efficacy of the β-adrenergic receptor blocker (carazolol) at timed artificial insemination (tai) was investigated. cows (n = 73) were pre-synchronized with two PGF 2α injections given 14 d apart to initiate the Ovsynch protocol at early and middle luteal stages 14 d later, and received injections of GnrH and PGF 2 seven d apart followed by GnrH 48 h later, and tai 16 -18 h later. corpus luteum (cl) was detected via rectal palpation at the beginning of the Ovsynch protocol. carazolol (treatment i; n = 41) or physiologic saline (treatment ii; n = 32) were administered via jugular vein five min before tai. Uterine tone was determined prior to infusion and at tai via rectal palpation. Pregnancies were diagnosed with transrectal ultrasonography 40 ± 7 d after tai. Uterine tone was 2.8 (1.4 -5.3) times higher (P < 0.01) in treatment i than that in treatment ii at tai. increase in uterine tone was affected by treatment × cl × parity (P < 0.05), and 66.7%, 75%, 52.6%, 100% and 16.7%, 33.3%, 25%, 28.6% in primiparous cows with cl and without cl, and in multiparous cows with cl and without cl in treatments i and ii, respectively. Pregnancy rates did not differ between treatments i (34.2%) and ii (40.6%). in conclusion, no beneficial effect of carazolol administration prior to tai was found except for the increased uterine tone.