Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus

Jiang, Cheng; Iu, Betty; Singh, Jas

doi:10.1007/bf00049344

Cited by 265 publications

(165 citation statements)

References 14 publications

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“…Among genes up-regulated by 30 min of EL, we found a number of known light-regulatory elements, including the Gbox (CACGTG) (24), and a GCCAC motif previously identified as overrepresented in light-induced promoters (SORLIPs) (25). We also found an ACGT motif (26), a CCGAC motif (27), and an ATCCAAT motif (28) (Fig. S1F).…”

Section: El Triggers Redox Stress and Changes The Expression Of Hundrmentioning

confidence: 74%

Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

Jung

Crisp

Estavillo

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

136

124

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Sunlight provides energy for photosynthesis and is essential for nearly all life on earth. However, too much or too little light or rapidly fluctuating light conditions cause stress to plants. Rapid changes in the amount of light are perceived as a change in the reduced/oxidized (redox) state of photosynthetic electron transport components in chloroplasts. However, how this generates a signal that is relayed to changes in nuclear gene expression is not well understood. We modified redox state in the reference plant, Arabidopsis thaliana, using either excess light or low light plus the herbicide DBMIB (2,5-dibromo-3-methyl-6-isopropyl-pbenzoquinone), a well-known inhibitor of photosynthetic electron transport. Modification of redox state caused a change in expression of a common set of about 750 genes, many of which are known stress-responsive genes. Among the most highly enriched promoter elements in the induced gene set were heat-shock elements (HSEs), known motifs that change gene expression in response to high temperature in many systems. We show that HSEs from the promoter of the ASCORBATE PEROXIDASE 2 (APX2) gene were necessary and sufficient for APX2 expression in conditions of excess light, or under low light plus the herbicide. We tested APX2 expression phenotypes in overexpression and loss-of-function mutants of 15 Arabidopsis A-type heat-shock transcription factors (HSFs), and identified HSFA1D, HSFA2, and HSFA3 as key factors regulating APX2 expression in diverse stress conditions. Excess light regulates both the subcellular location of HSFA1D and its biochemical properties, making it a key early component of the excess light stress network of plants.

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Section: El Triggers Redox Stress and Changes The Expression Of Hundrmentioning

confidence: 74%

Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

Jung

Crisp

Estavillo

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

136

124

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“…Analysis of the AtDGK2 promoter for the presence of potential cis regulatory elements using the PLACE (available on the World Wide Web at www.dna.affrc.go.jp/htdocs/PLACE/ fasta.html) and PlantCARE (available on the World Wide Web at intra.psb.ugent.be:8080/PlantCARE) interfaces revealed cis elements that may contribute to cold-responsive gene expression (Fig. 9a) (43)(44)(45)(46). We tested the transcriptional activation of AtDGK2 by subjecting promAtDGK2::GUS reporter lines to cold stress.…”

Section: Fig 2 Phylogenetic Analysis Of Plant Dgk Isozymesmentioning

confidence: 99%

AtDGK2, a Novel Diacylglycerol Kinase from Arabidopsis thaliana, Phosphorylates 1-Stearoyl-2-arachidonoyl-sn-glycerol and 1,2-Dioleoyl-sn-glycerol and Exhibits Cold-inducible Gene Expression

Gómez-Meriño

Brearley

Ornatowska

et al. 2004

Journal of Biological Chemistry

117

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“…An important advance in this regard was the recent identification of the C-repeat͞dehydration responsive element (DRE) cis-acting DNA regulatory element (6)(7)(8). The element, which has a 5-bp core sequence of CCGAC, designated the C-repeat (7), is present in one to multiple copies in the promoters of many cold-regulated plant genes, including the Arabidopsis genes COR15a (7) and COR78͞RD29A (ref.…”

mentioning

confidence: 99%

“…The element, which has a 5-bp core sequence of CCGAC, designated the C-repeat (7), is present in one to multiple copies in the promoters of many cold-regulated plant genes, including the Arabidopsis genes COR15a (7) and COR78͞RD29A (ref. 6; COR78 and RD29A are alternative designations for the same gene) and the Brassica napus gene BN115 (8). Deletion analysis of the Arabidopsis COR15a promoter indicated that the C-repeat might be part of a cis-acting cold-regulatory DNA element (7).…”

mentioning

confidence: 99%

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Stockinger

Gilmour

Thomashow

1997

Proc. Natl. Acad. Sci. U.S.A.

1,588

1,203

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Recent efforts have defined a cis-acting DNA regulatory element in plants, the C-repeat͞dehydration responsive element (DRE), that stimulates transcription in response to low temperature and water deficit. Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat͞DRE binding factor, CBF1 (C-repeat͞DRE Binding Factor 1). Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain. CBF1 also has an AP2 domain, which is a DNA-binding motif of about 60 aa present in the Arabidopsis proteins APETALA2, AINTEGUMENTA, and TINY; the tobacco ethylene response element binding proteins; and numerous other plant proteins of unknown function. The transcript levels for CBF1, which appears to be a single or low copy number gene, did not change appreciably in plants exposed to low temperature or in detached leaves subjected to water deficit. Binding of CBF1 to the C-repeat͞DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli. Moreover, expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat͞DRE as an upstream activator sequence but not mutant versions of the DNA element. We conclude that CBF1 can function as a transcriptional activator that binds to the C-repeat͞DRE DNA regulatory element and, thus, is likely to have a role in cold-and dehydrationregulated gene expression in Arabidopsis.

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Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus

Cited by 265 publications

References 14 publications

Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

AtDGK2, a Novel Diacylglycerol Kinase from Arabidopsis thaliana, Phosphorylates 1-Stearoyl-2-arachidonoyl-sn-glycerol and 1,2-Dioleoyl-sn-glycerol and Exhibits Cold-inducible Gene Expression

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

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