Reproductive toxicity of heavy metals in fallow deer in vitro

Abdel-Salam, E; Banďouchová, Hana; Heger, Tomáš; Kaňová, Miroslava; Kobelková, Kateřina; Němcová, Monika; Piaček, Vladimír; Sedláčková, Jana; Seidlová, Veronika; Vitula, F.; Pikula, Jiří

doi:10.2754/avb202190030277

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…The authors determined the lowest observed adverse effect concentration (LOAEC) for each metal and reported it to be 25 µM for CdCl2 and 100 µM for PbCl2 (59). Another study tested the adverse effects of CdCl2, MeHgCl2 and PbCl2 through a variety of equal molar concentrations (0, 15, 30, 60, 125, 250 µM) (67).…”

Section: Heavy Metals Caused a Limited Increase In Ros Productionmentioning

confidence: 99%

Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line

Strijp

Rooy

Serem

et al. 2023

Ultrastructural Pathology

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Heavy metals are natural elements characterized by their relatively large atomic mass as well as their high density. Heavy metal poisoning has occurred in numerous countries in the past, with the United States having the highest prevalence. Heavy metals can also be introduced into the ecosystem by the mining of heavy metals from deep within the earth's crust thereby exposing them into air and water systems.Another common source of heavy metal exposure is cigarette smoke. Cigarette smoke has been shown to have carcinogenic, toxic and genotoxic properties. Although all the toxic elements in cigarette smoking have not yet been identified, several heavy metals have been found to contribute to the pathophysiological consequences associated with smoking. A study conducted by Yaprak et al., in 2019 indicated that cadmium, lead and chromium are the most abundant metals found in cigarette smoke. The current study therefore focused on cadmium, lead and chromium, alone and as part of metal mixtures to determine the role of these heavy metals on endothelial cells. The EA.hy926 endothelial cell line was exposed to different concentrations of each of these metal and their combinations and analysed at different time points to determine the effect of the heavy metals on endothelial cell function. The cytotoxicity of the metals was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Crystal Violet assays, reactive oxygen species productions was studied using the 2',7'dichlorodihydrofluorescein diacetate (DCFH-DA) assay, and cell viability was determined using flow cytometry. Finally, morphological changes caused by these metals was studied using scanning electron microscopy.Exposure of endothelial cells to cadmium, lead and chromium alone and in combination at three different concentrations (X0.1, X1 and X2) showed no significant cytotoxicity as indicated by the MTT and Crystal Violet assay results. At 24 h exposure, cadmium alone showed an increase in percentage free radical formation whereas lead alone showed the greatest percentage radical formation for the X1 concentration group. At 48 h exposure, chromium alone as well as the triple combination group showed an increase in the percentage radical formation between 0.1 and X2 concentrations. Cadmium caused the highest percentage radical formation in the X1 concentration group and lead at the X2 concentration group. After 72 h, both the cadmium and lead as single metals showed a gradual increase in percentage radical formation between the 0.1 and X2 concentrations, with cadmium showing the highest increase in the X1 concentration. Flow cytometric analyses with the Annexin V and the Propidium Iodide assay, showed an increase in early apoptotic and necrotic cells with higher concentrations of the lead and chromium © © U Un ni iv ve er rs si it ty y o of f P Pr re et to or ri ia a 4 combination as well as in the triple combination group. Increased necrosis was also evident in the cadmium and chromium combination, the lead and chromium as well...

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Section: Heavy Metals Caused a Limited Increase In Ros Productionmentioning

confidence: 99%

Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line

Strijp

Rooy

Serem

et al. 2023

Ultrastructural Pathology

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Natural mercury exposure of European insectivorous bats may exceed a recognized toxicity threshold

Bandouchova,

Novotna Kruzikova,

Zukal

et al. 2024

Ecotoxicology

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Heavy metals are an important group of toxic substances harmful for many organisms. Of these, mercury is one of the most monitored in the environment. Several matrices are used for the monitoring of environmental load, including a range of organisms; bats, however, have only been examined rarely. Insectivorous bats are apex predators threatened by several human interventions in their natural environment, including heavy metal pollution. The aim of this study was to analyze the content of total mercury in the fur, flight membrane, and pectoral muscle of greater mouse-eared bats (Myotis myotis). Total mercury concentrations were also measured in carabid beetles from the catch locality Zastávka u Brna. Samples were obtained from 43 bat carcasses at two different localities in the Czech Republic (Zastávka u Brna, Malá Morávka). Total mercury content varied between 1.76–72.20 µg/g in fur, 0.04–0.14 µg/g in skin, and 0.05–0.20 µg/g in muscle. Total mercury values in the fur of some individuals from Malá Morávka exceeded the recognized toxicity limit. Furthermore, there was a significant difference (p < 0.001) in content of total mercury in fur between localities, and there was a clear effect of age on concentrations in fur, skin, and muscle, the concentrations being significantly correlated (fur and skin rs = 0.783; fur and muscle rs = 0.716; skin and muscle rs = 0.884). These findings confirm the usefulness of fur samples from living bats for biomonitoring mercury burden in the environment.

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Analysis of Fibropapillomatosis in Roe Deer (Capreolus capreolus) Confirms High Content of Heavy Metals

Matějka Košinová,

Cukor,

Skoták

et al. 2024

Animals

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In recent decades, there has been an increase in European wild ungulate populations, often associated with a decline in health and spread of disease. This is true for the roe deer (Capreolus capreolus), the most common European cervid, with populations apparently affected by fibropapillomatosis, an increasingly common cancer. To date, however, there has been little research into this disease, thus many interactions remain unclear and descriptions of tumour composition are poorly validated. The main aim of the present study was to evaluate the presence and concentration of toxic heavy metals in roe deer skin tumours. Our results confirmed the presence of virtually all the metals tested for, i.e., Pb, Hg, Cd, As, Cr, Mn, Al, Co, Cu, Ni, Se, Zn, and Fe, with the highest average concentrations found for Cr (0.99 mg/kg−1 ± 2.23 SD), Cd (0.03 mg/kg−1 ± 0.03 SD), and Hg (0.02 mg/kg−1 ± 0.02 SD), exceeding FAO limits for meat from slaughtered animals. We also observed a significant positive relationship between heavy metal concentration and age, especially for Pb, As, Hg, Mn, Se, Al, Zn, and Ni. Our findings provide a strong baseline for further research on the impact of fibropapillomatosis, not only on the welfare and health status of game but also on the final consumer of venison, which in many respects is regarded as a high-quality, ecological, and renewable wild resource. While deer with this disease are not considered qualitatively or medically defective, they could represent a potential reservoir of substances toxic to humans and could affect substance levels in adjacent tissues or the animal as a whole.

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Reproductive toxicity of heavy metals in fallow deer in vitro

Cited by 3 publications

References 43 publications

Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line

Investigating the effect of the heavy metals cadmium, chromium and lead, alone and in combination on an endothelial cell line

Natural mercury exposure of European insectivorous bats may exceed a recognized toxicity threshold

Analysis of Fibropapillomatosis in Roe Deer (Capreolus capreolus) Confirms High Content of Heavy Metals

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