Requalification of a Brazilian Trichoderma Collection and Screening of Its Capability to Decolourise Real Textile Effluent

Abstract

Water contamination with large amounts of industrial textile coloured effluents is an environmental concern. For the treatment of textile effluents, white-rot fungi have received extensive attention due to their powerful capability to produce oxidative (e.g., ligninolytic) enzymes. In addition, other groups of fungi, such as species of Aspergillus and Trichoderma, have also been used for textile effluents treatment. The main aim of the present study was to requalify a Brazilian Trichoderma culture collection of 51 Trichoderma strains, isolated from different sources in Brazil and preserved in the oldest Latin-American Fungal Service Culture Collection, The Micoteca URM WDCM 804 (Recife, Brazil). Fungal isolates were re-identified through a polyphasic approach including macro- and micro-morphology and molecular biology, and screened for their capability to decolourise real effluents collected directly from storage tanks of a textile manufacture. Trichoderma atroviride URM 4950 presented the best performance on the dye decolourisation in real textile effluent and can be considered in a scale-up process at industrial level. Overall, the potential of Trichoderma strains in decolourising real textile dye present in textile effluent and the production of the oxidative enzymes Lac, LiP and MnP was demonstrated. Fungal strains are available in the collection e-catalogue to be further explored from the biotechnological point of view.

Characterization of Trichoderma species from agricultural soils of Paraguay

“…The concatenation of ITS and tef1α sequences improved statistical support for species identification. Similar to our results, previous recent reports using the combination of ITS and tef1α have successfully characterized Trichoderma species (Lisboa et al, 2017;Haouhach et al, 2020).…”

Phylogenetic placement of Tritirachium strains from the URM culture collection originally founded by Augusto Chaves Batista (1916-1967) in Brazil, and the description of T. batistae sp. nov.

“…The URM culture collection is the most important fungal collection in Brazil, representing an abundant and diversified strain collection, which contains about 8,000 fungal names and have preserved 98,000 fungal cultures belonging to the most diverse groups. Among these cultures, there are strains deposited by Augusto Chaves Batista and his collaborators during their work in the former Instituto de Micologia da Universidade do Recife (IMUR), highlighting this collection as a reservoir of fungal diversity, which is recently being reviewed based on the modern taxonomic analyses (morphological and molecular), and is also being biotechnologically characterized in relation to the production of enzymes, and other secondary metabolites (Maciel et al 2013;Moreira et al 2014;Sousa et al 2015;Bezerra et al 2017a;b;Cruz et al 2017;Lisboa et al 2017;Barbosa et al 2018;Bezerra et al 2019).…”

Assessing the Fungal Simultaneous Removal Efficiency of Carbamazepine, Diclofenac and Ibuprofen in Aquatic Environment

“…Although the method mentioned above was especially described for Lac, in the present study, it was also extended for the detection of other enzymes (MnP and LiP) by using appropriate mediator substrates. The solid medium was supplemented with 25, 10, and 1 mL/L of VA (96%, Sigma-Aldrich, South Africa) for the LiP activity assay ( Tien and Kirk, 1988 ; Silva Lisboa et al, 2017 ). The VA solution of 20 mM was prepared in 0.2 M sodium tartrate buffer (pH 3.0).…”

“…The LiP activity was determined by monitoring the oxidation of 20 mM VA in 0.2 M sodium tartrate buffer (pH 3.0) with 2 mM hydrogen peroxide at 310 nm. The extinction coefficient considered was ε310 nm = 9.3 × 10 3 M –1 cm –1 to monitor the increase in substrate absorbance ( Tien and Kirk, 1988 ; Silva Lisboa et al, 2017 ). The MnP activity was determined by monitoring the oxidation of 20 mM 2,6-DMP in 0.5 M sodium tartrate buffer (pH 4.5) and 20 mM manganese sulfate with 2 mM hydrogen peroxide at 470 nm.…”