1994
DOI: 10.1093/nar/22.24.5360
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Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA

Abstract: Vaccinia DNA topoisomerase binds duplex DNA and forms a covalent adduct at sites containing a conserved sequence element 5'(C/T)CCTT1 in the scissile strand.

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Cited by 35 publications
(41 citation statements)
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“…Enhancement of permanganate sensitivity at ϩ1T was not detected when the experiments were performed with equivalent amounts of Topo-(Phe-274), a mutated version of the vaccinia enzyme in which the active site tyrosine is substituted by phenylalanine (not shown). Because Topo(Phe-274) binds to the CCCTT recognition site but does not transesterify (Shuman, 1991b;Sekiguchi and Shuman, 1994b), we surmise that induction of permanganate sensitivity requires strand cleavage by the topoisomerase.…”
Section: Resultsmentioning
confidence: 80%
See 1 more Smart Citation
“…Enhancement of permanganate sensitivity at ϩ1T was not detected when the experiments were performed with equivalent amounts of Topo-(Phe-274), a mutated version of the vaccinia enzyme in which the active site tyrosine is substituted by phenylalanine (not shown). Because Topo(Phe-274) binds to the CCCTT recognition site but does not transesterify (Shuman, 1991b;Sekiguchi and Shuman, 1994b), we surmise that induction of permanganate sensitivity requires strand cleavage by the topoisomerase.…”
Section: Resultsmentioning
confidence: 80%
“…For example, there is evidence that vaccinia topoisomerase makes contacts with the DNA downstream of the cleavage site. Increasing the length of the duplex region downstream of the scissile bond incrementally from 6 to 10 to 12 bp strongly enhances noncovalent binding (Sekiguchi and Shuman, 1994b). This suggests that the topoisomerase interacts with the DNA duplex at a distance from the CCCTT element of about one helical turn.…”
mentioning
confidence: 99%
“…One explanation is that the specificity of vaccinia TopIB for transesterification at the pentapyrimidine sequence 5Ј-(T/ C)CCTT2 is enforced by a requirement for target site recognition prior to assembly of the active site. This requirement is sensible given that noncovalent binding of vaccinia TopIB to duplex DNA is relatively nonspecific (63). Site recognition to trigger transesterification entails contacts to specific DNA bases and backbone phosphate oxygens that flank the scissile phosphodiester (31,32).…”
Section: Resultsmentioning
confidence: 99%
“…A native gel mobility shift assay (6,21) was used to analyze the DNA binding properties of the R223 mutant proteins. The 32 P-labeled DNA ligand was a 24-bp CCCTTcontaining duplex (Fig.…”
Section: Mutagenesis Strategy and Production Of Mutant Proteins-mentioning
confidence: 99%