Resazurin Microtiter Assay for Clarithromycin Susceptibility Testing of Clinical Isolates of <i>Mycobacterium abscessus</i> Group

Carvalho, Natália Fernandes Garcia de; Sato, Daisy Nakamura; Pavan, Fernando Rogério; Ferrazoli, Lucilaine; Chimara, Érica

doi:10.1002/jcla.21933

Cited by 15 publications

(6 citation statements)

References 26 publications

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“…Meanwhile, similar overall trends were observed for AZM resistance (see Table S2 in the supplemental material). Notably, the acquired CLA resistance rate of 8.1% (31/381) was lower than those reported in other studies in China (33.95%) (12) and South Korea (15.84%) (13), higher than those reported in Brazil (5.55%) (14) and the United States (2.51%), and similar to the rate in France (9.09%) (15). These variations may be the results of differences in isolates or in patient treatment histories among the various studies.…”

contrasting

confidence: 60%

Genetic Correlation of Antibiotic Susceptibility and Resistance Genotyping for the Mycobacterium abscessus Group

Liu

Song

et al. 2019

Antimicrob Agents Chemother

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contrasting

confidence: 60%

Genetic Correlation of Antibiotic Susceptibility and Resistance Genotyping for the Mycobacterium abscessus Group

Liu

Song

et al. 2019

Antimicrob Agents Chemother

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“…The proportion of M. abscessus isolates that acquired resistance to CLA (ERT assessment) was relatively high (33.95%, 55/162) compared to other countries: Japan, 12.79% (11/86); Korea, 15.84% (64/404); UK, 6.54% (35/535); France, 9.09% (15/165); US, 2.51% (9/358); and Brazil, 5.55% (2/36). The countries included in this comparison consisted of those where M. abscessus infections were most frequently studied and, consequently, were the largest sources of available data over the last 5 years (Harada et al, 2012 ; Lee et al, 2014 ; Brown-Elliott et al, 2015 ; Nie et al, 2015 ; Pang et al, 2015 ; Rubio et al, 2015 ; Cowman et al, 2016 ; Garcia de Carvalho et al, 2016 ; Mougari et al, 2016 ). Undoubtedly, uneven economic development, and differences in the production, management and clinical use of antibiotics contribute to this disparity in rates of CLA resistance observed among countries.…”

Section: Discussionmentioning

confidence: 99%

“…Recent articles have only explored the relationship between susceptibility and subtype (Pang et al, 2015 ), or just mentioned that the erm(41) genotype was related to clarithromycin resistance after 14 days' incubation, without detailed genetic information or susceptibility test results (Nie et al, 2015 ). Moreover, the genotype and antibiotic susceptibility of M. abscessus may vary worldwide (Harada et al, 2012 ; Lee et al, 2014 ; Brown-Elliott et al, 2015 ; Nie et al, 2015 ; Pang et al, 2015 ; Rubio et al, 2015 ; Cowman et al, 2016 ; Garcia de Carvalho et al, 2016 ; Mougari et al, 2016 ). The study described herein was undertaken to establish the relationship between antibiotic susceptibility and M. abscessus genotype in China, and to compare it to that found in other countries.…”

Section: Introductionmentioning

confidence: 99%

Relationship between Antibiotic Susceptibility and Genotype in Mycobacterium abscessus Clinical Isolates

Yang

Chu

et al. 2017

Front. Microbiol.

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This study aimed to determine the antibiotic susceptibility and resistance related genotypes of Mycobacterium abscessus. One hundred sixty-two clinical isolates were collected. Genomic data were obtained by whole genome sequencing. Single nucleotide polymorphism (SNP) analysis was conducted using the NCBI GenBank database and BLAST algorithm. The following genes were of interest: erm(41), rrl and rrs. Erm(41) was further divided into 3 sequevars: erm(41)C28, erm(41)T28, and M type [erm(41) with deletions in nucleotides 64 and 65, or 159 through 432]. Antibiotic susceptibility was assessed at 3 days (early reading time, ERT) and 14 days (late reading time, LRT) after clarithromycin (CLA) treatment. Three patterns of CLA resistance were observed. (1) Fifty-five (acquired resistance) isolates [45 erm(41)T28, 1 erm(41)C28 and 9 M type] exhibited MIC ≥8 mg/L at ERT; among these isolates, 10 had an rrl 2058/2059 mutation. (2) Sixty-two subsp. abscessus and 2 subsp. massiliense (induced resistance) isolates exhibited MIC ≤4 mg/L at ERT, but ≥8 mg/L at LRT. (3) Forty-three (sensitive and intermediate) isolates [14 erm(41)C28, 1 erm(41)T28, and 28 M type] exhibited MIC ≤4 mg/L at both ERT and LRT. No rrs 1408 mutation or other meaningful SNP was found in 3 amikacin-resistant isolates. No correlation was found between rrl, erm(41) or rrs and susceptibility to the 8 other antibiotics tested. The rrl and erm(41) genotypes could predict the CLA resistance of M. abscessus clinical isolates. China has a large number of CLA-resistant M. abscessus isolates with erm(41)T28 sequevar. Treatment of M. abscessus infections should be based upon a comprehensive consideration of factors that include genotype and geographic location.

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“…The MBC was determined after 5–7 days incubation at 37°C. The MBC was the lowest extract concentration that prevented 99.9% bacterial growth expressed in CFU/mL [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense

Viana¹,

Zagmignan²,

Lobato³

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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The incidence of infections caused by rapidly growing mycobacteria (RGM), especially Mycobacterium abscessus subsp. massiliense (Mabs), is increasing worldwide. Severe infections are associated with abscess formation and strong inflammatory response. This study evaluated the antimicrobial and anti-inflammatory activities of a hydroalcoholic extract (BoHE) and ethyl acetate fraction (BoEA) of Bixa orellana leaves. Antimicrobial activity was evaluated by broth microdilution to determine the minimum inhibitory (MIC) and the minimum bactericidal (MBC) concentrations. Cytotoxicity was evaluated using erythrocytes and RAW 264.7 cells. Nitric oxide (NO) was assayed in stimulated RAW 264.7 cells, and inflammatory cell migration and acute toxicity were evaluated in a Mabs-induced peritonitis mouse model. The compounds present in BoEA were identified by high performance liquid chromatography and mass spectrometry (HPLC-MS). The MIC and MBC values were 2.34 mg/mL and 37.5 mg/mL for BoHE and 0.39 mg/mL and 6.25 mg/mL for BoEA. The extracts did not induce significant toxicity in erythrocytes and RAW 264.7 cells. High levels of NO induced by Mabs were decreased by treatment with both extracts. The anti-inflammatory activity was confirmed in vivo by significant reduction of the cell migration to the peritoneum following BoHE and BoEA pretreatment. Animals treated with BoHE or BoEA did not show signs of acute toxicity in stomach, liver, and kidney. The chemical characterization of BoEA (the most active extract) revealed that kaempferol-3-O-coumaroyl glucose is its major component. The extract of B. orellana may be effective for treating infections caused by Mabs.

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Resazurin Microtiter Assay for Clarithromycin Susceptibility Testing of Clinical Isolates of Mycobacterium abscessus Group

Abstract: The present study showed 100% concordance between both readings, with REMA providing easier to read and report results benefit. This change in reading can also reflect on the MIC determination and report, improving the test.

Cited by 15 publications

References 26 publications

Genetic Correlation of Antibiotic Susceptibility and Resistance Genotyping for the Mycobacterium abscessus Group

Genetic Correlation of Antibiotic Susceptibility and Resistance Genotyping for the Mycobacterium abscessus Group

Relationship between Antibiotic Susceptibility and Genotype in Mycobacterium abscessus Clinical Isolates

Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense

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