Reproductive Traits of Hermaphroditic Muscadine Cultivars

Biasi, Luiz Antônio; Conner, Patrick J.

doi:10.21273/hortsci.51.3.255

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…Expression of grape SISTER OF RA3 (VvSRA), also a TPP gene, is negatively related to pedicel development on the lateral branch of grape inflorescences, thereby decreasing berry number (Ishiai et al, 2015). Male and hermaphroditic muscadine inflorescences generally produce more flowers than do female inflorescences (Biasi and Conner, 2016;Detjen, 1917). Expression studies of the VR006 gene may help in determining if this gene could play a role in sex determination in muscadine.…”

Section: Discussionmentioning

confidence: 99%

Development and Characterization of Molecular Markers Associated with Female Plants in Muscadine Grape

Conner

Catotti

et al. 2017

J. Amer. Soc. Hort. Sci.

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Muscadine (Vitis rotundifolia Michx.) vines may be male (M), female (F), or hermaphroditic (H). Male flowers have only filaments and anthers, whereas female and hermaphroditic flowers are morphologically perfect. Female flowers are distinguished from hermaphroditic flowers by their reflexed stamens (as opposed to upright) and nonfunctional pollen. Primers derived from previously identified candidate genes located at the sex locus of Vitis vinifera L. were used to generate amplicons from M, F, and H muscadine cultivars. Sequence analysis of the amplicons revealed insertion/deletion (indel) polymorphisms in a trehalose-6-phosphate phosphatase (TPP) gene (VR006) and a WRKY transcription factor 21 gene (VR009). Primers were designed to create diagnostic markers for each indel polymorphism. Associations between the marker alleles and the plant sex trait were examined in a wide range of muscadine germplasm and in segregating populations derived from F × H, F × M, and H × H crosses. VR006 produced a codominant marker that was able to differentiate the female-associated allele from the male- and hermaphroditic-associated alleles. The marker was able to detect female plants and will be suitable for screening breeding program progenies. VR009 was able to detect the presence of the female allele in most germplasm, but a crossover event appears to have separated the marker from the sex locus in some germplasm. As shown in previous muscadine genetic studies, H × H-derived populations produced male seedlings. Marker analysis of these populations indicates that male flowers only occur in seedlings which are heterozygous for F- and H-associated marker alleles and inheritance of flower type in muscadine remains unclear.

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Section: Discussionmentioning

confidence: 99%