Reproductive stage- and season-dependent culture characteristics of enriched caprine male germline stem cells

Singh, Shiva P.; Kharche, S. D.; Pathak, Manisha; Soni, Yogesh; Ranjan, Ravi; Singh, MK; Chauhan, M. S.

doi:10.1007/s10616-021-00515-x

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Total viable cells in each well were counted each day over 5 days period (by trypsinizing) by automatic cell counter (Countess II FL Automated Cell Counter; ThermoFisher Scientific). The total cell count was calculated to obtain the growth curve and PDT with the use of an online algorithm, PDT during culture for certain time intervals (d 0–3 and d 3–5) ( http://doubling-time.com ) 35 .…”

Section: Methodsmentioning

confidence: 99%

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells

Pathak,

Singh,

Kharche

et al. 2023

Sci Rep

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The enhanced availability of functional fibroblasts from precious tissue samples requires an ideal cell-culture system. Therefore, this study was designed to investigate the performance of caprine adult fibroblast cells (cadFibroblast) when cultivated in different culture media. The cadFibroblast cell lines from adult Barbari (Capra hircus) bucks were established and the effect of different media viz. DMEM/F-12 [with low-glucose (5.5 mM; DL) and high-glucose (30 mM; DH)], α-MEM [with low-glucose (5.5 mM; ML) and with high-glucose (30 mM; MH)], and fibroblast growth medium (FGM) were evaluated. Cells were then compared for growth characteristics and in-vitro dynamics through cellular morphology, proliferation, population-doubling time, double-immunocytochemistry, colony-forming units, wound healing, transwell migration, and differential expression of fibroblast-specific markers (FSP-1 and vimentin). The results of immunocytochemistry, transwell migration/invasion, and wound healing assays showed the superiority of DH over DL and other media tested. Whereas, similar effects of glucose supplementation and expression of FSP-1 were not observed in α-MEM. Transwell migration was significantly (p < 0.05) lower in FGM compared with other media tested. Overall, our results illustrate the media-dependent deviation in in-vitro dynamics and culture characteristics of cadFibroblasts that may be useful to develop strategies to cultivate these cells efficiently for research and downstream applications.

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Section: Methodsmentioning

confidence: 99%

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells

Pathak,

Singh,

Kharche

et al. 2023

Sci Rep

Self Cite

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show abstract

Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo ( Bubalus bubalis ) testes

Carlo,

Pathak,

Choudhary

et al. 2023

Animal Biotechnology

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Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells

Pathak

Singh

Kharche

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The enhanced availability of functional fibroblasts from precious tissue samples requires an ideal cell-culture system. Therefore, this study was designed to investigate the performance of caprine adult fibroblast cells (cadFibroblast) when cultivated in different culture media. The cadFibroblast cell lines from adult Barbari bucks were established and the effect of different media viz. DMEM/F-12 [with low glucose (5.5 mM; DL) and high glucose (30 mM; DH)], α-MEM [with low glucose (5.5 mM; ML) and with high glucose (30 mM; MH)], and fibroblast growth medium (FGM) were evaluated and compared for growth characteristics and in-vitro dynamics through cellular morphology, proliferation, population doubling time, double-immunocytochemistry, colony forming units, wound healing, transwell migration and differential expression of fibroblast-specific markers [FSP-1 and vimentin]. The results of immunocytochemistry, transwell migration/invasion, and wound healing assays showed the superiority of DH over DL and other media tested. Whereas, similar effects of glucose supplementation and expression of FSP-1 were not observed in α-MEM. Transwell migration was significantly (p<0.05) lower in FGM compared with other media tested. Overall, our results illustrate the media-dependent deviation in in-vitro dynamics and culture characteristics of cadFibroblasts that may be useful to develop strategies to cultivate these cells efficiently for research and downstream applications.

show abstract

Reproductive stage- and season-dependent culture characteristics of enriched caprine male germline stem cells

Cited by 3 publications

References 45 publications

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells

Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo ( Bubalus bubalis ) testes

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells

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