Republication – APD SpectBT: Arduino-based mobile vis-Spectrophotometer

Ng, Khairani Muhammad; Wong, Chun-Foong; Liang, Andre Xin; Liew, Ying-Heng; Yeo, Joshua Yi; Lua, Wai-Heng; Qian, Xi-Jun; Gan, Samuel Ken‐En

doi:10.30943/2019/s23122019

Cited by 7 publications

(7 citation statements)

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“…Such superantigen-based diagnostics can be coupled with colorimetric, home-made devices [e.g. mobile spectrophotometers ( 152 , 153 )]. Given the increasing association of antibody V H families with certain diseases e.g.…”

Section: Clinical Usagementioning

confidence: 99%

Superantigen Recognition and Interactions: Functions, Mechanisms and Applications

2021

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Superantigens are unconventional antigens which recognise immune receptors outside their usual recognition sites e.g. complementary determining regions (CDRs), to elicit a response within the target cell. T-cell superantigens crosslink T-cell receptors and MHC Class II molecules on antigen-presenting cells, leading to lymphocyte recruitment, induction of cytokine storms and T-cell anergy or apoptosis among many other effects. B-cell superantigens, on the other hand, bind immunoglobulins on B-cells, affecting opsonisation, IgG-mediated phagocytosis, and driving apoptosis. Here, through a review of the structural basis for recognition of immune receptors by superantigens, we show that their binding interfaces share specific physicochemical characteristics when compared with other protein-protein interaction complexes. Given that antibody-binding superantigens have been exploited extensively in industrial antibody purification, these observations could facilitate further protein engineering to optimize the use of superantigens in this and other areas of biotechnology.

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Section: Clinical Usagementioning

confidence: 99%

Superantigen Recognition and Interactions: Functions, Mechanisms and Applications

2021

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“…All students extracted the original pET21d plasmid (Invitrogen, Singapore) from in‐house competent Escherichia coli DH5α strain, grown in Luria‐Bertani broth supplemented with 100 μg/ml of ampicillin, cultured at 37°C, 250 rpm for 15 h before each run. At the final step of the mini‐prep procedure, they eluted the plasmid in 30 μl of elution buffer before the yield and purity were measured using the Nanodrop1000 Spectrophotometer (ThermoFisher Scientific, Singapore) and a mobile spectrophotometer 15,16 . The mini‐prep yield concentration in ng/μl and purity by A 260/280 were the parameters for analysis.…”

Section: Methodsmentioning

confidence: 99%

Effects of different delivery modes on teaching biomedical science practical skills in higher education during the 2021 pandemic measures

Heng

Koh

Yeo

et al. 2022

Biochem Molecular Bio Educ

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The COVID-19 pandemic related measures had augmented the rise of online education. While online teaching had mitigated the negative impacts from educational institutional closures, it was unable to displace hands-on biomedical laboratory practical lessons effectively. Without practical sessions, there was concern over the imparting of laboratory skills even with video demonstrations. To investigate the effectiveness of different delivery modes in imparting laboratory skills, theoretical and practical student assessments were analyzed alongside an anonymous survey on their motivation and prior experience. The undergraduate students were exposed to (1) instructor-live demonstration; (2) video demonstration or (3) no demonstration prior to the practical test which was a plasmid extraction. Significantly higher mini-prep yields and purity were found for both instructor-live and video demonstrations compared to no demonstration. Comparison with pre-pandemic theoretical assessment performance showed no significant differences despite longer contact hours during pre-pandemic times. Prior lab experience and motivation for selecting the course did not significantly affect student mini-prep yields. In conclusion, our findings suggest that video demonstrations were as effective as instructorlive demonstrations during the pandemic without noticeably compromising the teaching and learning of biomedical laboratory skills.

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“…The bacterial cultures were diluted 5-fold at required time intervals before OD 600 measurements were taken using IMPLEN Nanophotometer P330 and APD SpectBT. 22 LB with ampicillin was used as blank. Plasmids were extracted at Schematic diagram outlining the processes of the plasmid extraction study and their timelines.…”

Section: Od Measurement Plasmid Miniprep and Plasmid Quantificationmentioning

confidence: 99%

To Plate or to Simply Unfreeze, That Is the Question for Optimal Plasmid Extraction

Thean¹,

Ong²,

Heng³

et al. 2021

J Biomol Tech

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Many molecular biology applications require fast plasmid DNA extraction, spurring multiple studies on how to speed up the process. It is regularly instructed in standard laboratory protocols to plate out frozen glycerol bacterial stocks prior to bacteria incubation in liquid media and subsequent plasmid extraction, although the rationale for this is often unexplained (other than for the isolation of single colonies). Given the commonality and importance of this laboratory operation, such a practice is time-consuming and laborious. To study the impact of this practice and the alternative direct culturing method, we investigated the association between bacterial cell mass and its potential influence on plasmid yields from the 2 methods. Our results showed no difference with preplating for 7 out of 8 plasmid constructs used in the study, suggesting that direct glycerol recovery would not lead to poorer plasmid yields. The findings support the rationale for direct glycerol recovery for plasmid extraction, without the need of an intermediate preplating step.

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Republication – APD SpectBT: Arduino-based mobile vis-Spectrophotometer

Cited by 7 publications

References 0 publications

Superantigen Recognition and Interactions: Functions, Mechanisms and Applications

Superantigen Recognition and Interactions: Functions, Mechanisms and Applications

Effects of different delivery modes on teaching biomedical science practical skills in higher education during the 2021 pandemic measures

To Plate or to Simply Unfreeze, That Is the Question for Optimal Plasmid Extraction

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