Requirement of Mis6 Centromere Connector for Localizing a CENP-A-Like Protein in Fission Yeast

Takahashi, Kohske; Chen, Ee Sin; Yanagida, Mitsuhiro

doi:10.1126/science.288.5474.2215

Cited by 368 publications

(467 citation statements)

References 18 publications

Supporting

Mentioning

438

Contrasting

Unclassified

Order By: Relevance

“…In S. cerevisiae, centromere localization of meiotic cohesin Rec8 is reduced by loss of CHL4 (Marston et al, 2004), which is a homolog of S. pombe Mis15, and Mis15 requires Mis6 for its centromere localization (Hayashi et al, 2004). Mis6 is also required for loading of Cnp1, a CENP-A homolog (Takahashi et al, 2000). Thus, the Mis6 complex forms a "foothold" for the Rec8-mediated mono-orientation kinetochore, most likely through interactions with CENP-Aand CENP-C-associated regions of the centromere ( Figure 10A).…”

Section: Mis6 Complex: Basic Architecture Of the Kinetochorementioning

confidence: 99%

“…Recently 13 proteins were purified as a Mis6-containing complex by biochemical purification . These proteins include Sim4 and Mis15, which have been reported to depend on the Mis6 protein for their centromere localization (Takahashi et al, 2000;Pidoux et al, 2003;Hayashi et al, 2004). Thus, it is likely that these proteins compose the Mis6 complex (also called the Sim4 complex in Liu et al, 2005), which corresponds to the S. cerevisiae COMA and Ctf19 complexes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Hayashi

Asakawa

Haraguchi

et al. 2006

MBoC

View full text Add to dashboard Cite

During the transition from mitosis to meiosis, the kinetochore undergoes significant reorganization, switching from a bipolar to a monopolar orientation. To examine the centromere proteins that are involved in fundamental reorganization in meiosis, we observed the localization of 22 mitotic and 2 meiotic protein components of the kinetochore during meiosis in living cells of the fission yeast. We found that the 22 mitotic proteins can be classified into three groups: the Mis6-like group, the NMS (Ndc80-Mis12-Spc7) group, and the DASH group, based on their meiotic behavior. Mis6-like group proteins remain at the centromere throughout meiosis. NMS group proteins disappear from the centromere at the onset of meiosis and reappear at the centromere in two steps in late prophase. DASH group proteins appear shortly before metaphase of meiosis I. These observations suggest that Mis6-like group proteins constitute the structural basis of the centromere and that the NMS and DASH group proteins reassemble to establish the functional metaphase kinetochore. On the other hand, the meiosis-specific protein Moa1, which plays an important role in forming the meiotic monopolar kinetochore, is loaded onto the centromere significantly earlier than the NMS group, whereas another meiosis-specific protein, Sgo1, is loaded at times similar to the NMS group.

show abstract

Section: Mis6 Complex: Basic Architecture Of the Kinetochorementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Hayashi

Asakawa

Haraguchi

et al. 2006

MBoC

View full text Add to dashboard Cite

show abstract

“…One hallmark of all kinetochores is the essential CenH3 (Palmer et al, 1987;Stoler et al, 1995;Buchwitz et al, 1999;Henikoff et al, 2000;Takahashi et al, 2000;Sanyal and Carbon, 2002;Talbert et al, 2002;Zhong et al, 2002;Edwards and Murray, 2005). CenH3s contain a unique N terminus and a well-conserved C terminus that is highly homologous to histone H3 (Malik and Henikoff, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Outer kinetochore complexes include the conserved NDC80 (Ndc80, Spc24, Spc25, and Nuf2) and DAM1 (Dam1, Ask1, Duo1, Dad1, Dad2, Dad3, Dad4, Spc19, Spc34, and Hsk3) complexes. DAM1 is considered to be the outermost complex because it requires microtubules and all other complexes for kinetochore localization (Enquist-Newman et al, 2001;Janke et al, 2002;Li et al, 2002).One hallmark of all kinetochores is the essential CenH3 (Palmer et al, 1987;Stoler et al, 1995;Buchwitz et al, 1999;Henikoff et al, 2000;Takahashi et al, 2000;Sanyal and Carbon, 2002;Talbert et al, 2002;Zhong et al, 2002;Edwards and Murray, 2005). CenH3s contain a unique N terminus and a well-conserved C terminus that is highly homologous to histone H3 (Malik and Henikoff, 2003).…”

mentioning

confidence: 99%

De Novo Kinetochore Assembly Requires the Centromeric Histone H3 Variant

Collins

Castillo

Tatsutani

et al. 2005

MBoC

View full text Add to dashboard Cite

Kinetochores mediate chromosome attachment to the mitotic spindle to ensure accurate chromosome segregation. Budding yeast is an excellent organism for kinetochore assembly studies because it has a simple defined centromere sequence responsible for the localization of >65 proteins. In addition, yeast is the only organism where a conditional centromere is available to allow studies of de novo kinetochore assembly. Using a conditional centromere, we found that yeast kinetochore assembly is not temporally restricted and can occur in both G 1 phase and prometaphase. We performed the first investigation of kinetochore assembly in the absence of the centromeric histone H3 variant Cse4 and found that all proteins tested depend on Cse4 to localize. Consistent with this observation, Cse4-depleted cells had severe chromosome segregation defects. We therefore propose that yeast kinetochore assembly requires both centromeric DNA specificity and centromeric chromatin. INTRODUCTIONAccurate chromosome segregation in mitosis and meiosis is essential for the maintenance of genomic stability. Chromosomes attach to the mitotic spindle at the kinetochore, the protein complex that assembles onto centromeric DNA. Although kinetochore function is conserved, the underlying centromeric DNA is highly variable. Budding yeast contain a 125-base pair sequence-specific centromere that is sufficient for kinetochore formation (Fitzgerald-Hayes et al., 1982). In contrast, centromeres in multicellular eukaryotes are composed of megabases of highly repetitive DNA that lack sequence specificity (for review, see Sullivan et al., 2001). In these organisms, kinetochore assembly seems to be propagated by unidentified epigenetic component(s) (Karpen and Allshire, 1997;Sullivan et al., 2001).The best-characterized kinetochore is in budding yeast where Ͼ65 components have been identified that constitutively localize to the kinetochore (for reviews, see Biggins and Walczak, 2003;McAinsh et al., 2003). Most of the yeast kinetochore proteins are found in biochemically distinct complexes known as the CBF3, CTF19/COMA, MTW1, NDC80, and DAM1 complexes that seem to assemble on a single centromeric nucleosome (Meluh et al., 1998). Although the exact architecture of the kinetochore is not known, dependency relationships subdivide the kinetochore into inner, central, and outer domains. The inner kinetochore contains the CBF3 complex (Ndc10, Cep3, Skp1, and Ctf13) as well as the DNA binding proteins Mif2, Cbf1, and the yeast centromeric histone H3 variant (CenH3) Cse4. CBF3 binds directly to the centromeric DNA and is thought to nucleate kinetochore assembly because all kinetochore proteins require it for localization (Russell et al., 1999;Goshima and Yanagida, 2000;He et al., 2001;Janke et al., 2001Janke et al., , 2002. The central kinetochore contains the MTW1 (Mtw1, Dsn1, Nnf1, and Nsl1) and CTF19/COMA (Ctf19, Mcm16, Mcm19, Mcm21, Mcm22, Ctf3, Chl4, Okp1, Ame1, Iml3, Nkp1, and Nkp2) complexes. CTF19/COMA can be further divided into two subcomplexes, with Ame1...

show abstract

“…This indicates that functional CENP-A nucleosomes are assembled into chromatin as a consequence of CENP-C or CENP-I ectopic localization . Indeed, the individual CENPs that comprise the recently identified CENP-H/I/K/M complex have been implicated in CENP-A assembly in chicken and human cells (Takahashi et al 2000;Okada et al 2006).…”

Section: The Centromerementioning

confidence: 99%

The Centromere: Epigenetic Control of Chromosome Segregation during Mitosis

Westhorpe

Straight

2014

Cold Spring Harb Perspect Biol

149

View full text Add to dashboard Cite

A fundamental challenge for the survival of all organisms is maintaining the integrity of the genome in all cells. Cells must therefore segregate their replicated genome equally during each cell division. Eukaryotic organisms package their genome into a number of physically distinct chromosomes, which replicate during S phase and condense during prophase of mitosis to form paired sister chromatids. During mitosis, cells form a physical connection between each sister chromatid and microtubules of the mitotic spindle, which segregate one copy of each chromatid to each new daughter cell. The centromere is the DNA locus on each chromosome that creates the site of this connection. In this review, we present a brief history of centromere research and discuss our current knowledge of centromere establishment, maintenance, composition, structure, and function in mitosis.

show abstract

Requirement of Mis6 Centromere Connector for Localizing a CENP-A-Like Protein in Fission Yeast

Cited by 368 publications

References 18 publications

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

De Novo Kinetochore Assembly Requires the Centromeric Histone H3 Variant

The Centromere: Epigenetic Control of Chromosome Segregation during Mitosis

Contact Info

Product

Resources

About