is influenced by the varying environmental pressures faced by species as well as evolutionary mechanisms which serve to aid species survival and reproduction.…”

Section: Plos Computational Biologymentioning

confidence: 99%

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Enhancing predictive performance for spectroscopic studies in wildlife science through a multi-model approach: A case study for species classification of live amphibians

Chen,

Caprio,

Chen

et al. 2024

PLoS Comput Biol

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Near infrared spectroscopy coupled with predictive modeling is a growing field of study for addressing questions in wildlife science aimed at improving management strategies and conservation outcomes for managed and threatened fauna. To date, the majority of spectroscopic studies in wildlife and fisheries applied chemometrics and predictive modeling with a single-algorithm approach. By contrast, multi-model approaches are used routinely for analyzing spectroscopic datasets across many major industries (e.g., medicine, agriculture) to maximize predictive outcomes for real-world applications. In this study, we conducted a benchmark modeling exercise to compare the performance of several machine learning algorithms in a multi-class problem utilizing a multivariate spectroscopic dataset obtained from live animals. Spectra obtained from live individuals representing eleven amphibian species were classified according to taxonomic designation. Seven modeling techniques were applied to generate prediction models, which varied significantly (p < 0.05) with regard to mean classification accuracy (e.g., support vector machine: 95.8 ± 0.8% vs. K-nearest neighbors: 89.3 ± 1.0%). Through the use of a multi-algorithm approach, candidate algorithms can be identified and applied to more effectively model complex spectroscopic data collected for wildlife sciences. Other key considerations in the predictive modeling workflow that serve to optimize spectroscopic model performance (e.g., variable selection and cross-validation procedures) are also discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Computational Biologymentioning

confidence: 99%

Enhancing predictive performance for spectroscopic studies in wildlife science through a multi-model approach: A case study for species classification of live amphibians

Chen,

Caprio,

Chen

et al. 2024

PLoS Comput Biol

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“…The adult skin of P. ephippifer exhibits an organization similar to the skin of other anurans (Felsemburgh et al 2007;Antoniazzi et al 2022). However, it has peculiar characteristics that it shares with some of its congeners.…”

Section: ; Cayuela Et Al 2020)mentioning

confidence: 93%

Larvae to adult: skin ontogeny of Physalaemus ephippifer (Anura: Leptodactylidae)

Santos,

Oliveira-Bahia,

Souza-Ferreira

et al. 2023

Zoomorphology

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Throughout metamorphic development until adulthood, amphibians have important integumentary adaptations that maintain their physiological needs and protection against predation. The evolution of these strategies in the subfamily Leiuperinae has been elucidated in recent years. Therefore, the knowledge about Physalaemus ephippifer's skin attributes can corroborate the changes of these characteristics in the clade. The aim of this study was to assess the characteristics of P. ephippifer regarding the morphological development the skin during their lifespan. We collected foam nests in temporary ponds as well as adult individuals in a forest fragment. In the laboratory, the animals were classi ed by stage development, euthanized, and xed. Sections of the dorsolateral, lumbar, and femoral portions were dissected from the adult individuals. Samples were submitted to histological processing for light microscopy and scanning electron microscopy. In premetamorphosis phase, gland development is absent and secretory cells are present. In prometamorphosis, development of rst glandular rudiments without secretion production begins, also xanthophores. During metamorphosis, mucous glands differentiated before serous glands. Until late metamorphosis ciliated cells persist and epidermis changes to an adultlike. In adults, serous glands show polymorphism with different distributions in the body, despite not having macroglandular structures. In addition, P. ephippifer individuals have few epidermal projections and cryptic coloration. Our results show that despite sharing few morphological structures with other specimens of Leiuperinae subfamily, the characteristics present in individuals of P. ephippifer are appropriate to the niche occupied and consistent with the changes that occur throughout their lineage.

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“…Based on their amphibious habits, the skin microbiota of amphibians can arise from the soil or water, or possibly from the gut microbiota (feces diffuse in the Diversity 2023, 15, 932 2 of 19 environment, which can spread in soil or water to settle on the skin) [9]. The special skin physiology of amphibians keeps them moist, as their skin contains a high number of glands that can secrete mucus [10]. The mucus secreted by their skin glands contains multiple defense molecules (e.g., AMP, alkaloids, lysozyme, and antibodies) [11].…”

Section: Introductionmentioning

confidence: 99%

Ecological Factors and Anthropogenic Disturbance May Restructure the Skin Microbiota of Maoershan Hynobiids (Hynobius maoershanensis)

et al. 2023

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Studies on the skin microbiota of amphibians in different disturbed habitats can clarify the relationship between the skin microbiota composition and environmental factors and have practical implications for the conservation of endangered species. In this study, 16S rRNA high-throughput sequencing was used to profile the skin microbiota of Maoershan hynobiids (Hynobius maoershanensis). Our results illustrate that the alpha diversity of the skin microbiota significantly differed among individuals in higher anthropogenic disturbance-degree (HADD) habitats and lower anthropogenic disturbance-degree (LADD) habitats. The diversity of the skin microbiota in forelimb bud-stage tadpoles from HADD habitats was higher than that in their counterparts from LADD habitats. The richness of the skin microbiota in hindlimb bud-stage tadpoles was greater in HADD habitats than in LADD habitats. However, the alpha diversity of the adult skin microbiota did not differ significantly between the two habitats. Furthermore, stepwise regression analysis indicated that the skin microbiota diversity and relative abundance of dominant bacteria decreased with increasing air temperature, water temperature, and pH; conversely, skin microbiota richness increased with increasing humidity. In addition, the relative abundance of dominant bacteria was influenced by anthropogenic disturbance. We conclude that the skin microbiota of Maoershan hynobiids is affected by ecological factors and anthropogenic disturbance, highlighting the importance of the skin microbiota in response to habitat alteration.

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Reproductive behaviour, cutaneous morphology, and skin secretion analysis in the anuran Dermatonotus muelleri

Cited by 4 publications

References 54 publications

Enhancing predictive performance for spectroscopic studies in wildlife science through a multi-model approach: A case study for species classification of live amphibians

Enhancing predictive performance for spectroscopic studies in wildlife science through a multi-model approach: A case study for species classification of live amphibians

Larvae to adult: skin ontogeny of Physalaemus ephippifer (Anura: Leptodactylidae)

Ecological Factors and Anthropogenic Disturbance May Restructure the Skin Microbiota of Maoershan Hynobiids (Hynobius maoershanensis)

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