Rescue of human corneal epithelial cells after alkaline insult using renalase derived peptide, RP-220

Potts, Luke B.; Phillips, Casie; Hwang, Munok; Fulcher, Samuel; Choi, Hosoon

doi:10.18240/ijo.2019.11.01

Cited by 6 publications

(9 citation statements)

References 33 publications

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“…The RP-220 peptide sequence and related peptides common to all known forms of RNLS have been used in most of our studies. The RP-220 site (RNLS1 aa 220-239) has no known enzymatic activities, but it has demonstrated a prosurvival effect after acute injury to kidney, heart, and corneal epithelial cells [12,51,52]. In select systems, the signals induced by full-length RNLS have been replicated using RP-220 [12,50,52].…”

Section: Rnls Stimulates Distinct Intracellular Signalsmentioning

confidence: 99%

“…The RP-220 site (RNLS1 aa 220-239) has no known enzymatic activities, but it has demonstrated a prosurvival effect after acute injury to kidney, heart, and corneal epithelial cells [12,51,52]. In select systems, the signals induced by full-length RNLS have been replicated using RP-220 [12,50,52]. The retained functionality when oxidase-and FADindependent activities are absent provides compelling evidence of the signaling function of this RNLS site.…”

Section: Rnls Stimulates Distinct Intracellular Signalsmentioning

confidence: 99%

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Renalase: A Multi-Functional Signaling Molecule with Roles in Gastrointestinal Disease

Pointer

Gorelick

Desir

2021

Cells

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The survival factor renalase (RNLS) is a recently discovered secretory protein with potent prosurvival and anti-inflammatory effects. Several evolutionarily conserved RNLS domains are critical to its function. These include a 20 aa site that encodes for its prosurvival effects. Its prosurvival effects are shown in GI disease models including acute cerulein pancreatitis. In rodent models of pancreatic cancer and human cancer tissues, increased RNLS expression promotes cancer cell survival but shortens life expectancy. This 37 kD protein can regulate cell signaling as an extracellular molecule and probably also at intracellular sites. Extracellular RNLS signals through a specific plasma membrane calcium export transporter; this interaction appears most relevant to acute injury and cancer. Preliminary studies using RNLS agonists and antagonists, as well as various preclinical disease models, suggest that the immunologic and prosurvival effects of RNLS will be relevant to diverse pathologies that include acute organ injuries and select cancers. Future studies should define the roles of RNLS in intestinal diseases, characterizing the RNLS-activated pathways linked to cell survival and developing therapeutic agents that can increase or decrease RNLS in relevant clinical settings.

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Section: Rnls Stimulates Distinct Intracellular Signalsmentioning

confidence: 99%

Section: Rnls Stimulates Distinct Intracellular Signalsmentioning

confidence: 99%

Renalase: A Multi-Functional Signaling Molecule with Roles in Gastrointestinal Disease

Pointer

Gorelick

Desir

2021

Cells

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“…Taken together, mounting research ( 11 , 15 , 17 , 18 , 44 , 45 , 47 , 48 , 68 , 84 , 102 , 105 , 117 ) adds substantial evidence that renalase confers cytoprotection, by exerting anti-inflammatory, antioxidant, and anti-apoptotic effects, providing a hypothesis of a single molecule for targeting various pathways. Given that tissue safety is impeded by the silencing of the renalase gene, recombinant renalase administration, accordingly, generates robust protection, and preserves the phenotypes of injured cells, including cardiomyocytes, and upholds the possibility that renalase administration may be relevant in cardiac fibroproliferation.…”

Section: The Pathophysiological Relevance Of Renalase As An Anti-infl...mentioning

confidence: 99%

“…Additional studies provided evidence that renalase’s distribution is spread far beyond the kidneys and heart. Therefore, its tissue expression pattern, to varying extents, is revealed in the liver ( 19 , 44 ), small intestines ( 45 ), skeletal muscles ( 46 ), pancreas ( 22 , 47 ), cornea ( 48 ), brain and peripheral nerves ( 49 , 50 ), and malignant tissue as well ( 22 , 23 , 51 ), implying renalase’s remote role in general homeostasis and its possible relevant biologic activity. Accordingly, the latest research indicates a potential role for renalase in placental development and function, owing to its expression in the placenta throughout human gestation ( 25 ).…”

Section: Renalase’s Structure Expression and Cell Biologymentioning

confidence: 99%

“…Such results set the stage for renalase’s pathophysiology to be moved to the next round of research, far beyond its catalytic activities. Indeed, in recent years, renalase has been established as a molecule exerting anti-inflammatory and antiapoptotic actions, with the intention of cell survival, and that significantly protects organs and tissues, independently of its enzymatic traits ( 10 – 25 , 44 – 48 ). Surprisingly, tumor cells use renalase’s action as a survival strategy ( 10 , 11 , 51 , 67 ).…”

Section: Renalase’s Structure Expression and Cell Biologymentioning

confidence: 99%

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The Scientific Rationale for the Introduction of Renalase in the Concept of Cardiac Fibrosis

Stojanović

Mitić

Stojanović

et al. 2022

Front. Cardiovasc. Med.

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Cardiac fibrosis represents a redundant accumulation of extracellular matrix proteins, resulting from a cascade of pathophysiological events involved in an ineffective healing response, that eventually leads to heart failure. The pathophysiology of cardiac fibrosis involves various cellular effectors (neutrophils, macrophages, cardiomyocytes, fibroblasts), up-regulation of profibrotic mediators (cytokines, chemokines, and growth factors), and processes where epithelial and endothelial cells undergo mesenchymal transition. Activated fibroblasts and myofibroblasts are the central cellular effectors in cardiac fibrosis, serving as the main source of matrix proteins. The most effective anti-fibrotic strategy will have to incorporate the specific targeting of the diverse cells, pathways, and their cross-talk in the pathogenesis of cardiac fibroproliferation. Additionally, renalase, a novel protein secreted by the kidneys, is identified. Evidence demonstrates its cytoprotective properties, establishing it as a survival element in various organ injuries (heart, kidney, liver, intestines), and as a significant anti-fibrotic factor, owing to its, in vitro and in vivo demonstrated pleiotropy to alleviate inflammation, oxidative stress, apoptosis, necrosis, and fibrotic responses. Effective anti-fibrotic therapy may seek to exploit renalase’s compound effects such as: lessening of the inflammatory cell infiltrate (neutrophils and macrophages), and macrophage polarization (M1 to M2), a decrease in the proinflammatory cytokines/chemokines/reactive species/growth factor release (TNF-α, IL-6, MCP-1, MIP-2, ROS, TGF-β1), an increase in anti-apoptotic factors (Bcl2), and prevention of caspase activation, inflammasome silencing, sirtuins (1 and 3) activation, and mitochondrial protection, suppression of epithelial to mesenchymal transition, a decrease in the pro-fibrotic markers expression (’α-SMA, collagen I, and III, TIMP-1, and fibronectin), and interference with MAPKs signaling network, most likely as a coordinator of pro-fibrotic signals. This review provides the scientific rationale for renalase’s scrutiny regarding cardiac fibrosis, and there is great anticipation that these newly identified pathways are set to progress one step further. Although substantial progress has been made, indicating renalase’s therapeutic promise, more profound experimental work is required to resolve the accurate underlying mechanisms of renalase, concerning cardiac fibrosis, before any potential translation to clinical investigation.

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Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

Rahman

Rasid

Ali

et al. 2023

Journal of Nanotechnology

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This study synthesized silver nanoparticles (Ag-NPs) using silver nitrate (AgNO3) as the ion source and sodium tripolyphosphate (STPP) as reducing as well as capping agents. The synthesized Ag-NPs were confirmed initially using Ag-NPs specific λmax at 410 nm with UV-Vis spectrophotometry and homogenously distributed, 100–300 nm size, and round-shaped particles were realized through atomic force microscopy (AFM) and transmission electron microscopy (TEM) image analysis. The various reaction condition-based studies revealed 0.01 M AgNO3 yields maximum particle after 4 h reduction with 1% STPP. Bacillus spp. (n = 23/90) and Pseudomonas spp. (n = 26/90) were isolated from three different poultry farms for evaluating the antibacterial activity of Ag-NPs. Among the PCR confirmed isolates, 52% (12/23) Bacillus spp. were resistant to ten antibiotics and 65% (17/26) Pseudomonas spp. were resistant to eleven antibiotics. The representative resistant isolates were subjected to antibacterial evaluation of synthesized Ag-NPs following the well diffusion method, revealing the maximum sensitive zone of inhibition 19 ± 0.2 mm against Bacillus spp. and 17 ± 0.38 mm against Pseudomonas spp. The minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of Ag-NPs were 2.1 μg/ml and 8.4 μg/ml, respectively, for broad-spectrum application. Finally, the biocompatibility was determined by observing the viability of Ag-NP-treated BHK-21 cell through trypan blue-based exclusion assay revealing nonsignificant decreased of cell viability ≤2MIC doses. Thus, the synthesized Ag-NPs were proven as biocompatible and sensitive to both Gram-positive and Gram-negative bacteria of the poultry farm environmental samples.

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Rescue of human corneal epithelial cells after alkaline insult using renalase derived peptide, RP-220

Cited by 6 publications

References 33 publications

Renalase: A Multi-Functional Signaling Molecule with Roles in Gastrointestinal Disease

Renalase: A Multi-Functional Signaling Molecule with Roles in Gastrointestinal Disease

The Scientific Rationale for the Introduction of Renalase in the Concept of Cardiac Fibrosis

Facile Synthesis and Application of Ag-NPs for Controlling Antibiotic-ResistantPseudomonas spp. and Bacillus spp. in a Poultry Farm Environment

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