Repulsive effect of salt on solvent extraction of 2,3-butanediol from aqueous fermentation solution

Birajdar, Snehal D.; Padmanabhan, Sasisanker; Rajagopalan, S.

doi:10.1002/jctb.4450

Cited by 24 publications

(19 citation statements)

References 38 publications

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“…A similar recovery method is repulsive extraction, also known as salt-assisted solvent extraction, and this method is applicable to a wide range of fermentation-derived biochemicals [100,101]. The approach involves selectively reducing the solubility of the desired product.…”

Section: Downstream Processesmentioning

confidence: 99%

Recent Progress in the Microbial Production of Pyruvic Acid

Maleki

Eiteman

2017

Fermentation

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Pyruvic acid (pyruvate) is a cellular metabolite found at the biochemical junction of glycolysis and the tricarboxylic acid cycle. Pyruvate is used in food, cosmetics, pharmaceutical and agricultural applications. Microbial production of pyruvate from either yeast or bacteria relies on restricting the natural catabolism of pyruvate, while also limiting the accumulation of the numerous potential by-products. In this review we describe research to improve pyruvate formation which has targeted both strain development and process development. Strain development requires an understanding of carbohydrate metabolism and the many competing enzymes which use pyruvate as a substrate, and it often combines classical mutation/isolation approaches with modern metabolic engineering strategies. Process development requires an understanding of operational modes and their differing effects on microbial growth and product formation.

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Section: Downstream Processesmentioning

confidence: 99%

Recent Progress in the Microbial Production of Pyruvic Acid

Maleki

Eiteman

2017

Fermentation

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“…A greater salting-out effect of the salting-out agent was observed when it was used in the fermentation broth rather than in the aqueous solution, due to the presence of salt and solutes in the fermentation broth. 25 The effect of organic solvent type on the recovery of isobutanol at 298.15 K was investigated, as shown in Fig. 6.…”

Section: The Recovery Of Isobutanolmentioning

confidence: 99%

“…2, 3-butanediol (2, 3-BD) was separated from synthetic solution and fermentation broth by using K 2 HPO 4 as salting-out agent, and 2-sec butyl phenol and 1-butanol as extractants. 25 Compared with salt free extraction, a 1.5-3-fold increase in distribution coefficient and 7-14-fold increase in separation factor were achieved. The recovery of 1, 3-propanediol (1, 3-PD) from fermentation broth by SOE was investigated, and results showed that the recovery of 1, 3-PD was higher than 90% and most impurities were removed from the organic phase.…”

Section: Introductionmentioning

confidence: 95%

“…The distribution coefficient and the selectivity of acetone and 1‐butanol were markedly improved with the addition of salting‐out agent to the aqueous feed, but the recovery of target product, organic solvent and salt needs to be further improved. 2, 3‐butanediol (2, 3‐BD) was separated from synthetic solution and fermentation broth by using K 2 HPO 4 as salting‐out agent, and 2‐sec butyl phenol and 1‐butanol as extractants . Compared with salt free extraction, a 1.5–3‐fold increase in distribution coefficient and 7–14‐fold increase in separation factor were achieved.…”

Section: Introductionmentioning

confidence: 99%

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Salting‐out extraction of bio‐based isobutanol from an aqueous solution

Zhang

Xie

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND More efficient downstream separation technologies need to be explored due to the low concentration of bio‐based isobutanol in the fermentation broth. The salting‐out extraction of isobutanol from its aqueous solution by employing nine salts (K4P2O7·3H2O, K2HPO4·3H2O, K3PO4·3H2O, K2CO3, K2SO4, KCl, Na2CO3, Na2SO4, NaCl) as salting‐out agents and three organic solvents (2‐ethyl‐1‐hexanol, cyclopentanol, 2‐methyl‐2‐butanol) as extractants were investigated at 298.15 K. RESULTS The recovery was greatly influenced by the salt concentration and types of salts and organic solvents. When the initial molar K4P2O7 concentration was equal to or higher than 1.01 mol kg‐1 and the mass ratio of aqueous isobutanol solution to 2‐ethyl‐1‐hexanol was 1:1, the recovery of isobutanol reached 100%, and more than 91.62% of water was removed from the organic phase. CONCLUSION The salting‐out effects of different anions in nine salts were ordered in the sequence: Cl‐ < SO42‐ < HPO42‐ < CO32‐ < PO43‐ < P2O74‐ at the same initial molar salt concentration and using the same organic solvent. 2‐Ethyl‐1‐hexanol was verified to be the most suitable extractant. The distribution behavior and salting‐out extraction effects can be evaluated by tie‐line correlations and solubility correlation. © 2017 Society of Chemical Industry

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“…In fact, most products are initially produced intracellularly, but some products are localized extracellularly to the aqueous medium through passive diffusion or active transport [52]. Previous work on economic assessment for the separation of extracellular chemicals has been mainly restricted to specific examples such as hyaluronic acid [53][54][55][56][57], limonene [58][59][60][61], xanthan gum [62,63], butanediol [64][65][66][67], lactic acid [68][69][70][71][72] and penicillin V [19,73,74]. Also, assessment studies have been performed for individual separation technologies [75][76][77].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Yenkie

Maravelias

2019

BMC Chem Eng

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Recent advances in metabolic engineering have enabled the production of chemicals via bio-conversion using microbes. However, downstream separation accounts for 60-80% of the total production cost in many cases. Previous work on microbial production of extracellular chemicals has been mainly restricted to microbiology, biochemistry, metabolomics, or techno-economic analysis for specific product examples such as succinic acid, xanthan gum, lycopene, etc. In these studies, microbial production and separation technologies were selected apriori without considering any competing alternatives. However, technology selection in downstream separation and purification processes can have a major impact on the overall costs, product recovery, and purity. To this end, we apply a superstructure optimization based framework that enables the identification of critical technologies and their associated parameters in the synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions. We divide extracellular chemicals into three categories based on their physical properties, such as water solubility, physical state, relative density, volatility, etc. We analyze three major extracellular product categories (insoluble light, insoluble heavy and soluble) in detail and provide suggestions for additional product categories through extension of our analysis framework. The proposed analysis and results provide significant insights for technology selection and enable streamlined decision making when faced with any microbial product that is released extracellularly. The parameter variability analysis for the product as well as the associated technologies and comparison with novel alternatives is a key feature which forms the basis for designing better bioseparation strategies that have potential for commercial scalability and can compete with traditional chemical production methods.

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Repulsive effect of salt on solvent extraction of 2,3-butanediol from aqueous fermentation solution

Cited by 24 publications

References 38 publications

Recent Progress in the Microbial Production of Pyruvic Acid

Recent Progress in the Microbial Production of Pyruvic Acid

Salting‐out extraction of bio‐based isobutanol from an aqueous solution

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

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