Reproductive System Symbiotic Bacteria Are Conserved between Two Distinct Populations of
            <i>Euprymna scolopes</i>
            from Oahu, Hawaii

Kerwin, Allison H.; Nyholm, Spencer V.

doi:10.1128/msphere.00531-17

Cited by 15 publications

(25 citation statements)

References 34 publications

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“…1A and B) (12). The ANG community is composed predominantly of Alphaproteobacteria , Gammaproteobacteria , and Verrucomicrobia , depending on the cephalopod species (13–18). These bacteria are added to the egg jelly coat (JC; Fig.…”

Section: Introductionmentioning

confidence: 99%

“…scolopes is well known for its association with the light organ symbiont Vibrio fischeri and, more recently, for its ANG consortium (17, 25). The host is easily maintained in the laboratory with minimal effect on the composition of the ANG bacterial community (16, 17), which is dominated by Alphaproteobacteria from the Rhodobacteraceae , as well as Verrucomicrobia , with some Flavobacteriia and Gammaproteobacteria (16–18). The ease of maintaining the host in the laboratory and the stability of its ANG community in captivity and throughout embryogenesis (17) make this system ideal for investigating the function of ANG bacteria in cephalopods.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Shielding the Next Generation: Symbiotic Bacteria from a Reproductive Organ Protect Bobtail Squid Eggs from Fungal Fouling

Kerwin

Gromek

Suria

et al. 2019

mBio

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Organisms must have strategies to ensure successful reproduction. Some animals that deposit eggs protect their embryos from fouling/disease with the help of microorganisms. Although beneficial bacteria are hypothesized to contribute to egg defense in some organisms, the mechanisms of this protection remain largely unknown, with the exception of a few recently described systems. Using both experimental and analytical approaches, we demonstrate that symbiotic bacteria associated with a cephalopod reproductive gland and eggs inhibit fungi. Chemical analyses suggest that these bacteria produce antimicrobial compounds that may prevent overgrowth from fungi and other microorganisms. Given the distribution of these symbiotic glands among many cephalopods, similar defensive relationships may be more common in aquatic environments than previously realized. Such defensive symbioses may also be a rich source for the discovery of new antimicrobial compounds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shielding the Next Generation: Symbiotic Bacteria from a Reproductive Organ Protect Bobtail Squid Eggs from Fungal Fouling

Kerwin

Gromek

Suria

et al. 2019

mBio

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“…Across both vertebrates and invertebrates, there are many examples of vertical transmission of microbiomes. Demonstrated examples include insects [68,69], sponges [70], bivalves [71,72,73], and cephalopods [74,75,76,77]. In sponges, members of up to ten bacterial phyla and one archael phylum have been vertically transmitted from adult sponge to other life stages, including oocytes of oviparous sponges and embryos of viviparous sponges [78].…”

Section: Fetal Vs Adultmentioning

confidence: 99%

Characterization of the Gut Microbiome in Wild Rocky Mountainsnails (Oreohelix strigosa)

Chalifour

2021

Preprint

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BackgroundThe Rocky Mountainsnail (Oreohelix strigosa) is a terrestrial gastropod of ecological importance in the Rocky Mountains of western United States and Canada. Across the animal kingdom, including in gastropods, gut microbiomes have profound effects on the health of the host. Current knowledge regarding snail gut microbiomes, particularly throughout various life history stages, is limited. Understanding snail gut microbiome composition and dynamics can provide an initial step toward better conservation and management of this species. ResultsIn this study, we employed 16S rRNA gene amplicon sequencing to examine gut bacteria communities in wild-caught O. strigosa populations from the Front Range of Colorado. These included three treatment groups: (1) adult and (2) fetal snails, as well as (3) sub-populations of adult snails that were starved prior to ethanol fixation. Overall, O. strigosa harbors a high diversity of bacteria. We sequenced the V4 region of the 16S rRNA gene on an Illumina MiSeq, and obtained 2,714,330 total reads. We identified a total of 7,056 unique operational taxonomic units (OTUs) belonging to 36 phyla. The core gut microbiome of four unique OTUs accounts for roughly half of all sequencing reads returned and may aid the snails’ digestive processes. Significant differences in microbial composition, as well as richness, evenness, and Shannon Indices were found across the three treatment groups.ConclusionsComparisons of gut microbiomes in O. strigosa adult, fetal, and starved samples provide evidence that the host internal environments influence bacterial community compositions, and that bacteria may be transmitted vertically from parent to offspring. This work provides the first comprehensive report on the structure and membership of bacterial populations in the gastropod family Oreohelicidae and reveals similarities and differences across varying life history metrics. Strong differentiation between these life history metrics demonstrates the need for wider sampling for studies of dynamics of the snail gut microbiome.

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“…B 375: 20190593 light organ symbiont Vibrio fischeri [66,67]. Recent work has also investigated the ANG consortium, describing the bacterial community and its function [41,61,[68][69][70]. In E. scolopes, the ANG contains mainly Alphaproteobacteria and Verrucomicrobia, along with members of the Gammaproteobacteria and Flavobacteriia [61,69].…”

Section: (B) Defensive Symbiosesmentioning

confidence: 99%

In the beginning: egg–microbe interactions and consequences for animal hosts

Nyholm

2020

Phil. Trans. R. Soc. B

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Microorganisms are associated with the eggs of many animals. For some hosts, the egg serves as the ideal environment for the vertical transmission of beneficial symbionts between generations, while some bacteria use the egg to parasitize their hosts. In a number of animal groups, egg microbiomes often perform other essential functions. The eggs of aquatic and some terrestrial animals are especially susceptible to fouling and disease since they are exposed to high densities of microorganisms. To overcome this challenge, some hosts form beneficial associations with microorganisms, directly incorporating microbes and/or microbial products on or in their eggs to inhibit pathogens and biofouling. Other functional roles for egg-associated microbiomes are hypothesized to involve oxygen and nutrient acquisition. Although some egg-associated microbiomes are correlated with increased host fitness and are essential for successful development, the mechanisms that lead to such outcomes are often not well understood. This review article will discuss different functions of egg microbiomes and how these associations have influenced the biology and evolution of animal hosts. This article is part of the theme issue ‘The role of the microbiome in host evolution’.

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Reproductive System Symbiotic Bacteria Are Conserved between Two Distinct Populations of Euprymna scolopes from Oahu, Hawaii

Cited by 15 publications

References 34 publications

Shielding the Next Generation: Symbiotic Bacteria from a Reproductive Organ Protect Bobtail Squid Eggs from Fungal Fouling

Shielding the Next Generation: Symbiotic Bacteria from a Reproductive Organ Protect Bobtail Squid Eggs from Fungal Fouling

Characterization of the Gut Microbiome in Wild Rocky Mountainsnails (Oreohelix strigosa)

In the beginning: egg–microbe interactions and consequences for animal hosts

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