Reproductive potential of crimson‐spotted rainbowfish (Melanotaenia fluviatilis) following short‐term exposure to bass strait crude oil and dispersed crude oil

Abstract

An examination of the potential of crude oil and mixtures of dispersants and crude oil to act as reproductive toxicants is reported in this article. The short-term effects of a water-accommodated fraction of crude oil (WAF) and a dispersed crude oil water-accommodated fraction (DCWAF) on selected reproductive end points were measured by conducting 3-day exposures to the crimson-spotted rainbowfish (Melanotaenia fluviatilis). Exposures were followed by 14-day depuration periods to determine the ability of fish to recover from the exposure. There were no changes to egg production, hatchability, or larval lengths for the WAF and DCWAF test periods. There were no changes to plasma estradiol or testosterone concentrations, gonadosomatic indices, or histopathological organization of gonad tissues after the exposure and depuration periods for both WAF and DCWAF. As reproductive parameters were not altered after 3 days of exposure and 14 days of depuration, crimson-spotted rainbowfish were able to endure short-term exposures to crude oil and dispersed crude oil.

Hydrocarbon‐induced changes to metabolic and detoxification enzymes of the Australian crimson‐spotted rainbowfish (Melanotaenia fluviatilis)

“…It has been hypothesised that induction of CYP1A1 in sexually maturing fish may be linked to adverse reproductive effects by disrupting estrogen-regulated processes (Anderson et al, 1996). DCWAF exposure has the potential to reduce egg production (Pollino and Holdway, 2002).…”

Use of the australian crimson-spotted rainbowfish (Melanotaenia fluviatilis) as a model test species for investigating the effects of endocrine disruptors

“…To prevent fungal growth, the eggs were dipped in 4 mg/L of malachite green for 5 min [12]. These were replaced daily.…”

“…The eggs spawned over the 24 h were collected and counted. To prevent fungal growth, the eggs were dipped in 4 mg/L of malachite green for 5 min [12]. Eggs were distributed to petri dishes and placed in a 25ЊC incubator.…”

“…This species is ideal for measuring the effects of xenobiotics and has been used previously in ecotoxicological studies [12,13]. This species is ideal for measuring the effects of xenobiotics and has been used previously in ecotoxicological studies [12,13].…”

“…The crimson-spotted rainbowfish is an ideal test species because they are sexually dimorphic and can reproduce at approximately three months of age. This species is ideal for measuring the effects of xenobiotics and has been used previously in ecotoxicological studies [12,13]. In addition, a baseline study of the reproductive cycle of M. fluviatilis has been conducted previously [14].…”