Requirement for Different Presenting Cells and for Different Processing Mechanisms by Human CD4 T Helper Clones Specific for M. tuberculosis Antigens

Manca, Fabrizio; Valle, Maurizio; Megiovanni, Annamaria; Pira, Giuseppina Li; Fenoglio, Daniela; Kunkl, Annalisa; Merlo, Andrea; Terranova, Paola; Bottone, Laura; Balbi, Bruno; Lantero, Sabina; Rossi, Giovanni A.

doi:10.1016/s0198-8859(98)00025-1

Cited by 2 publications

(3 citation statements)

References 34 publications

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“…T cell lines allow a careful characterization of the fine specificity and of the clonal heterogeneity of specific CD4 cells. We have already used PPD‐specific lines and clones from peripheral blood and from pleural exudates [18] to study APC requirements and processing requirements [27].…”

Section: Discussionmentioning

confidence: 99%

“…T cell lines allow a careful characterization of the fine specificity and of the clonal heterogeneity of specific CD4 cells. We have already used PPDspecific lines and clones from peripheral blood and from pleural exudates [18] to study APC requirements and processing requirements [27]. Peptides 0 2 4 6 8 1 0 1 2 1 2 3 4 5 6 7 8 9 10 11 12 15 16 17 18 19 20 21 23 24 25 26 27 28 29 30 31 Ag85ABC T cell lines 0 2 4 6 8 1 0 1 2 1 2 3 4 5 6 7 8 9 10 11 12 15 16 17 18 19 20 21 23 24 25 26 27 28 29 30 31 14 Response to deleted PEP11 peptides by PBMC and by T cell lines T cell lines 0 1 2 3 4 5 6 Ag85ABC T cell lines The data we present show that within the population of PPDresponsive lymphocytes, the majority is specific for Ag85.…”

Section: Discussionmentioning

confidence: 99%

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Epitope focus, clonal composition and Th1 phenotype of the human CD4 response to the secretory mycobacterial antigen Ag85

Valle

Megiovanni

Merlo

et al. 2001

Clinical and Experimental Immunology

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SUMMARYLymphoproliferation of healthy donors was tested against mycobacterial antigens (PPD, Ag85, Ag85 peptides). All PPD responders recognized the secretory antigen Ag85 and the peptide specificity for Ag85B was defined. Peptide 91±108 was recognized by 85% of donors. In addition, all CD4 T cell lines generated from 12 donors against PPD or Ag85 responded to 91±108. When this peptide was used to generate T cell lines, the cells responded also to tuberculins from atypical mycobacterial species. Thus the cross-reactive peptide behaved as quasi-universal. The analysis of TCR-BV gene usage by cell lines showed that most Ag85-specific T cells correspond to 91±108-specific clonotypes. Intracytoplasmic staining of cell lines after phorbol myristate acetate stimulation resulted in dominance of interferongamma (IFN-g)-IL-4 double-positive cells, whereas antigen stimulation resulted in production of IFN-g only. The data show that peptide 91±108 is the major focus of the CD4 response to mycobacterial antigens in peripheral blood mononuclear cells and in T cell lines from PPD responders.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epitope focus, clonal composition and Th1 phenotype of the human CD4 response to the secretory mycobacterial antigen Ag85

Valle

Megiovanni

Merlo

et al. 2001

Clinical and Experimental Immunology

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“…, manuscript submitted), as well as antigens of M. tuberculosis 61 which were shown to be presented to CD4 T cells independent of newly synthesized MHC class II molecules or endosomal acidification. Furthermore, presentation of unidentified M. tuberculosis antigens to CD4 T‐cell clones was demonstrated to be independent of cysteine and aspartic proteinases for the majority of T‐cell clones 92 suggesting a bypass of lysosomal processing for many epitopes from viable M. tuberculosis. 6,93 Presentation by recycling MHC class II molecules was recently reported to be characteristic for the C‐terminal fragment of MSP‐1 protein of the mouse malaria parasite Plasmodium chabaudi chabaudi 46 suggesting that early endosomes are also used for processing of protozoan proteins for presentation to CD4 T cells.…”

Section: How Might Diversity In Antigen Presentation Influence the Immentioning

confidence: 99%

Diversity in MHC class II antigen presentation

Robinson

Delvig

2002

Immunology

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Summary Processing exogenous and endogenous proteins for presentation by major histocompatibility complex (MHC) molecules to T cells is the defining function of antigen‐presenting cells (APC) as major regulatory cells in the acquired immune response. MHC class II‐restricted antigen presentation to CD4 T cells is achieved by an essentially common pathway that is subject to variation with regard to the location and extent of degradation of protein antigens and the site of peptide binding to MHC class II molecules. These subtle variations reveal a surprising flexibility in the ways a diverse peptide repertoire is displayed on the APC surface. This diversity may have profound consequences for the induction of immunity to infection and tumours, as well as autoimmunity and tolerance.

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Requirement for Different Presenting Cells and for Different Processing Mechanisms by Human CD4 T Helper Clones Specific for M. tuberculosis Antigens

Cited by 2 publications

References 34 publications

Epitope focus, clonal composition and Th1 phenotype of the human CD4 response to the secretory mycobacterial antigen Ag85

Epitope focus, clonal composition and Th1 phenotype of the human CD4 response to the secretory mycobacterial antigen Ag85

Diversity in MHC class II antigen presentation

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