Rescue of Fructose-Induced Metabolic Syndrome by Antibiotics or Faecal Transplantation in a Rat Model of Obesity

Abstract

A fructose-rich diet can induce metabolic syndrome, a combination of health disorders that increases the risk of diabetes and cardiovascular diseases. Diet is also known to alter the microbial composition of the gut, although it is not clear whether such alteration contributes to the development of metabolic syndrome. The aim of this work was to assess the possible link between the gut microbiota and the development of diet-induced metabolic syndrome in a rat model of obesity. Rats were fed either a standard or high-fructose diet. Groups of fructose-fed rats were treated with either antibiotics or faecal samples from control rats by oral gavage. Body composition, plasma metabolic parameters and markers of tissue oxidative stress were measured in all groups. A 16S DNA-sequencing approach was used to evaluate the bacterial composition of the gut of animals under different diets. The fructose-rich diet induced markers of metabolic syndrome, inflammation and oxidative stress, that were all significantly reduced when the animals were treated with antibiotic or faecal samples. The number of members of two bacterial genera, Coprococcus and Ruminococcus, was increased by the fructose-rich diet and reduced by both antibiotic and faecal treatments, pointing to a correlation between their abundance and the development of the metabolic syndrome. Our data indicate that in rats fed a fructose-rich diet the development of metabolic syndrome is directly correlated with variations of the gut content of specific bacterial taxa.

Major microbiota dysbiosis in severe obesity: fate after bariatric surgery

“…Rodent data indicate that Coprococcus increases in fructose-fed animals, which develop metabolic syndrome. Coprococcus decreases in animals after either antibiotic treatment or faecal transfer, which improved their metabolic phenotype 44. In contrast, another MGS cluster was composed of species strongly reduced with increasing trunk-fat.…”

Beneficial Effect of Bis(Hinokitiolato)Zn Complex on High-fat Diet-induced Lipid Accumulation in Mouse Liver and Kidney

“…The experimental animals were fed an HFD or highfructose diet to establish the metabolic syndrome or DM model (20,21). Therefore, this study examined the histopathology of the livers and kidneys of C57BL/6J mice 1145…”

“…Furthermore, only a few studies such as that of Moroki et al (19) have reported experiments showing the effects of Zn complexes on lipid metabolism. Thus, the effects of Zn compounds on lipid metabolism have not been sufficiently examined yet.The experimental animals were fed an HFD or highfructose diet to establish the metabolic syndrome or DM model (20,21). Therefore, this study examined the histopathology of the livers and kidneys of C57BL/6J mice 1145…”

Nonalcoholic fatty pancreas disease as an endogenous alcoholic fatty pancreas disease

“…This amounts to an ethanol elimination rate of 528 g per day, without including gastric, colonic, and hepatic FPM. This key role of gut microbiota in FPM of ethanol is supported by studies showing that oral antibiotics protect against both NAFLD/NAFPD [130][131][132][133][134] and alcoholic liver disease [135]. Furthermore, germ-free animals are also protected against dietinduced obesity-related disorders [136][137][138] as well as ethanol-induced liver injury [139].…”