Reproductive defects in the abscission mutant <i>ida-2</i> are caused by T-DNA–induced genomic rearrangements

Alling, Renate M.; Galindo-Trigo, Sergio

doi:10.1093/plphys/kiad449

Cited by 5 publications

(10 citation statements)

References 33 publications

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“…All Arabidopsis lines were in the Columbia (Col-0) genetic background, except for the ida-1 mutant (C24; (Butenko et al, 2003)). The previously published mutant and transgenic lines used in this study were: ida-2 (Cho et al, 2008), idaCR (Alling and Galindo-Trigo, 2023), idl1CR (Shi et al, 2018), p35S::IDA (Stenvik et al, 2006), hae hsl2 (Stenvik et al, 2008), wrky57 (Jiang et al, 2012), wrky60-1 (Xu et al, 2006) and wrky48 (Jiang et al, 2012). Genotyping primers are listed in the Supplemental Table S1.…”

Section: Plant Material Growth Conditions and Treatmentsmentioning

confidence: 99%

“…Phenotyping was generally conducted at week 6 when inflorescences had produced between 20-25 flowers post anthesis. Floral organ abscission was quantified by counting the floral organs attached to the flowers in the main inflorescence as per (Alling and Galindo-Trigo, 2023). The main inflorescence stem was shaken four times and the number of floral organs that remained attached to each floral position (P1-P20) was visually inspected.…”

Section: Floral Organ Retention Quantificationmentioning

confidence: 99%

“…IDL1 promoter activity was detected in the secession cells only, while in the case of IDL2 and IDL3, promoter activities were detected in both residuum and secession cell layers (Figure 5D). Gene-edited knock-out lines for IDL2 and IDL3 were generated in the Col-0 background with the CRISPR-Cas9 technology, and higher order mutants were obtained by genetic crosses with previously published idaCR and idl1CR (Shi et al, 2018;Alling and Galindo-Trigo, 2023). The idaCR line was used despite the availability of the ida-2 allele, also in Col-0, due to T-DNA-induced genomic rearrangements in the ida-2 line that impede obtaining double mutants between ida and idl1 alleles (Alling and Galindo-Trigo, 2023).…”

Section: Overexpression Of Wrky57 or A Wrky57 Repressor Respectively ...mentioning

confidence: 99%

“…Gene-edited knock-out lines for IDL2 and IDL3 were generated in the Col-0 background with the CRISPR-Cas9 technology, and higher order mutants were obtained by genetic crosses with previously published idaCR and idl1CR (Shi et al, 2018;Alling and Galindo-Trigo, 2023). The idaCR line was used despite the availability of the ida-2 allele, also in Col-0, due to T-DNA-induced genomic rearrangements in the ida-2 line that impede obtaining double mutants between ida and idl1 alleles (Alling and Galindo-Trigo, 2023). Developing siliques in the quadruple mutant idaCR idl1CR idl2CR idl3CR retained floral organs more strongly than the single ida mutant, confirming the genetic redundancy between these IDL peptides as positive regulators of abscission (Figure 5E, F and Supplemental Figure S12).…”

Section: Overexpression Of Wrky57 or A Wrky57 Repressor Respectively ...mentioning

confidence: 99%

“…Double mutants hae hsl2 retain all floral organs across floral positions in the inflorescence (Cho et al, 2008;Stenvik et al, 2008). Meanwhile, ida knockouts display a weaker abscission phenotype, with floral organs being loosely attached in floral positions in which siliques are elongating (Stenvik et al, 2008;Alling and Galindo-Trigo, 2023). The weaker abscission phenotype in ida mutants is likely due to functional redundancy with related IDA-like (IDL) peptides in AZs (Stenvik et al, 2008;Vie et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

Galindo-Trigo,

Bågman,

Ishida

et al. 2023

Preprint

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Plants shed organs like leaves, petals or fruits through the process of abscission. Monitoring cues like age, resource availability, biotic and abiotic stresses allows plants to abscise organs in a timely manner. How these signals are integrated in the molecular pathways that drive abscission is largely unknown. The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene is one of the key regulators of floral organ abscission in Arabidopsis and is known to transcriptionally respond to most abscission-regulating cues. Interrogating the IDA promoter in silico and in vitro we identified transcription factors that can potentially modulate IDA expression. We functionally characterized the importance of ERF and WRKY binding sites for IDA expression during floral organ abscission. WRKY signaling is of special relevance to mediate IDA upregulation in response to biotic stress in tissues destined for separation. We further characterized WRKY57 as a potential positive regulator of IDA and IDA-like gene expression in abscission zones. Our findings highlight the promise of promoter element-targeted approaches to modulate the responsiveness of the IDA signaling pathway to harness abscission timing for improved crop productivity.

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Section: Plant Material Growth Conditions and Treatmentsmentioning

confidence: 99%

Section: Floral Organ Retention Quantificationmentioning

confidence: 99%

Section: Overexpression Of Wrky57 or A Wrky57 Repressor Respectively ...mentioning

confidence: 99%

Section: Overexpression Of Wrky57 or A Wrky57 Repressor Respectively ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

Galindo-Trigo,

Bågman,

Ishida

et al. 2023

Preprint

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A multifaceted kinase axis regulates plant organ abscission through conserved signaling mechanisms

Galindo-Trigo,

Khandare,

Roosjen

et al. 2024

Current Biology

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Dissection of theIDApromoter identifies WRKY transcription factors as abscission regulators in Arabidopsis

Galindo-Trigo,

Bågman,

Ishida

et al. 2024

Journal of Experimental Botany

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Plants shed organs like leaves, petals or fruits through the process of abscission. Monitoring cues like age, resource availability, biotic and abiotic stresses allows plants to abscise organs in a timely manner. How these signals are integrated in the molecular pathways that drive abscission is largely unknown. The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene is one of the main drivers of floral organ abscission in Arabidopsis and is known to transcriptionally respond to most abscission-regulating cues. Interrogating the IDA promoter in silico and in vitro we identified transcription factors that can potentially modulate IDA expression. We probed the importance of ERF and WRKY binding sites for IDA expression during floral organ abscission, with WRKYs being of special relevance to mediate IDA upregulation in response to biotic stress in tissues destined for separation. We further characterized WRKY57 as a positive regulator of IDA and IDA-like gene expression in abscission zones. Our findings highlight the promise of promoter element-targeted approaches to modulate the responsiveness of the IDA signaling pathway to harness controlled abscission timing for improved crop productivity.

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Reproductive defects in the abscission mutant ida-2 are caused by T-DNA–induced genomic rearrangements

Cited by 5 publications

References 33 publications

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

ERF and WRKY transcription factors regulateIDAand abscission timing in Arabidopsis

A multifaceted kinase axis regulates plant organ abscission through conserved signaling mechanisms

Dissection of theIDApromoter identifies WRKY transcription factors as abscission regulators in Arabidopsis

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