Reproductive toxicities of 1-ethyl-3-methylimidazolium bromide on Caenorhabditis elegans with oscillation between inhibition and stimulation over generations

Yue, Wanyan; Mo, Ling-Yun; Zhang, Jing

doi:10.1016/j.scitotenv.2020.144334

Cited by 39 publications

(25 citation statements)

References 46 publications

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“…Several studies indicate that ILs have toxic effects on the reproductive system and development in offspring 40–43 . In this study, there was a slight decrease in the number of mature sperm in parents exposed to [C n mim]NO 3 , indicating that [C n mim]NO 3 possibly delays or inhibits spermatogenesis.…”

Section: Discussionmentioning

confidence: 44%

“…38 Several studies indicate that ILs have toxic effects on the reproductive system and development in offspring. [40][41][42][43] In this study, there was a slight decrease in the number of mature sperm in parents Some of the upregulated DEGs included cpa5 and col1a1a were enriched in the bone mineralization involved in bone maturation and extracellelar space, which may result in abnormal development. The zebrafish cpa5 has been identified to be a mast cell-specific marker and regulate bone production and resorption.…”

Section: Discussionmentioning

confidence: 58%

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Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Liu

et al. 2023

Environmental Toxicology

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Ionic liquids (ILs) are thought to have negative effects on human health. Researchers have explored the effects of ILs on zebrafish development during the early stages, but the intergenerational toxicity of ILs on zebrafish development has rarely been reported. Herein, parental zebrafish were exposed to different concentrations (0, 12.5, 25, and 50 mg/L) of [Cnmim]NO3 (n = 2, 4, 6) for 1 week. Subsequently, the F1 offspring were cultured in clean water for 96 h. [Cnmim]NO3 (n = 2, 4, 6) exposure inhibited spermatogenesis and oogenesis in F0 adults, even causing obvious lacunae in the testis and atretic follicle oocytes in ovary. After parental exposure to [Cnmim]NO3 (n = 2, 4, 6), the body length and locomotor behavior were measured in F1 larvae at 96 hours post‐fertilization (hpf). The results showed that the higher the concentration of [Cnmim]NO3 (n = 2, 4, 6), the shorter the body length and swimming distance, and the longer the immobility time. Besides, a longer alkyl chain length of [Cnmim]NO3 had a more negative effect on body length and locomotor behavior. RNA‐seq analysis revealed several downregulated differentially expressed genes (DEGs)—grin1b, prss1, gria3a, and gria4a—enriched in neurodevelopment‐related pathways, particularly the pathway for neuroactive ligand–receptor interaction. Moreover, several upregulated DEGs, namely col1a1a, col1a1b, and acta2, were mainly associated with skeletal development. Expression of DEGs was tested by RT‐qPCR, and the outcomes were consistent with those obtained from RNA‐Seq. We provide evidence showing the effects of parental exposure to ILs on the regulation of nervous and skeletal development in F1 offspring, demonstrating intergenerational effects.

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Section: Discussionmentioning

confidence: 44%

Section: Discussionmentioning

confidence: 58%

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Liu

et al. 2023

Environmental Toxicology

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“…Such compensatory response to toxins has been observed in C. elegans , where increased histone methylation was proposed to play a role (Yue et al. 2021 ). Because the methylation and phenotypic experiments were carried out separately, we cannot directly link the observed long‐lasting epigenetic changes to significant life history effects.…”

Section: Discussionmentioning

confidence: 78%

“…Although it is difficult to speculate on the cause of these phenotypic effects, we note that size at maturity was reduced in directly affected F0, with apparent compensatory in the F3. Such compensatory to toxins has been observed in C. elegans, where increased histone methylation was proposed to a role (Yue et al 2021). Because the methylation and phenotypic experiments were carried out separately, we cannot directly link the long-lasting epigenetic changes to significant life history effects.…”

Section: Phenotypic and Evolutionary Consequencesmentioning

confidence: 95%

Pollution induces epigenetic effects that are stably transmitted across multiple generations

et al. 2022

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It has been hypothesized that the effects of pollutants on phenotypes can be passed to subsequent generations through epigenetic inheritance, affecting populations long after the removal of a pollutant. But there is still little evidence that pollutants can induce persistent epigenetic effects in animals. Here, we show that low doses of commonly used pollutants induce genome‐wide differences in cytosine methylation in the freshwater crustacean Daphnia pulex. Uniclonal populations were either continually exposed to pollutants or switched to clean water, and methylation was compared to control populations that did not experience pollutant exposure. Although some direct changes to methylation were only present in the continually exposed populations, others were present in both the continually exposed and switched to clean water treatments, suggesting that these modifications had persisted for 7 months (>15 generations). We also identified modifications that were only present in the populations that had switched to clean water, indicating a long‐term legacy of pollutant exposure distinct from the persistent effects. Pollutant‐induced differential methylation tended to occur at sites that were highly methylated in controls. Modifications that were observed in both continually and switched treatments were highly methylated in controls and showed reduced methylation in the treatments. On the other hand, modifications found just in the switched treatment tended to have lower levels of methylation in the controls and showed increase methylation in the switched treatment. In a second experiment, we confirmed that sublethal doses of the same pollutants generate effects on life histories for at least three generations following the removal of the pollutant. Our results demonstrate that even low doses of pollutants can induce transgenerational epigenetic effects that are stably transmitted over many generations. Persistent effects are likely to influence phenotypic development, which could contribute to the rapid adaptation, or extinction, of populations confronted by anthropogenic stressors.

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“…Furthermore, due to the fact that ILs are purely ionic compounds, there is the possibility to design an enormous number of cation and anion combinations with different, specific properties. Although they have been hailed as "green solvents" not long ago, significant drawbacks, related mainly to their toxicity and persistence, have already been noticed (Samori et al 2007;Steudte et al 2014;Steudte et al 2012;Stolte et al 2008;Jeremias et al 2021;Yue et al 2021;Mena et al 2020;Khan et al 2021). However, data on their potential for accumulation in living organisms are still limited (Kowalska et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Interaction of ionic liquids with human serum albumin in the view of bioconcentration: a preliminary study

et al. 2022

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Bioaccumulation potential is critical in PBT and risk assessment of chemicals. However, for ionic liquids (ILs), this aspect remains neglected. It is especially important to fill this gap, because for this group of compounds, existing data confirm their risk of being environmentally persistent and toxicity. Moreover, considering preliminary reports on the interactions of ILs with lipids, it may be assumed that ILs have a higher potential for bioaccumulation than indicated by previous estimations built upon octanol–water partition coefficients. Moreover, the bioconcentration of ionizable chemical compounds may also be strongly related to plasma protein contents. Therefore, in this work, the affinity of a set of imidazolium cations and organic anions, and their combination to human serum albumin (HSA) was determined. The obtained results reveal that both cations and anions can be strongly bound to HSA, and blood proteins might play an important role in overall bioaccumulation. Furthermore, it was observed that HSA binding properties towards IL cations depend on the hydrophobicity of cations. The obtained data also provide indication that cation–anion interaction may affect ILs ions affinity to HSA.

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Reproductive toxicities of 1-ethyl-3-methylimidazolium bromide on Caenorhabditis elegans with oscillation between inhibition and stimulation over generations

Cited by 39 publications

References 46 publications

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Pollution induces epigenetic effects that are stably transmitted across multiple generations

Interaction of ionic liquids with human serum albumin in the view of bioconcentration: a preliminary study

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Reproductive toxicities of 1-ethyl-3-methylimidazolium bromide on Caenorhabditis elegans with oscillation between inhibition and stimulation over generations

Cited by 39 publications

References 46 publications

Intergenerational toxic effects of parental exposure to [Cnmim]NO3 (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Intergenerational toxic effects of parental exposure to [Cnmim]NO3 (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Pollution induces epigenetic effects that are stably transmitted across multiple generations

Interaction of ionic liquids with human serum albumin in the view of bioconcentration: a preliminary study

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Product

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Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring