2024
DOI: 10.1021/acscatal.4c04145
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Repurposing Amide Bond-Forming Enzymes for Non-native Protein Modification

Tong Zhu,
Bian Wu

Abstract: Site-specific protein modification techniques have led to breakthroughs across diverse fields, such as cell imaging, proteomics, biomaterials, and pharmaceutical protein and peptide syntheses. Complementing the versatility of chemical approaches, enzymatic strategies offer high regioselectivity, facilitating precise functionalization within intricate biological matrices. This review highlights recent progress in the use of amide bond-forming enzymes, particularly those that have been repurposed to expand the r… Show more

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